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《Genomics and Applied Biology》 2010-04
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Cloning, Expression Pattern and Bioinformatic Analysis of High-Affinity Potassium Transporter Gene SbHKT1 from Halophyte Salicornia bigelovii

Gao Yuanyuan Zhang Baolong Yang Yuwen Shen Xinlian Ni Wanchao Institute of Biotechnology,Jiangsu Academy of Agricultural Sciences,Nanjing,210014  
A putative high-affinity potassium transporter gene SbHKT1 was isolated from halophyte Salicornia bigelovii by RACE cloning.Sequence anlysis showed that the gene was 1 723 bp in whole length,has an open reading frame (ORF) of 1 647 bp encoding a 62.10 kD protein with 548 amino acids and theoretical pI of 9.33.Amino acid sequence from 1 to 35 is signal peptide sequence,and from 197 to 537 is TrKH characteristic sequence.The protein encoded by SbHKT1 gene contains 10 transmembrane domains.Then the tranmembrane area near N-end and the outer membrane area in middle of the peptide show obvious hydrophobicity some transmembrane areas near C-end and in middle of peptide show high hydrophobicity.Since these characters are similar to the features of cation transporter,and we predicted SbHKT1 was a transmembrane peptide.The Blast result showed the amino acid sequence had 77% homology with Suaeda salsa SsHKT1 and shared homology 63% with Mesembryanthemum crystallinum McHKT1,52% with Eucalyptus camaldulensis EcHKT1,46% with Triticum aestivum TaHKT1.The tissue-specific expression pattern of the SbHKT1 showed that under normal living conditions,its transcripts were detected weakly in roots and stems,and nearly no expression in leaves.However,under high salt and low potassium environment,the expression of SbHKT1 was increased obviously.The expression of SbHKT1 reached at peak in roots after being treated with high salt and potassium starvation for 8 h,100 μmol/L ABA for 4 h,and 20% PEG6000 for 2 h.All of these results demonstrate SbHKT1 takes part in plant physiologic regulations under some abiotic stress such high salt /low potassium environment,osmotic stress and drought stress.Recently,most of the genes of HKT-like protein were cloned from non-halophyte.There is rare research about the HKT gene of halophyte.So the full length SbHKT1 gene cloning from Salicornia bigelovii helps people focus on the potassium transporting and Na+/K+ balancing function of this gene under high salt with potassium starvation environment.It is significant to uncover the salt tolerance mechanism of halophyte,to apply this mechanism to non-halophyte and to breed salt tolerant varieties ultimately.
【Fund】: 国家转基因专项(2008ZX08005-004和2008ZX08005-001);; 江苏省“六大人才高峰”项目共同资助
【CateGory Index】: Q943.2
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【Citations】
Chinese Journal Full-text Database 4 Hits
1 ZHANG Da-dong~(1,2),MA Hong-xiang~(1),JI Xiao-jia~(2), ZHOU Chun-lin~3,WANG Mao-wen~3,TANGRi-sheng~(1), YANG Yong-hua~(2)(1.Institute of Agrobiological Genetics and Physiology,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China;2.State Key Laboratory of Pharmaceutical Biotechnology,College of Life Science,Nanjing University,Nanjing 210093,China;3.Institute of Agricultural Sciences in Coastal District of Jiangsu,Yanchen 224002,China);Construction of cDNA Library of Salicornia bigelovii Torr.during Early Stage of Salt Stress Based on Suppression Subtractive Hybridization[J];Jiangsu Journal of Agricultural Sciences;2006-02
2 SHAO Qun~1,HAN Ning~(1,2),DING Tong-Lou~1,WANG BaoShan~1~~*(1.College of Life Science,Shandong Normal University,Ji'nan 250014,China;2.College of Food and Biologic Engineering,Shandong Institute of Light Industry,Ji'nan 250100,China);Polyclonal Antibody Preparation and Expression Analysis of High-affinity K~+ Transporter SsHKT1[J];Journal of Wuhan Botanical Research;2006-04
3 ZHAO Kefu ?&? FAN Hai ( Shandong Normal University, Shandong Engineering and Technology Center for Halophytes , Jinan 250014);COMPARATIVE STUDY ON OSMOTICA AND THEIR CONTRIBUTIONS TO OSMOTIC ADJUSTMENT IN EU HALOPHYTES AND RECRETOHALOPHYTES[J];CHINESE JOURNAL OF APPLIED AND ENVIRONMENTAL BIOLOGY;2000-02
4 ZHOU Feng, LI Ping-Hua, WANG Bao-Shan (College of Life Science, Shandong Normal University, Jinan 250014);The Relationship Between the K~+ Homeostasis and Plant Salt Tolerance[J];Plant Physiology Communications;2003-01
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 LIU Guo-hua (Department of Life Science,Chongqing University of Arts and Sciences, Chongqing 402168);Research Progress in Mechanism of Plant Resistance to Salt Stress[J];Journal of Anhui Agricultural Sciences;2006-23
2 DONG Bi-hui et al(Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection,Yancheng,Jiangsu 224002);Primary Sutdy on the Anatomic Structure and the Photosynthesis Characters of Limonium sinense[J];Journal of Anhui Agricultural Sciences;2007-09
3 ZHAO Chang et al (College of Life Science,Shandong Normal University, Jinan,Shandong 250014);Function of High Affinity K~+ Transporter in the Tolerance of Plant to Salt[J];Journal of Anhui Agricultural Sciences;2007-12
4 ZHOU Feng et al(Department of Life Science,Nanjing Xiaozhuang College,Nanjing,Jiangsu 211171);Effects of Na_2CO_3 and NaCl Treatments on the Growth and Antioxidant Enzyme Activity of Salicornia europaea[J];Journal of Anhui Agricultural Sciences;2007-36
5 SUN Hui et al(Department of Plant Protection,Zhongkai University of Agriculture and Engineering,Guangdong,Guangzhou 510225);Application of Subtractive cDNA Library in Study of Plant Resistance[J];Journal of Anhui Agricultural Sciences;2010-05
6 DU Li-xia,DONG Kuan-hu,XIA Fang-shan,DONG Qiu-li(College of Animal Science and Veterinary Medicine,Shanxi Agricultural University,Taigu,Shanxi Province 030801,China);Effect of Salt Stress on Germination and Physiological Characteristics of Russian Wildryegrass Seed[J];Acta Agrestia Sinica;2009-06
7 CHEN Tuo-xiong,ZHANG Jin-lin,LU Ni,WANG Suo-min (School of Pastoral Agriculture Science and Technology and the Key Laboratory of GrasslandAgro-ecosystem of China Agricultural Ministry,Lanzhou University,Lanzhou 730000,China);The characteristics of free proline distribution in various types of salt-resistant plants[J];Acta Prataculturae Sinica;2006-01
8 ZHENG Qing-song1,2,HUA Chun3,DONG Xian1,LI Xiu-hua1,CHEN gang2,4(1.College of Natural Resources and Environmental Science,Nanjing Agricultural University,Nanjing 210095,China;2.Key Laboratory of Crop Cultural Physiology of Jiangsu Province,Yangzhou University,Yangzhou 225009,China;3.Department of Life Science,Xiaozhuang University,Nanjing 210017,China;4.College of Bioscience and Biotechnology,Yangzhou University,Yangzhou 225009,China);Study of characteristic response to salt-ion stresses of Salicornia europaea seedlings[J];Acta Prataculturae Sinica;2008-06
9 Wang Lei(College of Life Sciences,Northeast Agricultural University,Harbin 150030,P.R.China);Xu Dechang(Harbin Institute of Technology);Zhu Yanming,Bai Xi(Northeast Agricultural University);Ion Homeostasis Regulatory Genes and Their Application to Genetic Engineering of Salinity Tolerance in Plants[J];Journal of Northeast Forestry University;2008-06
10 WANG Li-yan,ZHANG Hong (Department of Biology,Dezhou University,Dezhou Shandong 253015 ,China);Effect of NaCl stress on the content of inorganic ions in wild soybean[J];Journal of Dezhou University;2004-02
China Proceedings of conference Full-text Database 2 Hits
1 Du Lixia Dong Kuanhu (College of Animal Science and Technology,Shanxi Agricultural University,Shanxi province,Taigu,China 030801);Advances in Research on the Breeding of Salt-tolerant Plants[A];[C];2007
2 WU Xiang,NI Jianwei,ZHANG Huaxin,LIU Tao,ZHANG Li (Research Institute of Forestry,Chinese Academy of Forestry,Beijing 100091,China);Effects of Salt Stress on Growth and Physiological Index of 3 Nitraria Species[A];[C];2011
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 ZHU Du, XU Qu (Institute of Chemistry and Biology Sciences, Jiangxi Normal University, Nanchang 330027,China);Analysis on Fatty Acids in Celosia Argentea L. Seed Oil by Gas Chromatography[J];Journal of Jiangxi Normal University (Natural Sciences Edition);2002-02
2 HU Juan1,QIU Hui-zhen1,HE Xiu-cheng1,ZHANG Wen-ming1, LI Ya-juan2,ZHANG Chun-hong1 (1.College of Resources and Environmental Sciences,Gansu Agricultural University,Lanzhou 730070, China;2.College of Prataculture,Gansu Agricultural University,Lanzhou 730070,China);Effects of potassium fertiliser on potassium content in flue-cured tobacco leaves and the economic benefits in Gansu province[J];Acta Prataculturae Sinica;2010-05
3 LU Li-ming,YANG Tie-zhao~* (Agronomy College of Henan Agricultural University,Zhengzhou 450002,China);Potassium Transporters and Their Molecular Regulation in Higher Plants[J];Acta Botanica Boreali-Occidentalia Sinica;2006-11
4 SUN Bo1,PAN Xianzhang1,WANG Dejian1,HAN Xiaozeng2,ZHANG Yuming3,HAO Mingde4,CHEN Xin5(1.Institute of Soil Science,CAS,Nanjing 210008,China;2.Northeast Institute of Geography and Agro-Ecology,Chinese Academy of Sciences,Harbin 150040,China;3.Institute of Genetics and Developmental Biology,Institute of Geographical Sciences and Natural Resources Research,CAS,Shijiazhuang 050021,China;4.Institute of Water and Soil Conservation,Chinese Academy of Sciences,and Ministry of Water Resources,Yangling Demonstration Area 712100,China;5.Institute of Applied Ecology,CAS,Shenyang 110016,China);Effect of Nutrient Balance on Spatial and Temporal Change of Soil Fertility in Different Agriculture Area in China[J];Advances in Earth Science;2008-11
5 DENG Xiao-bo,HU Shang-lian,CAO Ying,LU Xue-qin,REN Peng,DUAN Ning (College of Life Science and Engineering,Southwest University of Science and Technology,Mianyang,Sichuan 621010,China);Bioinformatics analysis of cellulose synthase(CesA) of Bambusa oldhamii and Phyllostachy edulis[J];Journal of Fujian College of Forestry;2011-01
6 YANG Tie-zhao,XIA Wei, FAN Jin-hua (Agronomy Department, Henan Agriculture University, Zhengzhou 450002, China);Study on characteristic of potassium accumulation of tobacco in potassium levels[J];Henan Science;2005-03
7 PENG Zhi hong,PENG Ke qin,HU Jia jin,XIAO Lang tao (Key Laboratory of Phytohormones,HNAU,Changsha 410128,PRC);Screening of K-Deficit Tolerant Offspring from Different Rice Varieties Introduced DNA of K-Sufficiency Plant[J];Journal of Hunan Agricultural University;2002-06
8 ZHANG Da-dong~(1,2),MA Hong-xiang~(1),JI Xiao-jia~(2), ZHOU Chun-lin~3,WANG Mao-wen~3,TANGRi-sheng~(1), YANG Yong-hua~(2)(1.Institute of Agrobiological Genetics and Physiology,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China;2.State Key Laboratory of Pharmaceutical Biotechnology,College of Life Science,Nanjing University,Nanjing 210093,China;3.Institute of Agricultural Sciences in Coastal District of Jiangsu,Yanchen 224002,China);Construction of cDNA Library of Salicornia bigelovii Torr.during Early Stage of Salt Stress Based on Suppression Subtractive Hybridization[J];Jiangsu Journal of Agricultural Sciences;2006-02
9 Wang Yongrui;Li Weijun;Yu Kuanjing(1 College of Life Sciences.Sun Yatsen University,Guangzhou 510275;2 State Farm of Haifeng County,Guangdong Province 516400);SCREENING RICE VARIETIES(Oryza sativa L.)OF GEN0IYPE TOLAkANT TO K DEFICIENCY WITH NUTRITON SOLUTION CULTURE[J];ACTA AGRICULTURAE UNIVERSITIS JIANGXIENSIS;1996-02
10 WANG Xiaofeng(China National Pertrolem and Chemical Planning Institute, Beijing 100013, China);The potash resources at home and abroad and current status of their exploitation and utilization[J];Phosphate & Compound Fertilizer;2005-01
【Secondary Citations】
Chinese Journal Full-text Database 8 Hits
1 Liu Weihong 1 Liu Feihu 2 D. Schachtman 1(1Plant Industry, CSIRO at Adelaide, SA, Australia;2College of Agronomy, JAU, Nanchang330045);Potash Ion Transport Mechanism and its Molecular Basis in Plant Root Cells[J];ACTA AGRICULTURAE UNIVERSITIS JIANGXIENSIS;1999-04
2 LIU Zhi,YANG Yong-hua College of Life Sciences,Nanjing University,Nanjing210093,China;Extraction of total RNA from Onosma paniculatum,a Chinese medical plant,by CTAB method[J];Letters in Biotechnology;2004-04
3 XIE Shao-Ping Shanghai Institute of Plant Physiology, Academia Sinica, Shanghai 200032;Regulation of Potassium Absorption in Higher Plants[J];Plant Physiology Communications;1989-04
4 ZHOU Rong-Ren;YANG Shi-Rong;YU Shu-Wen Shanghai Institute of Plant Physiology, Academia Sinica, Shanghai 200032;Research on Mechanism of Salt Tolerance and Selection cf Salt Tolerant Mutant in Plants by Tissue Culture[J];Plant Physiology Communications;1989-05
5 XIONG Ying-Jie1,ZHAO Li-Qun2,ZHONG Tao-Tao1,AN Chang-Ting1,LIANG Rong1,SUN Bao-Teng1,1College of Life Sciences and Food Engineering,Nanchang University,Nanchang 330031,China;2School of Life Sciences,Hebei Normal University,Shijiazhuang 050016,China;Effects of Exogenous Nitric Oxide on the Growth and Ion Homeostasis of Maize (Zea mays L.) Seedlings under Magnesium Deficient Stress[J];Plant Physiology Communications;2010-07
6 WANG Bao_Shan 2ZOU Qi 1( Institute of Plant Stress, Shandong Teachers' University, Jinan 250014) 2 College of Life Science, Shandong Agricultural University, Tai'an 271018);Advances in the study on Plasma Membrane-bound Translocating Proteins and Their Relations with Salt Tolerance in Plants[J];CHINESE BULLETIN OF BOTANY;2000-01
7 QIU Quan_Sheng Department of Biology, Beijing Normal University, Beijing 100875);Research Progress on the Plasma Membrane Potassium Ion Transporters[J];CHINESE BULLETIN OF BOTANY;2000-01
8 LIU Jian_Xiang 1YANG Xiao_E 1WU Liang_Huan 2John Bennett 1(College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310029) 2(Division of Plant Breeding, Genetics and Biochemistry, International R;The Relationship between Membrane Transport Systems and Potassium Use Efficiency in Plants[J];Chinese Bulletin of Botany;2001-05
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