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《Genomics and Applied Biology》 2010-04
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Cloning, Expression Pattern and Bioinformatic Analysis of High-Affinity Potassium Transporter Gene SbHKT1 from Halophyte Salicornia bigelovii

Gao Yuanyuan Zhang Baolong Yang Yuwen Shen Xinlian Ni Wanchao Institute of Biotechnology,Jiangsu Academy of Agricultural Sciences,Nanjing,210014  
A putative high-affinity potassium transporter gene SbHKT1 was isolated from halophyte Salicornia bigelovii by RACE cloning.Sequence anlysis showed that the gene was 1 723 bp in whole length,has an open reading frame (ORF) of 1 647 bp encoding a 62.10 kD protein with 548 amino acids and theoretical pI of 9.33.Amino acid sequence from 1 to 35 is signal peptide sequence,and from 197 to 537 is TrKH characteristic sequence.The protein encoded by SbHKT1 gene contains 10 transmembrane domains.Then the tranmembrane area near N-end and the outer membrane area in middle of the peptide show obvious hydrophobicity some transmembrane areas near C-end and in middle of peptide show high hydrophobicity.Since these characters are similar to the features of cation transporter,and we predicted SbHKT1 was a transmembrane peptide.The Blast result showed the amino acid sequence had 77% homology with Suaeda salsa SsHKT1 and shared homology 63% with Mesembryanthemum crystallinum McHKT1,52% with Eucalyptus camaldulensis EcHKT1,46% with Triticum aestivum TaHKT1.The tissue-specific expression pattern of the SbHKT1 showed that under normal living conditions,its transcripts were detected weakly in roots and stems,and nearly no expression in leaves.However,under high salt and low potassium environment,the expression of SbHKT1 was increased obviously.The expression of SbHKT1 reached at peak in roots after being treated with high salt and potassium starvation for 8 h,100 μmol/L ABA for 4 h,and 20% PEG6000 for 2 h.All of these results demonstrate SbHKT1 takes part in plant physiologic regulations under some abiotic stress such high salt /low potassium environment,osmotic stress and drought stress.Recently,most of the genes of HKT-like protein were cloned from non-halophyte.There is rare research about the HKT gene of halophyte.So the full length SbHKT1 gene cloning from Salicornia bigelovii helps people focus on the potassium transporting and Na+/K+ balancing function of this gene under high salt with potassium starvation environment.It is significant to uncover the salt tolerance mechanism of halophyte,to apply this mechanism to non-halophyte and to breed salt tolerant varieties ultimately.
【Fund】: 国家转基因专项(2008ZX08005-004和2008ZX08005-001);; 江苏省“六大人才高峰”项目共同资助
【CateGory Index】: Q943.2
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