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《Genomics and Applied Biology》 2011-05
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Full-length cDNA Cloning,Sequence Homology Analysis and Tissue Expression of a Catalase Gene from Grass Carp(Ctenopharyngodon idellus)

Zheng Qingmei * Han Chunyan Wen Rushu Zhong Yanmei Yao Qiongfeng Hou Yuwen College of Life Science,Jiaying University,Meizhou,514015  
Catalase is a key enzyme in the antioxidant systems of living organisms that plays an important role in the against oxidative stress by eliminating hydrogen peroxide.The full-length catalase cDNA was cloned from hepatopancreas of grass carp(Ctenopharyngodon idellus).The gene CAT(GenBank Accession No.FJ560431) was 2 263 base-pairs(bp),including a complete protein coding region(ORF) of 1 575 bp,a 5' untranslated region(UTR) of 118 bp and 3' UTR of 570 bp.The ORF encoded 525 amino acid(aa) residues and the molecular mass of the predicted protein was 59.59 kD with an estimated pI of 7.02.The 3' UTR of the cDNA contained a dinucleotide repeat near the termination codon consisting of a near perfect and long CA array,which had the same characteristic of catalase from zebrafish,silver carp and rodents.Sequence comparison showed that the nucleotide sequence and the deduced amino acid sequence of grass carp CAT shared 93.4%~43.0% and 98.1%~63.3%,respectively,identity with that of other selected species.Several highly conserved motifs including the proximal heme-ligand signature sequence "RLFSYPDTH",the proximal active site signature "FDRERIPERVVHAKGA",and the three catalytic amino acid residues of His74,Asn147 and Tyr357 were identified in the deduced amino acid sequence of CAT from grass carp.Besides the CAT also had highly conserved heme binding pocket and NADPH binding site.Accordingly above-mentioned sequence signature,the grass carp CAT was presumed to be monofunctional CAT or typical CAT in the CAT family.CAT transcripts were detected in the tissue of grass carp.And variance mRNA abundance was detected in different tissue.Real-time quantitative PCR(Q-PCR) method was used to analyze the CAT mRNA expression characterization in tissues of grass carp.The results showed expression of CAT mRNA was found in all detected 11 species of tissues and which was high in the tissues of liver and low in the tissues of red muscle,white muscle and fat.Therefore,this study would be contributed to the discuss genetic structure and function of the fish CAT,and lay the foundation of CAT molecular mechanism of antioxidation.
【Fund】: 广东高校优秀青年创新人才培育项目(LYM08093);; 广东省自然科学基金项目(S2011010003451)共同资助
【CateGory Index】: S917.4;Q78
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【Citations】
Chinese Journal Full-text Database 6 Hits
1 ZHANG Ke-Feng ZHANG Zi-Ping ** CHEN Yun LIN Peng WANG Yi-Lei* (The Key Laboratory of Science and Technology for Aquaculture and Food Safety,Fisheries College, Jimei University,Xiamen 361021,China );Antioxidant Defense System in Animals[J];Chinese Journal of Zoology;2007-02
2 Zheng Qingmei 1,2 Wen Xiaobo 1 Han Chunyan 2 Li Haobo 1 1 College ofAnimal Science, South China Agricultural University, Guangzhou, 510642; 2 College ofLife Science, Jiaying University, Meizhou, 514015;Full-length cDNA Cloning and Analysis of Glutathione Peroxidase from Grass Carp (Ctenopharyngodon idellus)[J];Genomics and Applied Biology;2010-05
3 LIU Ling-zhi1,2,ZHONG Guang-rong1,XIONG Lian1,2,CHANG Yan-hong2,XIAO Bao-qing2,LUO Hui1(1.Department of Biological Science and Technology,University of Science and Technology Beijing,Beijing 100083,China;2.Department of Environmental Engineering,University of Science and Technology Beijing,Beijing 100083,China);Research and Application Progress of Catalase[J];Chemistry & Bioengineering;2009-03
4 Zhao Yuanfeng Lu Jingcai Song Xiaoyang Xu Qingling Liu Hengming You Xuece Hou Meiyan Sun Jing Pan Dejian(Deptartement of Aquaculture, Dalian Fishery University, Dalian 116023 , China);Effect of Cadmium on Activities of Superoxide Dismutase and Catalase in Aristchthys nobilis[J];Journal of Agricultural Biotechnology;2002-03
5 XU Dengxian,SHEN Haitao,WANG Aiying,ZHU Jianbo(Key Laboratory of Agricultural Biotechnology,College of Life Science,Shihezi University,Shihezi 832003,China);Cloning and Construction of Plant Express Vector of Escherichia Coli katE Gene and Transgenic in Tobacco[J];Journal of Shihezi University(Natural Science);2009-02
6 WANG Gailing 1,2,LUO Yanping 1,2,SUN Baojian 1,XU Zhen 1,XU Qiaoqing 1,NIE Pin 1(1.State Key Laboratory of Freshwater Ecology and Biotechnology,Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan 430072,China;2.Graduate School of Chinese Academy of Sciences,Beijing 100039,China);Cloning and expression of immunoglobulin D heavy chain in mandarin fish,Siniperca chuatsi[J];Journal of Fishery Sciences of China;2010-01
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 Li yeyu et al.(Department of life science,Nanjing Xiaozhuang college,Nanjing 211171,China);Bioinformatic Analysis of catalase Gene from Hypericum perforatum[J];Anhui Agricultural Science Bulletin;2009-13
2 TIAN Jing-Yun,XIE Hai-Xia,YAO Wei-Jian,NIE Pin  State Key Laboratory of Freshwater Ecology and Biotechnology,Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan 430072,China;Ontogenetic development of the pronephros and the distribution of B-lymphocytes in adult pronephros of the mandarin fish Siniperca chuatsi[J];Acta Zoologica Sinica;2005-03
3 QIAO Shu-Jing FAN Xue-Ming FANG Fang CHEN Feng-Hu CHEN Hui (Department of Biology,College of Life and Environment Sciences, Harbin Normal University,Harbin 150080,China);The Poisoning of Metriphonate to Limnodilus hoffineisteri[J];Chinese Journal of Zoology;2006-06
4 JIAO Chuan-zhen(Department of Biology,Dezhou University,Dezhou 253023,China);The Effect of Cerium on SOD and POD Activities in Hepatopancreas of Oriental Weatherfish(Misgurnus anguillicaudatus)Stressed by Cadmium[J];Fisheries Science;2009-12
5 WANG Fan 1,LIU Hai-fang2,JIN Dong-mei1,WANG Peng1,SUN Kun-gang1,ZHANG Shui-tu1(1.Life Science Department,Luoyang Normal University,Luoyang 471022,China;2.School of Energy & Environmental Engineering,Zhongyuan University of Technology,Zhengzhou 450007,China);Effect of Cadmium on Catalase Activities in Common Carp,Cyprinus carpio[J];Fisheries Science;2010-09
6 ZHENG Qing-mei1,GUO Jiang-shan2,WEN Ru-shu1,HAN Chun-yan1(1.College of Life Science,Jiaying University,Meizhou 514015,China;2.Meizhou Fisheries Research Institute,Meizhou 514071,China);Effects of High Concentration Pb~(2+) on Antioxidant Capability in Juvenile Grass Carp(Ctenopharyngodon idellus)[J];Fisheries Science;2010-11
7 ZHANG Long-zhen1,2,JIANG Qi1,2,ZHUANG Ping1,2,3,HUANG Xiao-rong1,2,YAN Wen-gang1,2(1.East China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Key and Open Laboratory of Marine and Estuarine Fisheries Resources and Ecology,Ministry of Agriculture,Shanghai 200090,China;2.College of Marine Sciences,Shanghai Ocean University,Shanghai 201306,China;3.Aquaculture Division,E-Institute of Shanghai Universites,Shanghai 201306,China);Effects of cryopreservation on antioxidant activities of semen enzymes in Russian sturgeon Acipenser gueldenstaedti[J];Journal of Dalian Fisheries University;2009-06
8 LIANG Wen-yu1,2,JIAO Guang-fei1,YOU Xiang-rong1,ZHOU You-wen2,CHEN Wei1(1 College of Life Sciences,Fujian Agriculture and Forestry University,Fuzhou 350002,China;2 School of Life Sciences,Ningxia University,Yinchuan 750021,China);Differential Expression and Gene Cloning of Mn-CAT from Nostoc flagelliforme Subjected to Desiccation and Rehydration[J];Acta Botanica Boreali-Occidentalia Sinica;2011-02
9 YANG Fan,SUN Han-chang(College of Life Science and Technology,University of Arts and Sciences,Chongqing 402168);Effects of linear alkylbenzene sulfonate(LAS) on the activities of superoxide dismutase and glutathione peroxidase in Spinibarbus sinensis[J];Freshwater Fisheries;2009-05
10 Zheng Qingmei 1,2 Wen Xiaobo 1 Han Chunyan 2 Li Haobo 1 1 College ofAnimal Science, South China Agricultural University, Guangzhou, 510642; 2 College ofLife Science, Jiaying University, Meizhou, 514015;Full-length cDNA Cloning and Analysis of Glutathione Peroxidase from Grass Carp (Ctenopharyngodon idellus)[J];Genomics and Applied Biology;2010-05
China Proceedings of conference Full-text Database 1 Hits
1 CHEN Wei~1,LI Hua~1,ZENG Yong-qing~1,YANG Qi-de~2,CHAI Qing-hua~2, XIA Guang-shun~3,LI Xiang-de~3 (1College of Animal Science and Technology,Shandong Agricultural University, Taian 271018,P.R.China; 2Shandong Shouguang Vegetable Exchange Company,Shouguang 262700,P.R.China; 3Shandong Shouguang Animal Husbandry Bureau,Shouguang 262700,P.R.China);Effects of supplemental fresh garlic in diet on muscle antioxidant ability and meat quality properties for pigs[A];[C];2009
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Nan zhirun fan yuexian(Department of art and science,shanxi agriculture of university,taigu 030801);Advance of Researches on Catalase in Plants[J];Anhui Agricultural Science Bulletin;2008-05
2 ZHANG Ke-Feng ZHANG Zi-Ping ** CHEN Yun LIN Peng WANG Yi-Lei* (The Key Laboratory of Science and Technology for Aquaculture and Food Safety,Fisheries College, Jimei University,Xiamen 361021,China );Antioxidant Defense System in Animals[J];Chinese Journal of Zoology;2007-02
3 HE Shan LIANG Xu-Fang LIAO Wan-Qin YAO Wei LI Guang-Zhao ZHU Wei-Feng(College of Life Science and Technology,Jinan University,Guangzhou 510632,China);Molecular Cloning and Tissue Expression of Glutathione Peroxidase cDNA in Hypophthalmichthys molitrix,Aristichthys nobilis and Ctenopharyngodon idella[J];Chinese Journal of Zoology;2007-03
4 CHEN Jin-feng1,WANG Gong-nan1,CHENG Su-man2(1 College of Chemical Engineering Kaifeng University,Kaifeng,Henan 475004,China;2 The Fifth People's Hospital of Kaifeng City,Kaifeng,Henan 475003,China);Progress about Catalase Function in Plant Stress Reactions[J];Acta Botanica Boreali-Occidentalia Sinica;2008-01
5 WANG Zhi-hua,SHEN Liang(School of Material and Chemical Engineering,Hangzhou Teachers College,Hangzhou 310036,China);Manganese Catalase and Their Analogue Study Progress[J];Journal of Hangzhou Teachers College(Natural Sciences Edition);2006-06
6 Zhang Wen, Luo Qinhui(7);Research Progress in the Manganese Catalase and Its Model Complexes[J];CHEMISTRY;2000-10
7 ;Extraction of Cotton Total DNA Using Development SDS Methods for mini-sample[J];Acta Gossypii Sinica;2005-05
8 CAO Yanlin1,2,KE Hao1,LIU Zhenxing1,ZHANG Jianfei1,LIN Min1(1.Guangdong Public Lab.of Veterinary Public Health,Institute of Veterinary Medicine,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,China;2. College of Life Sciences,South China Agricultural University,Guangzhou 510642,China);Molecular cloning and characterization analysis of glutathione peroxidase 1 from Nile tilapia (Oreochromis niloticus)[J];South China Fisheries Science;2010-03
9 TANG Xue xi 1, ZHANG Pei yu 2 (1.Life Science College,Ocean University of Qingdao,Qingdao 2660030,China; 2.Biology Department,Qufu Normal University,Qufu 273165,China);Effects of anthracene on activity of superoxide dismutase in Sebastodes fuscescens[J];JOURNAL OF FISHERIES OF CHINA;2000-03
10 FANG Fang 1,2 LI Yin 1,2 DU Guo-Cheng 2 ZHANG Juan 1,2 CHEN Jian 1,2* 1(Key Laboratory of Industrial Biotechnology, Ministry of Education, Southern Yangtze University, Wuxi, 214036,China) 2(Environmental Biotechnology Laboratory, School of biotechnology, Southern Yangtze University,Wuxi, 214036,China);Thermo-alkali-stable Catalase from Thermoascus aurantiacus and Its Potential Use in Textile Bleaching Process[J];Chinese Journal of Biotechnology;2004-03
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