Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Genomics and Applied Biology》 2012-05
Add to Favorite Get Latest Update

Study on Isolation and Identification of a Bacillus Strain Producing Normal Temperature Glucomannase and Fermentation Conditions for Enzyme Production

Xu Jian 1,2,3 Sun Xuezhe 1,2,3 Chen Qiang 1,2,3 Pang Zongwen 1,2 Song Xianchong 1,2,3 Zhang Jie 4 Luo Di 4 Li Youzhi 1,2,3 1 State Key Laboratory of Conservation and Utilization of Subtropical Agro-bioresources, Nanning, 530005; 2 College of Life Science and Technology of Guangxi University, Nanning, 530005; 3 State key Laboratory of Microbial and Plant Genetic Engineering of Guangxi University, Nanning, 530005; 4 Guilin Welpont Biotechnology Co. Ltd, Guilin, 541004  
A bacterial strain degrading raw konjac flour, numbered LS-6, was isolated from waste konjac residues on the isolation media plate with raw konjac flour as a single carbon source. By analyses of 16S rDNA sequence, and physiological and biochemical characteristics, LS-6 was identified as Bacillus subtilis. As a result of analyses of extra and intra cellular glucomannanase, the glucomannase produced by LS-6 belongs to the extracellular enzyme. The optimum components for production of the glucomannase by this strain were 1% raw konjac flour as carbon source, 0.5% Yeast extract as nitrogen source, 0.5% NaCl, initial pH 6.0 for the medium, and that the optimum fermentation conditions was for 48 h at 25℃ at 200 r/min. Analysis from high performance liquid chromatography indicated that major enzymatic products of raw konjac flour by crude glucomannanase produced by fermentation with LS-6 included glucose and mannose. The isolation of LS-6 lays a foundation for further cloning of room-temperature glucomannanase as well as construction of engineering bacteria producing the enzyme.
【Fund】: 国家科技部科技人员服务企业行动项目“酶工程法生产魔芋甘露低聚糖产业化科技服务示范(编号:2009GJE10020)”资助
【CateGory Index】: TQ925
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 9 Hits
1 Meng Hai-lin, Zhang Yun-kai, Ling Min, Chen Gui-guang, Liang Zhi-qun (College of Life Science and Technology, Guangxi University,Nanning 530004, China);Breeding of β-mannanase Producing Strains[J];Modern Food Science and Technology;2006-02
2 CHEN Li 1, YANG Yan yan 2, YAN Da zhong 2 (1. Department of biochemistry, Chongqing Medical Staff College, Chongqing 400050, China; 2. School of Life Sciences, Hubei University, Wuhan 430062, China );The effects of konjac oligosaccharides on decreasing blood lipid[J];Chinese Journal of Biochemical Pharmaceutics;2002-04
3 Li Jianfang1 Wu Minchen2 Xia Wenshui1 1(School of Food Science and Technology, Southern Yangtze University, Wuxi, 214036, China) 2(Department of medicine, Southern Yangtze University, Wuxi, 214036, China);Breeding of Acidic β-Mannanase Overproducing Strain and Studies on Its Enzyme Production Conditions[J];Food and Fermentation Industries;2005-09
4 Cao Jinsong Wang Xiaoqin Peng Zhiying (Institute of Food & Biology Engineering, South China University of Technology, Guangzhou,510641);Problems and Strategies of Oligosaccharide Synthesis Using Microbial Enzymes[J];FOOD AND FERMENTATION INDUSTRIES;1999-04
5 Wu Yo ngjun et al.;Konjac -Glucomanna Extracted to Imp rove Growth of Bifidobacteriam[J];Food Science;2002-06
6 CHEN Xiu min, L Xiu ju, FU De xian , OUYANG Fan (National Laboratory of Biochemical Engineering of China, Institute of Process Engineering, The Chinese Academy of Sciences, Beijing 100080,China);The Preparation and Chemical Structure of Amorphophallus albus Konjac Oligosaccharides and Their Effects on NO Free Radical Release from the STZ-induced Diabetic Islets[J];Chinese Journal of Biochemistry and Molecular Biology;2002-06
7 Ma Yanhe Tian Xinyu Zhou Peijin Wang Dazhen (Institute of Microbiology,Academia Sinica,Beijing);PRODUCTION AND SOME PROPERTIES OF ALKALINE β-MANNANASE[J];Acta Microbiologica Sinica;1991-06
8 Cui Fumian\ Shi Jiaji\ Lu Zhuozhuang (Institute of Microbiology,Chinese Academy of Sciences, Beijing\ 100080);PRODUCTION OF NEUTRAL BATA MANNANASE BY BACILLUS SUBTILIS AND ITS PROPERTIES[J];ACTA MICROBIOLOGICA SINICA;1999-01
9 CHENG Li-feng,LIU Zheng-chu,ZHANG Yun-xiong,DUAN Sheng-wen, FENG Xiang-yuan,ZHENG Ke(Institute of Bast Fiber Crops,Chinese Academy of Agricultural Science,Changsha 410205,China);Study on Neutral β-Mannannase Secreted by CXJZ11-01 Strain[J];Plant Fiber Sciences in China;2007-05
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 NIE Guang-Jun,YUE Wen-Jin,LIN Xing-Xing,LU Xiao-Lan(Dept of Bioch.Engn.,Anhui University of Technology and Science,Wuhu 241000,China);Acetone precipitation of β-mannase[J];Journal of Anhui University of Technology and Science(Natural Science);2007-04
2 ZHU Jie et al(College of Medicine,Southern Yangtze University,Wuxi,Jiangsu 214064);Effect of the Xylooligosaccharides on Blood Sugar and Serum Lipid of Mice[J];Journal of Anhui Agricultural Sciences;2007-21
3 ZHANG Hui(College of life Science,Liaocheng University,Liaocheng,Shandong 252059);Conditions Optimization for the Production of Acidic β-Mannanase from One Strain of Penicillium spp.in Solid Fermentation[J];Journal of Anhui Agricultural Sciences;2008-05
4 SUN Zhan-bin et al(School of Life Science,Liaocheng University,Liaocheng,Shandong 252059);Studies on the Liquid Fermentation Condition of Production of Acidic β-mannanase from Penicillium sp.QM-1[J];Journal of Anhui Agricultural Sciences;2009-02
5 WANG Xi-sen et al (Institute of Bast Fiber Crops,Chinese Academy of Agriculture Sciences,Changsha,Hunan 410205);Study on the Construction of Special Compound Enzyme Engineering Strain for Bio-extracting Herbaceous Fiber[J];Journal of Anhui Agricultural Sciences;2009-09
6 ZHOU Hai-yan1, WU Yong-yao*, ZHOU Da-zhai2 (1. Lab of Biochemistry and Fermentation Engineering, Hanan Agricultural University, Changsha 410108, China; 2. Institute for Biological Technology, Hubei Institute for Nationalities, Enshi 445000, China);Preliminary Studies on Conditions of Experimental Production of Konjac Glucomannanase[J];Amino Acids & Biotic Resources;2005-02
7 LIU Nan1,YANG Fang1,2(1.College of Agriculture and Life Sciences,Ankang University,Ankang 725000,Shaanxi,China;2.Shaanxi Selenium-rich Food Engineering Lab,Ankang University,Ankang 725000,Shaanxi,China);The Research Progress and Application Status of Konjac Glucomannan[J];Journal of Ankang University;2011-04
8 ZHU Jie~1,LI Jian-fang~2,WU Min-chen~1(1.Department of Medicine,Southern Yangtze University,Wuxi 214064,China;2.School of Food Science,Southern Yangtze University,Wuxi 214064,China);Studies on purification and some properties of the acidic β-mannanase from Aspergillus niger[J];Journal of Agricultural University of Hebei;2007-01
9 QI Li 1, LI Guangji 1, ZONG Minhua 2 ( 1 Department of Polymer Science and Engineering; 2 Biotechnology Department, South China University of Technology, Guangzhou 510640);ENZYME-CATALYZED CONTROLLABLE DEGRADATION OF KONJAC GLUCOMANNAN[J];Acta Polymerica Sinica;2003-05
10 ZANG Cheng YUAN Hong-Zhi(Tongliao Institute of Product Quality & Measure Inspection,Tongliao,Innermongolia 028000,P.R.China);Wilted Hay Bacilli Producing α-Amylase in Amylum Culture Medium[J];Chinese Journal of Spectroscopy Laboratory;2008-05
China Proceedings of conference Full-text Database 1 Hits
1 WU Linjuan,PANG Jie (Food Science College,Fujian Agriculture and Forestry University,Fuzhou,350002,China);Research Advance of Anti-tumor Activity of Oligosac Charides[A];[C];2010
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Wen et al(College of Food Science and Technology,Shanghai Ocean University,Shanghai 201306);Identification of Mannanase-producing Bacterial Strain F1-5[J];Journal of Anhui Agricultural Sciences;2009-04
2 Xu Guiyun; Chang Liwen(Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080);Structural Elucidation of Di-and Trisaccharides from Enzymatic Hydrolysates of Konjak Glucomannan by Methylation Analysis and Fast Atom Bombardment Mass Spectrometry[J];Chinese Journal of Analytieal Chemistry;1996-12
3 QI Li 1, LI Guang-ji 2 ( 1 Guangzhou Electric Apparent Research Institute,Guangzhou 510300,China; 2 Department of Polymer Scienee and Engineering, South China University of Technology, Guangzhou 510640,China);Properties and Applications of a Plant Polysacchride Konjac Glucomannan[J];Polymer Bulletin;2004-06
4 XIONG He, GAN Xin(Bio-technology institute, Hubei Poly-technology University, Wuhan 430068);Secretion and characteristis of R_(10) extracellular β-mannanase[J];Industrial Microbiology;2005-02
5 Meng Hai-lin, Zhang Yun-kai, Ling Min, Chen Gui-guang, Liang Zhi-qun (College of Life Science and Technology, Guangxi University,Nanning 530004, China);Breeding of β-mannanase Producing Strains[J];Modern Food Science and Technology;2006-02
6 Yang Yanan; Gao Shang; Wang Huiping; Xiong Yanfei; Ni hong; Ma Lixin(Faculty of Life Science, Hubei Univenity, Wuhan 40062, China);Studies on Preventive and Curative Effect of Konjac Oligosaccharides on Experimental Mice With Hyperlipidemia[J];JOURNAL OF HUBEI UNIVERSITY(NATURAL SCIENCE EDITION);1999-04
7 ZHANG Xue-wen1,2,TIAN Zhi-jian1,WU Yong-yao1,ZHOU Shuang-de2,KANG Dong-li1(1.College of Bioscience and Technology,HNAU,Changsha 410128,China;2.Institute of Agricultural Bio-Engineering,Changsha 410128,China);The Cloning and Expression of β-Mannanase Gene in E. coli[J];Journal of Hunan Agricultural University;2005-06
8 YANG Qing xing,LI Xue mei,LI Yong fang (College of Life Sciences,Henan Normal University,453002,Xinxiang,China);Study of the Characteristics of β-mannanase Produced by Alkalophilic Bacillus sp.NTT33[J];JOURNAL OF HENAN NORMAL UNIVERSITY(NATURAL SCIENCE);1999-03
9 YU Hongying1,3,SUN Yuanming1,WANG Weijun2,YANG Youhui1,YANG Yuesheng2(1. Collge of Food Science South China Agricultural University., Guangdong Guangzhou, 510642, China; 2. Colle of Life Sci., South China Agri. Univ; 3. Faculty of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangdong Guangzhou 510090, China);Studies on the Extraction of β-Mannanase by PEG/(NH_4)_2SO_4 Aqueous Two-phase System[J];Huaxue Shijie (Chemical World);2003-11
10 Liu Lanying, Cao Ya guang,Liu Baoqi, Li Qingshan,Wu Shanwei, Biao Jinqian, Wang Zongmu and Li Wei (Department of Molecular Biology, Jilin University);Studies on the Purification and Charactarization of Peptinase[J];Journal of Jilin University;1989-02
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved