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《Guangxi Agricultural Sciences》 2010-07
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Identification,isolation and cultivation of buffalo alveolar macrophage

HAN Zhong-yan,HUANG Hai-yu,SHEN Qian-cheng,ZHANG Wei-yu,HUANG Wei-yi,ZHONG Heng-lu(College of Animal Science and Technology,Guangxi University,Nanning 530005,China)  
The present experiment was conducted to establish the methodology of identification,separation,purification and cultivation of buffalo alveolar macrophage(AM) and to study the biological function of buffalo macrophage.Buffalo AM was isolated from lung-lavaging with cold PBS,the isolated cells were characterized functionally and phenotypically.The buffalo anchorage cells were obtained in large quantities which showed typical morphological characteristics of Mφ as observed by optical microscope and electron microscope.The positive rates of acid phosphatase and non-specific esterase were 89.4±3.5 and 83.6±3.4%,respectively.The positive rate of typical functional membrane proteins such as MHCⅡ was found to be 96.6±1.3%.The phagocytic rates of ink and chicken red blood cells were 92.3± 0.6 and 23.6 ± 4.2%,respectively.Therefore,the lung-lavaging with cold PBS was an efficient in vitro culture method for obtaining buffalo macrophage.
【Fund】: 广西自然科学基金项目(桂科自0832036)
【CateGory Index】: S823.83
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