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《Journal of Southern Agriculture》 2012-03
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Extraction of polyphenols from oil-tea(Camellia oleifera L.) seed cake using acetone solvent

XIE Yang-jiao1,XIE Dong-yang2,HE Zhi-peng1,YING Qi-shi 2,ZHOU Xiao-qing2,ZHONG De-pin2(1 Guangxi Botanical Garden of Medicinal Plants,Nanning 530023,China;2 Guilin Sanbao Pharmaceutical Co.,Ltd.,Guilin,Guangxi 546600,China)  
[Objective]This experiment was conducted to study the acetone extraction technology for extracting polyphenols from oil-tea(Camellia oleifera L.) seed cake to provide technical references for extracting,testing and exploiting polyphenol from oil-tea seed cake.[Method]Single factor experiment and orthogonal test design were used to extract polyphenols from oil-tea seed cake using acetone,following which the optimum extracting technique was discussed in terms of the following four elements:extraction time,extraction temperature,extract concentration and material/liquid ratio.[Result]Comprehensively considering the cost and extraction efficiency,extracting time of 0.5 h,extracting temperature of 50℃,extract concentration of 60% and material/liquid ratio of 1:15 were recorded as the best extracting condition to get the best extracting effects.[Conclusion]Extraction ratio of polyphenol attained(3.217±0.153)% by adopting the optimized acetone extracting technology,which was suitable to use in case of limited raw materials and highly pure products.
【Fund】: 广西科技攻关项目(桂科攻10123005-21);; 广西药用资源保护与遗传改良重点实验室科研项目(桂科基字[2010]8号)
【CateGory Index】: TQ914.1
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