Tissue culture and rapid propagation technologies for Scindapsus aureus
LIN Mao, WANG Hua-xin, TANG Qiu-ming, CHEN Bao-ling, DU Ling, YANG Shu-ting, GONG Jian-ying (Guangxi Forestry Research Institute, Nanning 530002, China)
[Objective]This research explored the tissue culture and rapid propagation of Scindapsus aureus in order to provide technical supports on green regeneration system establishment and factory production. [Method]The leaf and stem without buds of S. aureus were used as explants to investigate the effects of different plant hormone combinations(1.0-3.0 mg/L 6-BA, 0.1-0.3 mg/L NAA, 0.1-0.3 mg/L IBA) added to MS culture medium on callus induction, differentiation, adventitious bud, and rooting culture. [Result]The results showed that the explants could effectively induce the callus formation, but the stems induced callus and produced adventitious buds quickly. The suitable culture medium for leaves to induce callus was MS+6-BA 3.0 mg/L+NAA 0.3 mg/L, and the best culture medium of callus differentiation was MS+6-BA 2.0 mg/L+NAA 0.2 mg/L. For stem without buds, the best medium for callus induction and differentiation was MS+6-BA 2.0 mg/L+NAA 0.2 mg/L, and the optimum culture medium for rooting culture was 1/2MS+NAA 0.2 mg/L.[Conclusion]Leaf and stem without buds of Scindapsus aureus were better explants for callus culture, and stem show better culture effects than leaves.