Induction of resistance-related defensive enzymes in Hevea brasiliensis by Bacillus subtilis Czk1
TANG Wen;LIANG Yan-qiong;XU Pei-dong;ZHANG Chi-cheng;WU Wei-huai;ZHENG Xiao-lan;HE Chun-ping;YI Ke-xian;College of Environment and Plant Protection,Hainan University;Environment and Plant Protection Institute,Chinese Academy of Tropical Agriculture Sciences;Institute of Tropical Bioscience and Biotechnology;
【Objective】The effects of endophytic Bacillus subtilis Czk1 on activity of resistance-related defensive enzymes of Hevea brasiliensis plants were investigated to analyze induced disease-resistant mechanism of H. brasiliensis, in order to lay the foundation for application of B. subtilis Czk1 in biological prevention and control. 【Method】The activities of 5 defensive enzymes viz., catalase(CAT), superoxide dismutase(SOD), peroxidase(POD), phenylalanine(PAL) and polyphenol oxidase( PPO) were sprayed as disease- resistant indexes of H. brasiliensis plants. H. brasiliensis plants were sprayed with B. subtilis Czk1 fermentation liquor, its 10 times dilutent and 100 times dilutent, respectively, with H.brasiliensis plants sprayed with LB medium as control. And then the activities of 5 defensive enzymes were determined at different times. Moreover, the influences of anthracnose pathogen and B. subtilis Czk1 on activities of resistance-related defensive enzymes in H. brasiliensis plants were studied according to different orders of inoculated with anthracnose pathogen and B. subtilis Czk1. 【Result】The 10 times diluent of B. subtilis Czk1 showed optimal effect of induced resistance, because the CAT, POD, PAL and PPO activities in H. brasiliensis leaves were the highest, and the peak values of which were 4264.62, 28946.18, 186.67 and 53.70 U/g, respectively, and 5.24, 6.34, 3.19 and 3.38 times those of control group, respectively. However, the SOD activity in H. brasiliensis leaves treated with undiluted B. subtilis Czk1 fermentation liquor was the highest, its peak value was 1344.53 U/g, and 5.19 times that of control group. In addition, the H. brasiliensis leaves which were first sprayed with B. subtilis Czk1 fermentation liquor then inoculated with pathogen had significantly higher activities of CAT, SOD, POD, PAL and PPO than those of other treatment groups, the peak values of CAT, SOD, POD, PAL, PPO were 4129.02, 640.96, 7416.94, 173.35 and 71.59 U/g, respectively, and 4.20, 2.08, 2.50,2.56, and 7.64 times that of control group, respectively. 【Conclusion】Spraying B. subtilis Czk1 fermentation liquor can increase activities of resistance-related enzymes, so as to induce systemic resistance of H. brasiliensis plants. Furthermore, the induced disease resistance may be an important mechanism for using B. subtilis to control fungal diseases of H.brasiliensis.
【Fund】： 国家天然橡胶产业技术体系建设项目(nycytx-34-GW2-4-3 CARS-34-GW8);; 中央级公益性科研院所基本科研业务费专项项目(2014hzs1J013 2015hzs1J014)
【CateGory Index】： S763.7
【CateGory Index】： S763.7