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《JOURNAL OF GUANGXI NORMAL UNIVERSITY(NATURAL SCIENCE)》 1999-03
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TRANSFORMATION OF BACILLUS THURINGIENSIS CRYIB GENE B.CAMPESTRIS .L.SSP CHINENSIS (L.)MAKINA VAR.COMMANIS TSEN ET LEE

QIN Xin min GAO Cheng wei LI Chun yao DENG Zhi nian(Department of Biology,Guangxi Normal University,Guilin 541004 China)  
Plasmids pBYTESp101-1 and pBQX containing a modified insecticidal crystal protein gene CryIB are digested with Sph I and EcoR I,the modified CryIB gene on the pBQX is inserted into pBYTESp101—1 to replace the GUS gene and to get intermediate vector pBIWIQ—1.Then ,the pBIWIQ—1 is digested with Hind Ⅲ,the 5.1 kb fragment (mas-35S+CryIB+OCS 3') is inserted into the Hind Ⅲ site of pBIN19 to get a plant-expression vector with kanamycin resistant pBIWIQ-5.The pBIWIQ-5 is transferred into Agrobacterium EHA105by triparental mating method .Then the CryIB gene is transferred into B.campestris .L. ssp chinensis (L.)Makina var.commanis Tsen et Lee by Agrobacterium -mediated method.The results of PCR,Southern blot and Northern blot analysis indicate that the CryIB gene has been integrated and expressed in transgenic plants.
【Fund】: 广西教育厅科研资助项目
【CateGory Index】: Q785;S634.3
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