Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Guangxi Normal University(Natural Science Edition)》 2012-02
Add to Favorite Get Latest Update

Extraction of Total Triterpenoids from Ilex pubescens Hook leaves

YIN Wen-qing,FENG Hua-fen,DUAN Shao-qing,TAN Qiang,CEN Ning-cai(State Key Laboratory Cultivation Base for the Chemistry and Molecular Engineering of Medicinal Resources,College of Chemistry and Chemical Engineering,Guangxi Normal University,Guilin Guangxi 541004,China)  
The extraction process of triterpenoids from Ilex pubescens Hook leaves was investigated.The triterpenoids composition was determined by spectrumetric method with oleanolic acid as a reference.The influence of extracting times,radio of liquid to material,extracting time and concentration of ethanol on the triterpenoids of yield were evaluated by single factor experiments and orthogonal experiments.The results showed that the optimum conditions were 1∶16 in radio of liquid to material,concentration of ethanol 70%,extraction time 90 min and extracting two times.The content of triterpenoids was 6.18% in extract.
【Fund】: 广西自然科学基金创新团队项目(2010GXNSFF013001);; 药用资源化学与药物分子工程教育部重点实验室科研项目(07109001-03)
【CateGory Index】: TQ461
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 5 Hits
1 KUANG Chun-tao et al (College of Material Science and Engineering,Central South University of Forestry and Technology,Changsha,Hunan 410004);Study on Extraction Process of Total Flavonoids from Pubescent Holly Root[J];Journal of Anhui Agricultural Sciences;2009-01
2 YANG Yun-Yun1,GUO Peng-Ran1,CHEN Yi-Lu2,DENG Liu-Qin2,ZHONG Ming3,WU Qing-Hui11(Guangdong Key Laboratory of Chemical Emergency Test,China National Analytical Center,Guangzhou 510070)2(Guangzhou Children's Hospital,Guangzhou 510120)3(Guangzhou Hanfang Natural Medicine Research & Development Co.Ltd.,Guangzhou 510240);Rapid Identification of Triterpene and Saponin in Compound Radix Ilicis Pubescentis Medicinal Granules by High Performance Liquid Chromatography-Mass Spectrometry[J];Chinese Journal of Analytical Chemistry;2009-10
3 DING Ke1,2 CUI Ying2 LU Jingjing1 YUAN Qipeng1 1.Key Laboratory of Bioprocess of Beijing,Beijing University of Chemical Technology, Beijing 100029,China 2.Department of Food Science,Beijing University of Agriculture,Beijing 102206,China;STUDY ON PURIFICATION OF TOTAL TRITERPENOID SAPONINS IN Zizyphi spinosi semen BY USING SP700 MACROPOROUS RESIN[J];Ion Exchange and Adsorption;2011-01
4 ZHOU Zhong-liu1,ZOU Jie-ming2,WANG Li-sheng2,ZENG Li1,YIN Wen-qing1,2*(1.School of Chemistry & Chemical Engineering,Guangxi Normal University,Guilin 541004,China;2.Guilin Sanjin Pharmaceutical Co.Ltd.,Guilin 541004,China);Extraction,Purification and Characterization of Total Lignans from Ilex pubescens Hook.et Arn.[J];Lishizhen Medicine and Materia Medica Research;2008-04
5 YANG Yun-yun1,HUANG Shen-hui2,FENG Feng3,GUO Peng-ran1,CHEN Jian-xin2(1.Guangdong Key Laboratory of Chemical Emergency,China National Analytical Center(Guangzhou),Guangzhou 510070,China;2.Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation,College of Veterinary Medicine,South China Agricultural University,Guangzhou 510642,China;3.Department of Natural Medicinal Chemistry,China Pharmaceutical University,Nanjing 210009,China);Determination of Ilexgenin A and Ilexsaponin A_1 in Radix Ilicis Pubescentis by Accelerated Solvent Extraction Followed with HPLC-MS[J];Journal of Instrumental Analysis;2009-08
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 KUANG Chun-tao et al (College of Material Science and Engineering,Central South University of Forestry and Technology,Changsha,Hunan 410004);Study on Extraction Process of Total Flavonoids from Pubescent Holly Root[J];Journal of Anhui Agricultural Sciences;2009-01
2 LI Xiang-mei et al (The First Middle School in Xingtai City of Hebei Province,Xingtai,Hebei 054000);Comparison on Saponin Contents in Pods and Seeds of Bean[J];Journal of Anhui Agricultural Sciences;2009-17
3 YIN Wen-qing et al(Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources,Miinistry of Education of China,College of CHenistry and Chemical Enginering,Guangxi Normal University,Guilin,Guangxi 541004);GC-MS Analysis of Volatile Compoents from Leaves of Ilex pubescens Hook.et by Different Solvent[J];Journal of Anhui Agricultural Sciences;2011-20
4 SONG Yuan-yuan,LI Yuan,ZHANG Hong-quan(The Medical and Pharmaceutical Academy of Yangzhou University,Jiangsu Yangzhou 225001);Advances in studies on anti-inflammatory and immune actions of ilex pubescens hook.Et Arn.[J];Anhui Medical and Pharmaceutical Journal;2009-10
5 Ren Hong1 Zeng Youwei2 Xu Liangxiong3(1.Guangdong Boluo Pioneer Medicinal Group Co.,Ltd,HuiZhou 516100;2.Department of Food and Bio-engineering,Guangdong Light Industry Technical College,Guangzhou 510300,Guangdong,China;3.South China Botanical Garden,Guangzhou 510650);Advances in Studies on Triterpenoids Saponins and Their Bioactivity from the Root of Ilex Pubescens Hook.Et Arn[J];Journal of Guangdong Industry Technical College;2011-01
6 Zhang Luo-han,Liao Xiao-feng(Department of Applied Chemistry ECGI JX,Fuzhou 344000);Research Advance of New Functional Factor—Ursolic Acid in Foodstuff[J];Guangzhou Food Science and Technology;2004-03
7 LIN Ying-xing(Juncao Research Institute,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China);Study on the contents of polysaccharides and triterpenoids of Ganoderma lucidum at different growth stages[J];Subtropical Agriculture Research;2009-03
8 WANG Wen-juan;Northwest Institute of Plateau Biology,Chinese Academy of Sciences;;Total triterpenes in different parts of Hippophae rhamnoides with different ages in Qinghai Province[J];Pratacultural Science;2014-02
9 CHEN Yanjun1,2),CHENG Suxia1),LONG Yue1),HOU Qindong1) 1)Department of Chemistry,Zhengzhou University,Zhengzhou 450001 2) Department of Biology,Chemistry and Environment,Jiaozuo University,Jiaozuo 454150;Determination of the content of triterpenoidic acid in Leptopus Chinese (Bunge) Pojark[J];Journal of Zhengzhou University(Medical Sciences);2008-03
10 ZHOU Xin,XIE Rui-fang,GU Xi-jun(The Affiliated Longhua Hospital,Shanghai University of Traditional Chinese Medicine,Shanghai 200032);Determination of total tritepenoidic acid in injection Herba Hedyotis Diffusae[J];Journal of Hangzhou Teachers College(Medical Edition);2008-05
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 ZHU Zhi-yong et al(College of Chemistry and Bioengineering,Yichun College,Yichun,Jiangxi 336000);Content Determination of Ursolic Acid and Oleanolic Acid in Diospyros kaki by HPLC[J];Journal of Anhui Agricultural Sciences;2007-31
2 WANG Chang-lu et al(Laboratory of Food Biotechnology,College of Food Engineering and Biotechnology,Tianjin University of Science & Technology,Tianjin Key Laboratory of Food Nutrition and Safety,Tianjin 300457);Determination of Triterpene Saponins in Melia plants by Thin Layer Chromatography-spectrophotometry[J];Journal of Anhui Agricultural Sciences;2008-14
3 LI Meng(Institute for Drug Control of Rizhao,Rizhao,Shandong 276826);Determination of Oleanolic Acid in Fructus Ligustri Lucidi with HPLC Method[J];Journal of Anhui Agricultural Sciences;2008-24
4 HUANG Shu-ming~(1,4),YANG Xin-lin~1,HUANG Jian~1,XU Jian-lan~2,ZHU He-sun~3 (1.School of Life Science and Technology, Beijing Institute of Technology, Beijing100081, China; 2.Sanlian Hi-Tech Limited Company, Wuxi, Jiangsu214023, China; 3.School of Material Science and Engineering, Beijing Institute of Technology, Beijing100081, China; 4.Bengbu Technological Academy, Bengbu, Anhui233030, China);Method for the Rapid Quantification of Triterpenoids in Ganoderma Lucidum by Ultraviolet Spectrometry[J];Journal of Beijing Institute of Technology;2004-06
5 ZHOU Ben-hong1,2,WEI Yan2,ZHANG Chen-xia2,SHEN Heng2,QIU Zhen-peng2(1.Department of Pharmacy,Renmin Hospital of Wuhan University,Wuhan,Hubei 430060,China;2.College of Pharmacy,Wuhan University,Wuhan,Hubei 430072);Study on antioxidant activity of total tannins from Persimmon calyx[J];Journal of Guangdong Pharmaceutical College;2010-06
6 LING Qing-zhi1,2,GAO Li-li1,YUAN Huai-bo2,WEI Zhao-jun2(1.Zhejiang Pharmaceutical College,Ningbo,Zhejiang,315100,China;2.School of Biotecnology and Food Engineering,Hefei University of Technology,Hefei,Anhui, 230009,China);Study on Purification of Ligustrum lucidum Triterpenes with Macroreticular Resin[J];Guangxi Sciences;2009-04
7 HAN Zhihui,ZHANG Jingwei,ZHAO Yucong,LI Xinbao,LIU Guoji College of Chemical Engineering, Zhengzhou University, Zhengzhou 450001;Comparing the effect of seven extraction methods of the total saponins from Cornus officinalis sieb. et Zucc[J];Journal of Zhengzhou University(Science Medical);2005-03
8 LI Xian-cui, FAN Xiao, HAN Li-jun,et al.(Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071 ,China);Screening for α-Glucosidase Inhibitors from the Macroalgal Extracts[J];Chinese Journal of Marine Drugs;2002-02
9 ZHANG Hua-li,ZHU Jun,GAO Gui-hua(College of Pharmacy,Jining Medical University,Rizhao 276826,China);Determination of total triterpenes in Hypodematium sinense Iwatsuri[J];Journal of Jining Medical University;2011-03
10 CHEN Shangxing1,LIU Cheng2,FAN Guorong1, YAO Xujie1,JIANG Xiangmei3(1.College of Forestry,Jiangxi Agricultural University,Nanchang Jiangxi 330045,China;2.Anfu City Center for Agricultural Technique ,Anfu Jiangxi 343200,China;3.Jiangxi Academy of Forestry,Nanchang Jiangxi 330032,China);Techniques of Extraction and Separation and Analysis Methods for Active Components from Natural Products[J];Jiangxi Forestry Science and Technology;2005-03
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Wu Qi-lin Yuan Qi-peng Chen Yang-wen (College of Life Science and Technology,Beijing University of Chemical Technology,Beijing 100029, China);Preparation of cichoric acid by adsorption separation of macroporous resin[J];Journal of Beijing University of Chemical Technology;2004-05
2 GAN Lu 1, WANG Bin 1, LIANG Hong 1, ZHAO Yu Ying 1, JIANG Feng Chi 2 (1.Division of Natural Medicional Chemistry, School of Pharmaceutical Sciences, Peking University, Beijing 100083, China; 2.Department of Chemistry,Jiangxi Normal: Universi;Chemical constituents from Rubus alceaefolius Poir[J];JOURNAL OF BEIJING MEDICAL UNIVERSITY;2000-03
3 Li Meiqing 1, Sheng Xuan 2, Shao Xueguang *1 1(Department of Chemistry, University of Science and Technology of China, Hefei 230026) 2(Anhui Entry-Exit Inspection and Quarantine Bureau, Hefei 230061);Separation and Determination of Active Components of Pueraria Lobata Isoflavonoid Extract by Reversed Phase High Performance Liquid Chromatography[J];Chinese Journal of Analytical Chemistry;2003-02
4 Hou Dongyan *1,2 ,Hui Ruihua 1,2 , Mei Yang 2, Liu Xiaoyuan 3, Guan Chongxin 1 1(Department of Chemistry, Anshan Normal University , Anshan 114005) 2(Department of Chemistry, Liaoning Normal University, Dalian 116029) 3(Liaoning Longxing Biologe Science and Technology Co. Ltd., Anshan 114001);Determination of Total Flavonoids in Llex Kudincha by Three Wavelength Spectrophotometry[J];Chinese Journal of Analytical Chemistry;2004-06
5 Yin Jianyuan, Yang Xiaohong, Meng Qin,Wang Ensi* (College of Pharmacy,Jilin University,Changchun 130021);Structural Elucidation on a Triterpenic Heptaglycoside from Kalopanax Septemlobus[J];Chinese Journal of Analytical Chemistry;2004-10
6 ZHANG Min~*, CAO Yong, TANG Chun-yi, PENG Mi-jun, GONG Zhu-qong, LI Gui(Forest Products Chemical Engineering Key Laboratory of Hunan Province, Jishou University, Zhangjiajie, Hunan 427000);Determination of Total Flavanoid in Leaves of ginkgo biloba L. by Binary Complex Spectrofluorimetry[J];Journal of Analytical Science;2005-02
7 Zeng Lonmei and Su Jingyu(Department of Chemistry,Zhongshan Uniersity ,Guangzhou)Zhang Shude(Institute of Boiphysics,Academia Sinica,Beijing);Studies on the Chemical Structure of Ilexolide A (Ⅰ)[J];Chemical Research In Chinese Universities;1984-04
8 Huang Cai Liang Nianci (Department of Biochemistry);EFFECTS OF ILEX PUBESCENS INJECTION AND COMPOUND SALVIA MILTIORRHIZA INJECTION ON CYTOSKELETAL PROTEINS IN PIG PLATELETS[J];;1993-Z1
9 WU Fang-hui1,LI Wen-liang2,BIAN Ming-di2?穴1. Beijing Reseach Institute of Phormacenchemistry, 102205 Beijing,China;2. The Quartermaster University of PLA Departermant of Engineering, 130062 changchun,China);Research on the purification of soysaponins by AB-8 macroporous absorption resin[J];Hubei Agricultural Sciences;2004-03
10 YANG Peng-bo1,2, ZHANG Xiao-yan1, CONG Wei1, WANG Fang2, DENG Li2 (1. State Key Lab. Biochem. Eng., Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100080, China; 2. Key Lab. Bioprocess of Beijing, Col. Life Sci. & Technol., Beijing University of Chemical Technology, Beijing 100029, China);Adsorption and Separation of Lactic Acid and Glutamic Acid by Macroporous Resin Adsorbents[J];The Chinese Journal of Process Engineering;2007-04
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved