ENHANCED SENSITIVITY OF OVARIAN CANCER CELL LINE TO CISPLATIN INDUCED BY CURCUMIN AND ITS MECHANISM
Ying Huanchun,Lu Jing,Zhang Shulan,et al.(Department of Obstetrics and Gynecology ,Shengjing Hospital of China Medical University, Shenyang 110004 China)
Objective: To observe the effect of Curcumin in enhancing chemosensitivity of drug-resistant ovarian cancer cell line to cisplatin and investigate its mechanism. Methods: The growth inhibiting rates of COC1/ DDP cells by DDP, curcumin, DDP combined with curcumin were studied by methyl thiazolyl tetrazolium(MTT) method; the apoptic ratios of COC1/DDP cells by DDP, curcumin and DDP combined with curcumin were measured by flow cytometry; the expressions of apoptosis-associated proteins Bcl-2,Capase-3, Survivin were studied by Western blot in COC1/DDP cells. Result:15～35 μmol/L curcumin could significantly inhibit the growth of COC1/DDP cells, with the effect depending on concentration of curcumin; 1.25～5 mg/L DDP combined with 20 μmol/L curcumin could decrease surviving rates of COC1/DDP cells(P0.05), especially 2.5 mg/L DDP combined with 20 μmol/L of curcumin. The apoptic ratios of COC1/DDP cells by DDP combined with curcumin were significantly higher than those only by DDP or curcumin(P0.05). The expressions of Caspase-3 were distinctly higher in DDP combined with curcumin group than that only by DDP at protein level, but Bcl-2 and Survivin significantly lower(P0.05). Conclusion: Curcumin can inhibit the growth of COC1/DDP and enhance its sensitivity to DDP. Its mechanism may be associated with over expression protein of Caspase-3 and downregulation protein of Bcl-2 and Survivin.