Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Guangxi Medical University》 2010-01
Add to Favorite Get Latest Update

EFFECTS OF PLACENTA FACTOR ON THE GROWTH OF PC12 CELLS

Xu Yangxi,Yang Tiezheng,Zhang Xuerong,et al.(Institute of Snake Venom,Guangxi Medical University,Nanning 530021 China)  
Objective:To observe the effects of placenta factor on the growth of PC12 cells.Methods:The effect of PF on the survival rate of PC12 cells in serum-free medium and the proliferation and differentiation of PC12 cells in the low serum medium were determined by MTT test.The morphology changes of PC12 cells were observed by ordinary microscopic.Results:At the concentrations of 50 mg/L,25 mg/L,12.5 mg/L and 6.25 mg/L,PF could significantly increase the survival rate of PC12 cells in serum-free medium by different concentrations of PF for PC12 cells 44 hours (P0.05).At the concentrations of 12.5 mg/L and 6.25 mg/L,PF could significantly increase the number of PC12 cells in the low serum medium by different concentrations of PF for PC12 cells 68 hours(P0.01).During treating PC12 cells in the low serum medium with different concentrations of PF for 10 days,PC12 cells grew protuberance at the concentration of 3.13 mg/L and 1.56 mg/L of PF when PC12 cells had grown six days.Conclusion:The study showed that PF could enhance the survival rate of PC12 cells in serum-free medium,inhibit the proliferation of PC12 cells and induce the differentiation of PC12 cells.
【Fund】: 广西壮族自治区科技厅资助项目(No.桂科能0443001-17)
【CateGory Index】: R392
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 3 Hits
1 Qiang Liang, Gu Xingxing, Ding Fei (Nantong Medical College, Key Laboratory of Neuroregeneration of Jiangsu Province, Nantong 226001);NERVE REGENERATION FACTOR ACTIVATES ERK1/2 IN PC12 CELLS[J];Chinese Journal of Neuroanatomy;2003-04
2 Zang Jingtian, Ding Xiaohong, Li Xuanshe, et al(Department of Public Health Microbiology and Immunology);THE EFFECT OF ENHANCED PLACETA FACTOR(EPT) ON THE PRODUCTION OF CYTOKINES[J];Journal of Xi'an Medical University(Chinese);1995-03
3 Liu Yuexin et al The Air Force Hengyang Hospital, Hengyang Hunan;Preparation and Study on Placenta Factor——A New Immunomodulator[J];Chinese Journal of Immunology;1985-05
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 ZHONG Ying-ying (Southwest China Normal University, Chongqing 400715);Study on Inartificial Medicaments[J];;2005-02
2 ZHOU Ming-ming~(1,2),ZHANG Jun-fang~1,DING Fei~(1*) (1.The Key Laboratory of Neuroregeneration of Jiangsu Province,Nantong University,Nantong 226001,China;2.School of Life Science,Nantong University);Nerve regeneration factor promotes nerve regeneration after sciatic nerve injury in rats[J];Academic Journal of Second Military Medical University;2006-09
3 GE Rui-hong~1,JI Fang~2, ZHU Bei-wei~1, (1.Coll. of Bio. & Food Technol.,Dalian Inst. of Light Ind., Dalian 116034, China; 2. Medical Univ. of Dalian, Dalian 116030, China);Preparation of sheep placenta peptide[J];Journal of Dalian Institute of Light Industry;2003-04
4 Zheng Keqin, et al (Department of Biology and Genetics);Observations on the cytogenetic effect of immunoregulatory pcptide in placenta (IPIP) by short-term culture of human peripheral blood lymphocytes[J];Journal of Gannan Medical College;1991-01
5 Chen Shuiqing et al;The Effects of Frozen Human Placental Immunoregulatory Factor on Leukocyte Migration[J];Journal of Gannan Medical College;2001-03
6 WEN-Lijin~1,XIE Shui-xiang~2 (1.Urological department of Ganzhou ChangAn hospital;2.Department of Pathogenic biology,Gannan Medical University,Ganzhou Jiangxi 341000);Effects of TF on serum levels of IL-4,IL-6,TNF-α in patients with Genital herpes[J];Journal of Gannan Medical University;2006-06
7 Yan Yiming(central Laboratory, Gannan Medical College);Effects of human placental immunoregulatory factor on the RBC-C_(3b) receptor in the patients with schizophrenia[J];JOURNAL OF GANNAN MEDICAL COLLEGE;1994-02
8 CHEN Yi-ren~▲, TANG Xin, ZHANG Qi, DING Fei, GU Xiao-song . ~▲Key Laboratory of Neuroregeneration of Jiangsu Province, Nantong University,Nantong 226001, China;Changes in Gene Expressions Induced by Nerve Regeneration Factor in Cultured Hippocampal Neurons Detected with DNA Microarray[J];Neural Injury and Functional Reconstruction;2006-02
9 Zhang Xuerong;Shu Yuyan(The Snake Venom Research Institute of Guangxi Medical University,6 Binhu Road,Nanning Guangxi,530021);Experimental Study on Placenta Immunoregulatory Factor[J];GUANGXI SCIENCES;1996-04
10 Liang Jiheng, Zhang Lisheng, Zhang Litu, et al Affiliated Tumor Hospital of Guangxi Medical University, Guangxi Cancer Institute(Nanning 530021);Clinical study on 313 patients of malignant tumor treated with placental immunoregulating factor[J];GUANGXI MEDICAL JOURNAL;1999-01
China Proceedings of conference Full-text Database 1 Hits
1 Han Chunmei,Yu Lei,Fan Wenhong,Shi Xinchang,Rao Chunming (Department of Recombinant Technological Products,National Institute for the Control of Pharmaceutical and Biological Products of China,Beijing 100050);TF1-based cell proliferation assay for determining biological potency of mouse nerve growth factor[A];[C];2010
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Zhang Xuerong;Shu Yuyan(The Snake Venom Research Institute of Guangxi Medical University,6 Binhu Road,Nanning Guangxi,530021);Experimental Study on Placenta Immunoregulatory Factor[J];GUANGXI SCIENCES;1996-04
2 Dong Yuewei Xu Tianrui ** Xiong Yuliang Xu Kangsen *** (Yunnan Institute for Control of Drugs Kunming 650011);ESTABLISHMENT OF NEW QUALITATIVE AND QUANTITATIVE BIOASSAYS FOR NERVE GROWTH FACTOR USING PC12 PHEOCHROMOCYTOMA CELLS[J];WEST CHINA JOURNAL OF PHARMACEUTCAL SCIENCES;1999-Z1
3 Ma Buren, ei al. Department of Clinical Experiment, General Hospital, of Chengdu Command, PLA;AN EXPERIMENTAL STUDY ON IMMUNOLOGICAL ACTIVITY OF PLACENTAL PEPTIDE[J];Medical Journal of Chinese People's Liberation Army;1988-05
4 Zhu Yong Jin Boquan Sun Chen Liu Xuesong Zhao Ning Department of Immunology,The 4th Military Medical University, (Xi'an 710032)Chen Weifeng Department of Immunology,Beijing Medical University (Beijing 100083);MODIFICATION OF THE METHOD OF BIOASSAY OF IL-6 USING 7TD1 CELL LINES[J];;1992-02
5 Liu Mei, Gu Xiaosong, Liu Yan, Cui Xuezhi, Peng Yuping, Zhang Peiyun (Institute of Neuroscience, Nantong Medical College, Nantong 226001);THE PRELIMINARY STUDY OF GENE DIFFERENTIAL EXPRESSION DURING NERVE GROWTH WITH THE EFFECT OF "NERVE REGENERATION FACTOR[J];Chinese Journal of Neuroanatomy;2001-02
6 LI Yan - Lan;FENG Hui - Gen;REN Tai -Fang(Department of Cell Biology, Xinxiang Medical College; The First Affili ated Hospital of Xinxiang medical College);Effects of the placental immunoregulation polypeptide on immunone function in radiotherapy mice[J];JOURNAL OF XINXIANG MEDICAL COLLEGE;1997-03
7 FENG Hui-Gen(Department of Biology);Immunoregulatoy effects of placental immunoregulation polypeptide on mice with immunosuppression[J];JOURNAL OF XINXIANG MEDICAL COLLEGE;1995-02
8 PENG Li-Yi, YANGDao-Li, QI Fa-Lian, KE Bing-Shen, JIA Bo-Sen, ZHQNG Ren-Qian (Department of Immunology, General Hospital of Ji-nan Military Region, Ji-nan 250031);Identification and clinical study of placental immunoregulating polypeptides[J];;1990-03
9 CHEN Gang, WANG Xiao-Dong, ZHANG Pei-Yun, DING Fei Jiangsu Key Laboratory of Molecular Neurobiology, Nantong Medical College, Nantong 226001;Effect of Nerve Regeneration Factor on Apoptosis Cells in the Newborn Rat Spinal Cord[J];Journal of China Pharmaceutical University;2002-01
10 Jia Wanjun et al(Geriatric Department, 301 Hospital PLA, Beijing);Antisenility of Placenta Extract:A observation of Immunological Indices[J];;1988-04
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved