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《Guihaia》 2002-06
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Extraction and determination of DNA from Heptacodium miconioides

LI Jun-min, KE Shi-sheng, JIN Ze-xin ( Department of Biology and Chemistry, Taizhou Teachers` College, Linhai 317000, China )  
DNA was extracted from tender leaves of Heptacodium miconioides by using different methods. The quality was determined by UV spectrometers and the quantity was doubly determined by electrophoresis and spectrometers. The best methods for the DNA extraction of Heptacodium miconioides was improved SDS method,which was suitable for PCR analysis. DNA was extracted from different organs of Heptacodium miconioides using this method. It showed that the maximal DNA content was in tender leaves,and followed by older leaves,twig,older stem,side-bud.
【Fund】: 浙江省自然科学基金项目 (3992 0 3);; 浙江省教委科研计划项目 (1990 36 7)。
【CateGory Index】: Q943.2
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【References】
Chinese Journal Full-text Database 10 Hits
1 LI Jun-min,JIN Ze-xin(Ecology Institute of Taizhou University,Linhai 317000);Optimization and screening of ISSR reaction system in endangered plant Emmenopterys henryi[J];Journal of Anhui Agricultural University;2006-04
2 DING Li-ya~(1,2),JIN Zen-xin~2,LI Jun-min~2(1.Beijing Forestry University College of Natural Resources and Enviroment,Beijing 100083,China;2.Ecology Institute of Taizhou University,Zhejiang Linhai 317000,China);Optimization of the ISSR-PCR reaction system of Quercus glandulifera var.brevipetiolata[J];Journal of Fujian Forestry Science and Technology;2006-01
3 BI Quan-xin1,JIN Ze-xin1,ZENG Zhi-cheng1,YE Wen-guo2,LI Jian-hui1 (1.Ecological Research Institute of Taizhou College,Linhai Zhejiang 317000,China;2.Tiantai County Forestry Bureau,Tiantai,Zhajiang 317200,China);The optimization of ISSR-PCR conditions of Liquidambar formosana[J];Journal of Fujian Forestry Science and Technology;2008-01
4 SONG Wen-jing1,2,JIN Ze-xin2,LI Jun-min2,LI Jian-hui2(1.College of Life and Environmental Science,Hangzhou Normal University,Hangzhou 310036,Zhejiang,China;2.Institute of Ecology,Taizhou College,Linhai 317000,Zhejiang,China);Optimization of ISSR-PCR System of Fagus hayatae[J];Journal of Fujian Forestry Science and Technology;2009-02
5 GU Qi-Ping1,2,JIN Ze-Xin2,LI Jun-Min2(1.Key Laboratory of the Three Gorge Reservoir Region's Eco-Environment,Ministry of Education,Southwest University,Chongqing 400715,China;2.Institute of Ecology,Taizhou University,Linhai 317000,China);Optimization of ISSR amplification conditions for Rhododendron fortunei[J];Guihaia;2007-04
6 PANG Yu-Xin~(1,2),WANG Wen-Quan~(1*),ZHANG Ying-Bo~(2,3), LIU Guo-Dao~2,MO Ting-Hui~3 (1.College of Chinese Medical Sciences,Beijing University of Traditional Chinese Medicine,Beijing 100102,China; 2.China Tropical Crops Genetic Resources Institute of Chinese Academy of Tropical Agricultural Sciences, Danzhou 571737,China;3.College of Agriculture,Hainan University,Danzhou,571737,China);Method of genomic DNA extraction of the herb Blumea balsamifera[J];Guihaia;2009-06
7 LI Jian-hui1,2,FENG Yong-hui3,YAO Yong-bin4 (1.College of Life and Environment Sciences,Hangzhou Normal University,Hangzhou 310036,China;2.Ecology Institute of Taizhou University,Linhai 317000,China;3.Dazhan Middle School,Xianju 317321,China;4.Hengxi Middle School,Xianju 317312,China);Establishment and Optimization of ISSR Reaction System for Pittosporumtobira[J];Journal of Hangzhou Teachers College (Natural Sciences Edition);2007-04
8 JIN Ze-xin, LI Jun-min(Department of Biology, Taizhou University, Linhai 317000, China);Analysis of the genetic diversity of Hepatacodium miconioides at different altitude in Tiantai mountain in Zhejiang Province and its relationship with environmental factors[J];Journal of Zhejiang University(Sciences Edition);2005-04
9 JIN Ze-xin,LI Jun-min,PAN Guan-qiong(Institute of Ecology,Taizhou University,Linhai 317000,China);Primary study on genetic variation and genetic diversity among Castanopsis eyrei populations based on inter-simple sequence repeat(ISSR) markers[J];Journal of Zhejiang University(Science Edition);2007-06
10 LI Jian-hui,JIN Ze-xin,LI Jun-min(Institute of Ecology,Taizhou University,Linhai 317000,Zhejiang Province,China);RAPD and ISSR analysis on genetic diversity of different life stages in the population of Torreya jackii,an endangered plant in China[J];Journal of Zhejiang University(Science Edition);2010-01
【Citations】
Chinese Journal Full-text Database 2 Hits
1 Yin TongmingHan ZhengminHuang MinrenWang MingxiuLi Shuxian (Forest Genetics and Gene Engineering Laboratory Nanjing Forestry UniversityNanjing210037);RAPD Experiment Protocol and Optimization in Forest Trees[J];JOURNAL OF NANJING FORESTRY UNVERSITY;1999-04
2 Liu Kangde; Li Jianguo; Peng Shiqin; Zheng Xueqin(Tropical Horticulture Institute, CATAS, Danzhou, Hainan, 571737; National Key Biotechnology Laboratory for Tropical Crops, CATAS, Haikou, Hainan, 571101);Genome DNA Extraction and RAPD Analysis of Avocado[J];CHINESE JOURNAL OF TROPICAL CROPS;1999-04
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 LI Jian-hui et al (College of Life Science,Hangzhou Teachers College,Hangzhou,Zhejiang 310018);Establishment and Optimization of ISSR Reaction System of Schima superba[J];Journal of Anhui Agricultural Sciences;2006-19
2 QUE Sheng-quan et al(Department of Biology and Environmental Engineering,Nanchang University of Technology,Nanchang,Jiangxi 330013);DNA Extraction from Camellia oleifera and the Establishment of the Optimum Reaction System of RAPD Analysis[J];Journal of Anhui Agricultural Sciences;2008-03
3 XU Jian-peng et al(College of Life Science,Ludong University,Yantai,Shandong 264025);DNA Extraction from Kareius bicoloratus Genome and Its Optimization of RAPD Reaction System[J];Journal of Anhui Agricultural Sciences;2008-35
4 MAO Yun-ling et al (Yunnan Academy of Forestry,Yunnan Key Laboratory for the Protection and Propagation of Rare and Endangered Forest Plants of State Forestry Bureau,Key Laboratory for the Cultivation and Exploitation of Forest Plants in Yunnan Province,Kunming,Yunnan 650204);Establishment of ISSR-PCR Reaction System for the Rare and Endangered Plant Manglietia grandis Hu et Cheng[J];Journal of Anhui Agricultural Sciences;2009-31
5 CHEN Lei,CAO Hou-nan,ZONG Chen-wen,PIAO Ri-zi,XIAO Jia-li (Agricultural College of Yanbian University,Horticulture Department,Longjing,Jilin 133400,China);DNA Extraction on Prunus and Optimal Reaction System of RAPD[J];Northern Horticulture;2008-08
6 ZHU Xiu-zhi~1,XIANG Cheng-hua~2,PENG Zheng-song~1,YANG Jun-bao~1,LUO Cheng-ke~3 (1.College of Life Science,China West Normal University,Nanchong 637002,China;2.Sichuan Academy of Forestry,Chengdu 610000,China;3.Ningxia University,Yinchuan 750021,China);Genomic DNA extraction and optimization of RAPD analytic conditions of Michelia wilsonii[J];Journal of Agricultural University of Hebei;2006-02
7 Jiang Yuanbiao(Forestry Research Institute of Simao Prefecture,Yunnan Province,Simao 665000,P.R.China);Wu Tao,Chen Shaoyu,Yuan Ruiling(the Key Laboratory of Forest Plant Cultivation and Utilization of Yunnan Province,Yunnan Academy of Forestry);Optimization of RAPD Reaction Conditions for Pinus kesiya var. langbianensis[J];Journal of Northeast Forestry University;2007-09
8 PAN Xiao-hua,HUANG Ru-zhu,ZHU Jin-mao(Bioengineering College,Fujian Normal University,Fuzhou 350007,China);Study on Total DNA Extraction from Cinnamomun Camphora[J];Journal of Fujian Forestry Science and Technology;2004-01
9 DING Li-ya~(1,2),JIN Zen-xin~2,LI Jun-min~2(1.Beijing Forestry University College of Natural Resources and Enviroment,Beijing 100083,China;2.Ecology Institute of Taizhou University,Zhejiang Linhai 317000,China);Optimization of the ISSR-PCR reaction system of Quercus glandulifera var.brevipetiolata[J];Journal of Fujian Forestry Science and Technology;2006-01
10 CHEN Gui xin 1, PAN Dong ming 1, LU Liu xin 1, WANG Feng hua 2, LAI Zhong xiong 2 (1.College of Horticulture;2.Institute of Subtropical Fruits, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China);Advances in the techniques for extraction of nuclear DNA and isolation and cloning of target genes in fruit trees[J];Journal of Fujian Agricultural University(Natural Science);2002-01
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 LI Jian-hui et al (College of Life Science,Hangzhou Teachers College,Hangzhou,Zhejiang 310018);Establishment and Optimization of ISSR Reaction System of Schima superba[J];Journal of Anhui Agricultural Sciences;2006-19
2 WANG Guang-lin~(1,2),WANG Li-long~1,SHEN Zhang-jun~1,LIU Deng-yi~1(1.College of Life Science, Anhui Normal University, Wuhu 241000,China;2.Department of Biology,West Anhui University,Lu'an 237000,China);STUDY ON RELATION OF HEAVY METAL POLLUTION IN SOILS OF RICE LANDS NEAR SMELTERY AND ENZYME ACTIVITIES[J];Journal of Anhui Normal University(Natural Science);2004-03
3 JIN Ze-xin~1,LI Jun-min~1,ZHU Xiao-yan~2(1.Institute of Ecology,Taizhou University,Linhai 317000;2.Chengfeng Middle School in Xianjun County,Xianju 317300);Analysis of the total phenols content in different organs of Calycanthus chinensis from different habitat[J];Journal of Anhui Agricultural University;2006-04
4 LI Jun-min,JIN Ze-xin(Ecology Institute of Taizhou University,Linhai 317000);Optimization and screening of ISSR reaction system in endangered plant Emmenopterys henryi[J];Journal of Anhui Agricultural University;2006-04
5 FENG Wei-Song ① YANG Jun ① YE Zhi-Hong ② MIAO Wei ① YU Yu-He ① HUANG Ming-Hong ② SHEN Yun-Fen ①(①Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 43007 2; ②Institute for Natural Resource and Environmental Management, and Department of Biology, Hong Kong Baptist University,China);Soil Protozoa in Wetland Treatment System of Pb-Zn Mine in Fankou[J];Chinese Journal of Zoology;2004-01
6 Liu Peng; Xu Gendi; Yang Weiwei. College of Life and Environment Science, Zhejiang Normal Univ., 321004, P.R.China.;Effects of molybdenum on physiological characteristics of Heptacodium miconioides .[J];Journal of Beijing Forestry University;2002-02
7 Zhang Li; Zheng Youliang; Wei Yuming; Liu Shigui; Zhou Yonghong. Lab. of Bio control of Grassland, Sichuan University, Chengdu, 610064, P. R. China.;Genetic variation in Kengyilia populations based on Randomly Amplified Polymorphic DNA (RAPD) analysis.[J];Journal of Beijing Forestry University;2003-03
8 JIN Ze-xin;LI Jun-min;LI Zeng-hong.Institute of Ecology,Taizhou University,Linhai City,Zhejiang Province,317000,P.R.China.;ISSR analysis of the genetic diversity of Torreya jackii Chun natural populations in Xianju County,Zhejiang Province.[J];Journal of Beijing Forestry University;2007-01
9 Zhang Ruohui(Zhejiang Forestry College,Lin'an,311300,P.R.China) Shen Xianglin(Forestry Administration of Huzhou,Zhejiang,313000,P.R.China);Taxonomy Geographical Distribution and Evolution of Calycanthaceae[J];JOURNAL OF BEIJING FORESTRY UNIVERSITY;1999-02
10 Liu Hong ,e(Zhejiang Forestry College,Lin'an,Zhejiang,311300,P.R.China) Xu Yaoliang(Zhejiang Natural Museum,Hangzhou,310012,P.R.China) Yang Fengchun(Tianmushan Nature Reserve,Lin'an,Zhejiang,311311,P.R.China);The Flowering Season and Pollination Agent of Members Belonging to Calycanthaceae[J];JOURNAL OF BEIJING FORESTRY UNIVERSITY;1999-02
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 WANG Ji-zhen,LIU Ya-qiong,YANG Bing,WANG Xian*(College of Forestry and Horticulture,Henan Agricultural University,Zhenzhou 450002,China);ISSR Molecular Marker and Its Application in Garden Plant Genetics and Breeding[J];Shandong Agricultural Sciences;2008-08
2 ZHAO Wei et al (College of Life Science and Technology,University of Electronic Science and Technology,Chengdu,Sichuan 610054);An Effective Method of DNA Extraction from Crassulaceae Plant[J];Journal of Anhui Agricultural Sciences;2006-22
3 LI Rui-huan et al(College of Forestry,Northwest A&F University,Yangling,Shaanxi 712100);Genomic DNA Extraction and RAPD System Optimization of Chinese Jujube[J];Journal of Anhui Agricultural Sciences;2007-17
4 LUO Yue-ji et al(Institute of Life science and Biotechnology,Bioenergy and Biomaterial Research Center,Hunan University,Changsha,Hunan 410082);Establishment and Optimization of ISSR-PCR Reaction System and Genome DNA of Discolor cinquefoil Herb[J];Journal of Anhui Agricultural Sciences;2008-03
5 BAI Yi et al(College of Life Sciences,Taizhou University,Linhai,Zhejiang 317000);Analysis of the Characteristics of Animals in Soil Grown with Heptacodium miconioides Forest in Tiantai Mountain of Zhejiang and its Dissimilarity with Different Plantations[J];Journal of Anhui Agricultural Sciences;2008-12
6 TANG Xiao-chuang et al (Pharmacy College,Wenzhou Medical College,Wenzhou,Zhejiang 325035);Establishment of ISSR-PCR Reaction System for Curcuma wenyujin and Its Conditions Optimization[J];Journal of Anhui Agricultural Sciences;2008-22
7 WANG Wen-feng et al(College of Forestry Science,Gansu Agricultural University,Lanzhou,Gansu 730070);Establishment and Optimization of ISSR-PCR Reaction System for Picea crassifolia kom.[J];Journal of Anhui Agricultural Sciences;2009-21
8 MA Xue-mei et al(School of Biotechnology and Food Engineering,Anyang Institute of Technology,Anyang,Henan 455000);Advance in Studies on Chimonanthus praecox[J];Journal of Anhui Agricultural Sciences;2010-34
9 WU Juan,FANG Hui-ling,CAI Bing(School of Life Science,Anqing Teachers College,Anqing 246011,China);Polyacrylamide Gel Electrophorus Used in Rice SSR Marker[J];Journal of Anqing Teachers College(Natural Science Edition);2010-03
10 LU Yi-zeng(Shandong Forestry Seedling Breeding Center,Jinan,Shandong 250014);Discussion on the Taxonomic Status of Camellia chekiangoleosa[J];Northern Horticulture;2010-02
China Proceedings of conference Full-text Database 3 Hits
1 Yao Lei Wu Yani Le Yunchen Ye Lanrong ZhangYanling (School of Agriculture and Biology,Shanghai Jiaotong University,Shanghai 201101);Analysis of Heredity Relations and Comparison of Botanical Characters and Essential Oil Composition between Varieties of Mentha[A];[C];2008
2 ZHENG Yu-shan,HUANG Yu,RONG Jun-dong,CHEN Li-guang (Institute of Industrial Forest,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China);Establishment and optimization of ISSR reaction system of Sarcandra glabra[A];[C];2009
3 ZHENG Yu-shan,HUANG Yu,RONG Jun-dong,CHEN Li-guang (Institute of Industrial Forest,Fujian Agriculture and Forestry University,Fuzhou, Fujian 350002,China);Establishment and optimization of ISSR reaction system of Sarcandra glabra[A];[C];2009
【Secondary Citations】
Chinese Journal Full-text Database 4 Hits
1 Xiao Shunyuan and Zhang Wencai(Citrus Research Institute, Huazhong Agricultural University, Wuhan, 430070);Study on the Application of RFLP Markers in Assessing Citrus Genetic Diversity[J];;1995-01
2 Xu Biyu; Jin Zhiqiang; Peng Shiqing; Xu shupei; Zheng Xueqin (1Dept. of Hort,SCUTA,Danzhou,Hainan 571737; 2National Key Biotechnology Laboratory for Tropical Crops,CATAS,Haikou 571101);RAPD Analysis of Genomic DNA in Mango Cultivars in Hainan Island[J];CHINESE JOURNAL OF TROPICAL CROPS;1998-03
3 Xinlu Chen;Zhao Xiangyun;Brodley N.Whlte2,and James S.Pringle3(Department of Landscape&Architecture,Beijing Agricultural College 102208;Department of Biology,McMaster University,Hamilton. Ontario,Canada L8s 4Kl;The Royal Botanical Gardens,P.O.Box399,Hamil;Analysis of Genetic Relationship among Lilacs(Syringa)by RAPD[J];;1995-02
4 Chen Daming; Zhang Shanglong ; Jin Yongfeng (Department of Horticulture, Zhejiang Agricultural University, Hangzhou 310029, China);A method for genomic DNA preparation of woody fruit crops[J];Journal of Zhejiang Agricultural University;1997-06
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