Developing RAPD system for Stylosanthes and genetic diversity analysis of eight varieties
JIANG Chang-shun~(1,2), GE Qin-ya~1, ZOU Dong-mei~2, LIANG Ying-cai~3, ZHANG Yi-zheng~1 ( 1.College of Life Sciences, Sichuan University, Chengdu 610064, China; 2.The Institute of Tropical Crop Germplasm, Chinese Academy of Tropical Agricultural Science, Danzhou 571737, China; 3.Guangxi Institute of Animal Science, Nanning 530001, China )
High quality DNA was extracted from the young leaves and seedlings of Stylosanthes with two different methods,respectively. The RAPD reaction system for Stylosanthes was developed after optimizing the concentrations of Mg~(2+),dNTPs,Taq DNA polymerase and template DNA. Eight varieties of Stylosanthes spp. were analyzed by RAPD for genetic diversity. RAPD analysis was performed using twenty primers,and a 51.9% level of polymorphism was found. The dendrogram was constructed with UPGMA(unweighted pair group method of averages)based on the RAPD data,and it showed genetic similarity from 53% to 88% among varieties.