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《Guihaia》 2005-01
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Cloning and expression vector construction of Arabidopsis NPR1 gene

QIN Xin-min, LI Wen-lan, ZHANG Li-zhen, LI Hui-min, QIN Ping-sheng( College of life science, Guangxi Normal University, Guilin 541004, China )  
The Arabidopsis NPR1 gene plays an important role in activating various plant defense responses,including expression of the pathogenesis-related genes and systemic acquired resistance. The NPR1 gene was amplified from Arabidopsis thaliana genome DNA by DNA-PCR method. The DNA sequenced analysis showed that the sequence of amplified NPR1 gene was the same as the published sequence. Therefore, the plant expression vector pCaMVNPR containing NPR1 gene was constructed. The experiment will provide avenues for NPR1 gene application in plant disease resistant genetic engineering.
【Fund】: 广西科技攻关项目(桂科攻0228019 2)
【CateGory Index】: S336
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