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《Guihaia》 2006-05
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Cloning and characterization of atpA gene frag-ment from the chloroplast of Dunaliella salina

HOU Gui-qin 1,2 , LIU Hong-tao1, PAN Wei-dong1, XUE Le-xun 1,2* , JIANG Dong-ya3 ( 1.Laboratory for Cell Biology, Zhengzhou University, Zhengzhou 450052, China; 2.The First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, China; 3.The First High School of Xin’an County, Luoyang 471000, China )  
According to conserved motif of the homologous amino acid sequence of ten kinds of algae,a pair of degenerate primers were designed and about 400bp fragment was amplified from the chloroplast of Dunaliella salina by PCR technique. The resulting PCR products were inserted into pMD-18 T-vector and sequenced. The results showed that the nucleotide sequence was 405bp which encodes 135 amino acid,and the sequence shared high homology with atpA,with identity being 92%,88%,87%,86% and 85% to Chlamydomonas reinhardtii,Chlorella vulgaris,Mesostigma viride,Nephroselmis olivacea and Cyanidioschyzon merolae,respectively. The cloned fragment was used as a probe,which would be hybridized with chloroplast DNA of D.salina by Southern Blotting. Southern Blotting result revealed that there were obvious hybridization signals on cpDNA of D.salina. It can be concluded that the cloned sequence is atpA gene fragment from the chloroplast of D.salina. The atpA gene was accepted by GenBank(Accession number:AY435096).
【Fund】: 国家自然科学基金(30300208);; 河南省医学科技创新人才工程项目(20020021)~~
【CateGory Index】: Q943.2
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