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《Guihaia》 2007-01
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Tissue culture and rapid proliferation of Morinda of ficinalis How

HUANG Ning-Zhen,FU Chuan-Ming,ZHAO Zhi-Guo,TANG Feng-Luan,LI Feng(Guangxi Institute of Botany,Guangxi Zhuangzu Autonomous Region and the Chinese Academy of Sciences,Guilin 541006,China)  
The tissue culture and rapid proliferation techniques of Morinda officinalis How were studied by using apical buds and tender stems as explants.The explants were cultivated in different MS medium with different types and concentrations of plant hormones.The results showed that the most suitable procedure for sterilization was to soak explants into 0.1% HgCl2 solution for 10 minutes after pretreatment with 70% alcohol for 60 seconds.The tender stems were superior to apical buds in vitro culture procedure.The medium MS+IBA 0.05 mg/L+BA 1.0 mg/L was effective to induce shooting and suitable for the first generation cultivation;MS+IBA 0.2 mg/L+BA 1.0 mg/L could effectively induce fasciculate buds and be suitable for subculture and proliferation,the propagation coefficient was 6.0 every 50 days;the most optimum rooting medium was 1/2MS+IBA 0.4~0.8 mg/L,and the rooting rate was 100%.The rooting seedlings were transplanted in sand or burnt soil with plant ash.The highest survival rate was 90%.
【Fund】: 广西科技攻关项目(桂科攻0322024-3B)~~
【CateGory Index】: S567.239
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