Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Guihaia》 2007-04
Add to Favorite Get Latest Update

Optimization of ISSR amplification conditions for Rhododendron fortunei

GU Qi-Ping1,2,JIN Ze-Xin2,LI Jun-Min2(1.Key Laboratory of the Three Gorge Reservoir Region's Eco-Environment,Ministry of Education,Southwest University,Chongqing 400715,China;2.Institute of Ecology,Taizhou University,Linhai 317000,China)  
Factors which affect the ISSR-PCR amplification,such as Mg2+concentration,dNTP concentration,template DNA dosage,Taq DNA polymerase units,BSA concentration and primer dosage,and annealing temperature were optimized and selected by using the genomic DNA of Rhododendron fortunei as material.The suitable ISSR-PCR conditions BSA,12 pmol primer,16 ng template DNA.The suitable annealing temperature was 56.9 ℃.Based on these suitable conditions,12 primers were selected and 170 DNA bands were produced in 5 populations with 100 individuals among which 150 loci were polymorphic.The total percentage of polymorphic loci was 88.24%,while the mean percentage of polymorphic loci of 5 Rh.fortunei population was 48.23%.The establishment of the PCR reaction conditions could settle favorable basis for the further study on the genetic diversity of Rh.fortunei by using ISSR molecular marker techniques.
【Fund】: 浙江省自然科学基金(Y504220);; 台州市科技局项目(044205)~~
【CateGory Index】: Q943.2;S685.21
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 4 Hits
1 HU Yan-Ping1,2,XIE Xiao-Long1,2,WANG Li1,YANG Jian1,2,LI Yi1( 1.Key Laboratory of Adaptation and Evolution of Plateau Biota,Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Xining 810001,China; 2.Graduate University of Chinese Academy of Sciences,Beijing 100049,China );Establishment and optimization of ISSR-PCR reaction conditions for Rheum tanguticum[J];Guihaia;2010-01
2 HAO Chao-yun, ZHANG Xiao-ping, FAN Rui (Anhui Normal University a. Provincial Key Laboratory of Conservation and Employment of Biological Resources; b. Key Laboratory of Biotic Environment and Ecological Safety in Anhui Province, Wuhu 241000, Anhui, China);Establishment and Optimization of ISSR-PCR Reaction System for Monimopetalum chinense[J];Acta Laser Biology Sinica;2008-05
3 Tang Yanqiong1,3 Wu Ziyun1 Guo Jianchun2 Hu Xinwen1 Liu Guodao3 1 College of Agronomy, Hainan University Danzhou Hainan 571737 China 2 Institute of Biotechnology and Bioscience, CATAS Haikou 571101 China 3 nstitute of Tropical Crops Genetic Resources, CATAS Danzhou Hainan 571737 China;DNA Extraction and Optimization of ISSR-PCR System with Orthogonal Design for Stylosanthes Spp.[J];Chinese Journal of Tropical Crops;2008-03
4 LIU He-ping1,2,HE Ye-hua1,HU Gui-bing1,LIN Shun-quan1,HUANG Jian-feng1,XIE Zhi-liang1,LIN Jian-bo2 (1.College of Horticulture,South China Agricultural University,Guangzhou 510642,Guangdong,China;2.Yangjiang Vocational and Technical College,Yangjiang 529566,Guangdong,China);Optimization and application of inter simple sequence repeat reaction system of Dracaena fragrans[J];Journal of Central South University of Forestry & Technology;2010-04
【Citations】
Chinese Journal Full-text Database 3 Hits
1 HE Qiao, LIANG Guo-lu, XIE Jiang-hui (College of Horticulture and Landscape , Southwest Agricultural University, Beibei, Chongqing 400716 China;Southern Subtropical Crop Research Institute , Chinese Academy of Tropical Agricultural Sciences ,Zhanjiang, Guangdong 524091 China);Optimization of the inter-simple sequence repeat reaction system and its preliminary application in wax apple(Syzygium samarangens)[J];Journal of Fruit Science;2005-02
2 LI Jun-min, KE Shi-sheng, JIN Ze-xin ( Department of Biology and Chemistry, Taizhou Teachers` College, Linhai 317000, China );Extraction and determination of DNA from Heptacodium miconioides[J];Guihaia;2002-06
3 XI Jia-bin, ZHENG Yu-zhong,YANG Zhong-yi (School of Life Sciences, Sun Yat-sen University, Guangzhou 510275,China);Establishment and Optimization of ISSR Reaction System for Carpetgrass[J];Acta Scientiarum Naturalium Universitatis Sunyatseni;2004-03
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 LUO Yue-ji et al(Institute of Life science and Biotechnology,Bioenergy and Biomaterial Research Center,Hunan University,Changsha,Hunan 410082);Establishment and Optimization of ISSR-PCR Reaction System and Genome DNA of Discolor cinquefoil Herb[J];Journal of Anhui Agricultural Sciences;2008-03
2 LI Jun-min,JIN Ze-xin(Ecology Institute of Taizhou University,Linhai 317000);Optimization and screening of ISSR reaction system in endangered plant Emmenopterys henryi[J];Journal of Anhui Agricultural University;2006-04
3 FENG Xia-lian~1;HE Cheng-zhong~1;ZHANG Zhi-yi~1;AN Xin-min~1;YANG Kai~2;ZHANG You-hui~3.1 Key Laboratory for Genetic and Breeding in Forest Trees and Ornamental Plants, Ministry of Education,Beijing Forestry University,100083,P.R.China;2 Department of Biotechnology,Beijing Agricultural College,102206, P.R.China;3 Guanxian County Tree Nursery,Shandong Province,252528,P.R.China.;Establishment and optimization of an ISSR reaction system for Populus tomentosa Carr.[J];Journal of Beijing Forestry University;2006-03
4 WANG Kang1,2,DONG Kuan-hu2,SUN Yan3,KANG Jun-mei1*,DONG Jie2,MENG Lin4(1.Institute of Animal Sciences,CAAS,Beijing 100193,China;2.School of Animal Science,Shanxi Agricultural University,Taigu,Shanxi 030801,China;3.Institute of Grassland Science,China Agricultural University,Beijing 100193,China;4.Beijing Research Development Center for Grass and Environment,BAAFSR Beijing 100097,China);Establishment and Optimization of ISSR-PCR Reaction System for Iris lacteal[J];Acta Agrestia Sinica;2008-06
5 WANG Fang1,YUAN Qing-hua1,2*(1.College of Pastoral Agriculture Science and Technology,Lanzhou University,Lanzhou,Gansu Province 730020,China;2.Insfitute of Animal Science,CAAS,Beijing 100193,china);Establishment and Optimization of ISSR Reaction System for Agropyron cristatum(L.) Gaertn.[J];Acta Agrestia Sinica;2009-03
6 FAN Yan1,2,LI Fang1,ZHANG Xin-quan1,MA Xiao1(1.Department of Grassland Science,Sichuan Agricultural University,Ya'an 625014,China;2.Chongqing Municipal Institute of Animal Husbandry,Chongqing 400039,China);Genetic diversity of Hemarthria compressa germplasm detected by inter-simple sequence repeat(ISSR)[J];Acta Prataculturae Sinica;2007-04
7 HUANG Yu,CHEN Li-guang,XIA Hai-tao,RONG Jun-dong,KONG Ling,LIAO Peng-hui,ZHENG Yu-shan(Institute of Industrial Forest,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China);Establishment and optimization of ISSR-PCR reaction system of Michelia fujianensis[J];Journal of Fujian College of Forestry;2009-02
8 DING Li-ya~(1,2),JIN Zen-xin~2,LI Jun-min~2(1.Beijing Forestry University College of Natural Resources and Enviroment,Beijing 100083,China;2.Ecology Institute of Taizhou University,Zhejiang Linhai 317000,China);Optimization of the ISSR-PCR reaction system of Quercus glandulifera var.brevipetiolata[J];Journal of Fujian Forestry Science and Technology;2006-01
9 BI Quan-xin1,JIN Ze-xin1,ZENG Zhi-cheng1,YE Wen-guo2,LI Jian-hui1 (1.Ecological Research Institute of Taizhou College,Linhai Zhejiang 317000,China;2.Tiantai County Forestry Bureau,Tiantai,Zhajiang 317200,China);The optimization of ISSR-PCR conditions of Liquidambar formosana[J];Journal of Fujian Forestry Science and Technology;2008-01
10 SONG Wen-jing1,2,JIN Ze-xin2,LI Jun-min2,LI Jian-hui2(1.College of Life and Environmental Science,Hangzhou Normal University,Hangzhou 310036,Zhejiang,China;2.Institute of Ecology,Taizhou College,Linhai 317000,Zhejiang,China);Optimization of ISSR-PCR System of Fagus hayatae[J];Journal of Fujian Forestry Science and Technology;2009-02
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 Ren Xiufang (Department of Biology);The Studies of Stem and Leaf Anatomy of a endemic Species of China—monimopetalum Chinese Rend[J];Journal of Anhui Normal University(Natural Science);1989-04
2 HAO Chao-yun; GUO Wei-Dong; LIU Peng. Department of Biological Science, Zhejiang Normal University, Jinhua, 321004, P. R. China.;Primary study on genetic divergence within populations of Heptacodium miconioides.[J];Journal of Beijing Forestry University;2005-02
3 Peng Hai, Zhang Jing, Zhang Liang, Chen Chanyou;Screening for the Optimum Annealing Temperatures of ISSR-PCR Primers of Asparagus Bean[J];Journal of Changjiang Vegetables;2007-09
4 HE Qiao, LIANG Guo-lu, XIE Jiang-hui (College of Horticulture and Landscape , Southwest Agricultural University, Beibei, Chongqing 400716 China;Southern Subtropical Crop Research Institute , Chinese Academy of Tropical Agricultural Sciences ,Zhanjiang, Guangdong 524091 China);Optimization of the inter-simple sequence repeat reaction system and its preliminary application in wax apple(Syzygium samarangens)[J];Journal of Fruit Science;2005-02
5 DAI Hong-yan,ZHANG Zhi-hong,ZHOU Chuan-sheng,LI He,GUO Xiu-wu (College of Horticulture,Shenyang Agricultural University,Shenyang,Liaoning 110161 China);Establishment and optimization of ISSR system in Crataegus spp.[J];Journal of Fruit Science;2007-03
6 LI Wen-biao~ 1,2 , ZHOU Xian-ye~1, LI Yong~2, ZHANG Shou-zhou~ 2* ( 1.College of Life Sciences, South China Normal University, Guangzhou 510631, China; 2.Shenzhen Fairy Lake Botanical Garden, Shenzhen 518004, China );Discussion on the ISSR reaction condition of Trachycarpus fortunei[J];Guihaia;2006-02
7 FAN Yan-ping,LI Hui-ling,LI Hao-jian(College of Horticulture,South China Agric.Univ.,Guangzhou 510642,China);Pigment Composition and Ultrastructural Difference of Chloroplast in Three Kinds of Variegation Leaf of Cymbidium sinense[J];Journal of South China Agricultural University;2006-02
8 HE Ye-hua1,LIU He-ping1,LIU Song-song2,YI Qing3,YE Yong-chang2,LIN Shun-quan1,L Chang-ping3 (1 College of Horticulture,South China Agricultural University, Guangzhou 510642,China;2 Forestry Institute of Dongguan,Dongguan 523006,China;3 College of Horticulture,Hunan Agricultural University,Changsha 410128,China);Variation and Regulation on Chimera Variety of Dracaena Fragrans During in vitro Culture[J];Journal of South China Agricultural University;2008-02
9 YI Qing1,HE Ye-hua2,LIU Song-song3,Lü Chang-ping1,LIU He-ping2,LIN Shun-quan2,ZHU Jian-yun3,YE Yong-chang3(1.College of Horticulture and Landscape,HNAU,Changsha 410128,China;2.South China Agriculture Guangzhou 510642,China;3.Forestry Institute of Dongguan,Dongguan,Guangdong 523006,China);Establishment of in vitro propagation system with high-efficiency based on three cultivars of Dracaena fragrans[J];Journal of Hunan Agricultural University(Natural Sciences);2007-04
10 WANG Jie-ming XIANG Yan WU Da-qiang SUN Zhi-juan CAI Cheng(College of Forest and Garden,Anhui Agricultural University,Hefei,Anhui 230036);ESTABLISHMENT OF AN ISSR REACTION SYSTEM FOR POPLAR AND OPTIMIZATION USING ORTHOGONAL DESIGN[J];Journal of Nuclear Agricultural Sciences;2007-05
【Secondary References】
Chinese Journal Full-text Database 4 Hits
1 TANG Yan-qiong1,HU Xin-wen1,GUO Jian-chun2,BAI Chang-jun3,HE Hua-xuan3(1.College of Agriculture,Hainan University,Danzhou 571737,China;2.Institute of Biotechnology and Bioscience,CATAS,Haikou 571101,China;3.Institute of Tropical Crops Genetic Resources,CATAS,Danzhou 571737,China);An analysis by ISSR of genetic diversities in Stylosanthes germplasms[J];Acta Prataculturae Sinica;2009-01
2 LI Hui-hui,ZHU Yu-qiu,SI Jin-pin,GAO Yan-hui(Lab of Biotechnology,Zhejiang Forestry College,Lin'an 311300,China);Establishment of ISSR Reaction System of Trichosanthes kirilowii Maxim[J];Biotechnology;2009-06
3 Huang Chunqiong1,2,3,Liu Guodao1,2 1 Tropical Crop Genetic Resources Institute,CATAS,Danzhou,Hainan 571737;2 Ministry of Agriculture Key Laboratory for Utilization of Tropical Crop Germplasm Resources,Danzhou,Hainan 571737,China 3 College of Agronomy,Hainan University,Danzhou,Hainan 571737;Optimization of ISSR-PCR in Cynodon dactylon[J];Chinese Journal of Tropical Crops;2009-11
4 KONG Yan,ZHANG Hao-xing,XIE Xiao-junSchool of Life Science,Southwest University,Chongqing 400715,China;Optimization of the ISSR-PCR System on Coreius heterodon(Bleeker) by Orthogonal Design[J];Journal of Southwest University(Natural Science Edition);2010-02
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 XI Jia-bin~1, CHEN Ping~2, ZHENG Yu-zhong~1, YANG Zhong-yi~(1*)(1. School of Life Sciences, Sun Yatsen University, Guangzhou 510275, China; 2. Agricultural Department, Zhongkai Agrotechnical College, Guangzhou 510225, China);An investigation of Axonopus compressus germplasm resources in China[J];Acta Pratacultural Science;2004-01
2 WANG Yan-hua~(1,2),HOU Xi-lin~(1,*),XU Ming-yu~1gricultural University,Nanjing 210095,China;2 College of Horticulture, Hebei Agricultural University, Baoding 071001,China);Study on optimization for ISSR reaction system in Brassica campestris ssp.chinensis using orthogonal design[J];Acta Botanica Boreali-occidentalia Sinica;2004-05
3 Zheng Jingsheng 1,2 Lü Bei 1 1 Hainan Provincial Key Laboratory of Crop Molecular Breeding,Sanya,572025 2 The Institute of Rice&wheat,Fujian Academy of Agricaltural Sciences,Fuzhou,350019;PCR Technique and its Practical Methods[J];Molecular Plant Breeding;2003-03
4 Xuan Jiping,Zhang Zhen ,Fang Jinggui ,Gao Zhihong,and Che Shengli (College of Horticulture,Nanjing Agricutural University,Nanjing,Jiangsu210095China);Construction of Apple Ccultivar IS SR Fingerprinting[J];Journal of Fruit Science;2002-06
5 QiaoYushan, Zhang Zhen, Fang Jinggui, ShenZhijun, and Zhao Mizhen (College of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu 210095 China; Research Institute of Horticulture, JiangsuA-cademy of Agricultural Sciences, Nanjing, Jiangsu 210014 China);Establishment of the Inter-simple Sequence Repeats Reaction System in Plum Germplasm[J];Journal of Fruit Science;2003-04
6 Zhang Qinglin and Luo Zhengrong  (College of Horticulture and Forestry,Huazhong Agricultural University,Wu han,Hubei430070China);ISSR Technology and Its Applications in Fruit Trees[J];Journal of Fruit Science;2004-01
7 Qiu Yingxiong Fu Chengxin(College of Life Sciences, Zhejiang University\ Hangzhou310029) He Yunfang(Management Station of Seed and Seedling,Zhejiang Forestry Bureau\ Hangzhou310029);IDENTIFICATION OF MICHELIA TSOI TYPES USING ISSR-PCR MARKER ASSAYS[J];Scientia Silvae Sinicae;2002-06
8 Yin TongmingHan ZhengminHuang MinrenWang MingxiuLi Shuxian (Forest Genetics and Gene Engineering Laboratory Nanjing Forestry UniversityNanjing210037);RAPD Experiment Protocol and Optimization in Forest Trees[J];JOURNAL OF NANJING FORESTRY UNVERSITY;1999-04
9 MA Cui-lan 1, LIU Xing-hui 1, ZHANG Yu-lan 2, (1. Department of Horticulture,Fujian Agricultural University,Fuzhou,350002; 2. College of Agronomy,Inner Mongolia Agricultural University,Huhhot,010018);APPLICATIONS OF DNA MOLECULAR MARKERS TO FRUIT TREES[J];Journal of Inner Mongola Institute of Agriculture and Animal Husbandry;2001-02
10 He Yuqing; Zhang Yu; Sun Mei; He Guangchun; Wang Songwen; Zhu Yingguo ( College of Life Science, Wuhan University, Wuhan 430072; The Department of Zoology, Hong Kong University; The Department of Agronomy, Tianin Agricultural College, Tianjin 3003ll );Studies on the Relationship between Cultivated and Wild Rice Using ISSR Markers[J];Journal of Agricultural Biotechnology;2001-02
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved