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《Guihaia》 2011-01
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Effects of He-Ne laser on photosynthesis of wheat seedlings exposed to enhanced UV-B radiation

GAO Li-Mei,LI Yong-Feng,HAN Rong(College of Life Science,Shanxi Normal University,Linfen 041004,China)  
The wheat seedlings(jinmai 8)were exposed to He-Ne laser(5 mW·mm-2),enhanced UV-B radiation(10.08kJ·m-2·d-1)and the combined He-Ne laser irradiation and enhanced UV-B radiation.After being treated for different days(4,5,6,7,8 d)circularly,the changes of the rate of electron transport and relative permeability of chloroplasts membrane,fluorescence emission spectrum were tested with electric conductor and low temperature fluorescence spectrometry.The activities of phosphoenolpyruvate carboxylase(PEPC),carbonic anhydrase(CA),glycolate oxidase(GO),malate dehydrogenase(MDH),chlorophyllase(chlase)and ATPase were measured by photometer.The results showed that the rate of electron transport,the activities of PEPC,CA and ATPase had declined by enhanced UV-B radiation.However,the relative permeability of chloroplasts membrane,the activities of GO,MDH and chlase had increased by enhanced UV-B radiation.Distribution of excitation energy also changed.So the photosynthetic activities of chloroplasts were almost lost.However,the certain dose of He-Ne laser irradiation could partly repaire these damages induced by enhanced UV-B radiation.
【Fund】: 国家自然科学基金(37671061);; 山西省自然科学基金(2008011059-1)~~
【CateGory Index】: S512.1
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