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《Guihaia》 2018-02
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Location of berberine and development of apoplastic barriers on Coptis chinensis anatomcal structure

HU Lujie;WANG Xiao'e;YANG Xiaolin;YANG Chaodong;ZHANG Xia;Engineering Research Center of Ecology and Agriculture Use of Wetland,Ministry of Education,Yangtze University;  
Goldthread( Coptis chinensis) is a famous medicinal plant in Ranunculaceae. We studied berberine locate in tissue and organ features,and apoplastic barriers developed in adventitious roots and rhizomes,and anatomic structures of adventitious roots,rhizmoes and petioles in different development stages. All samples were collected from Lichuan farmland,Hubei Province. We carried out the cell wall histochemistry under light and epifluorescence microscope,and the berberine in organ and tissue fluoresced yellow under blue exciting light that was refered as berberine hemisulfate-aniline stain for lignin which combine with lignin and fluoresce stronger yellow,berberine hemisulfate-aniline stain for Casparian bands fluoresced vivid yellow,and lignin fluoresced stronger yellow. Sudan red 7 B stain for suberin showed reddish. PhloroglucinolHCl stain for lignin showed cherry reddish. Primary structure of goldthread adventitious roots compose of vascular cylinder,endodermis,cortex,exodermis and epidermis; secondary structure compose of secondary xylem,secondary phloem and phellem. Primary structure of rhizomes compose of cuticle,cortex and vascular cylinder; secondary structure compose of phellem,cortex and vascular cylinder. Petioles compose of pith,sclerenchyma rings with vascular bundles,cortex and cuticle. The apoplastic barriers in adventitious roots compose of endodermis and exodermis; and endodermis,exodermis and phellem present in same development stages; and phellem in secondary structure. The apoplastic barriers in rhizmoes compose of cuticle in young stage,and phellem in age stage. Berberine deposite on primary xylem and secondary xylem of adventitious roots and rhizmoes,and on sclerenchyma rings of petioles. Xylem and sclerenchyma rings are important for medicinal quality of goldthread. Exodermis develop early and berberine anchor in primary xylem to root tips,which may prevent water and mineral to absorbing and transporting in goldthread adventitious root. The endodermis and exodermis and phellem of apoplastic barriers totally restrict water and mineral that transport in adventitious roots,among which only very few surface of root tips can absorb water and mineral; and berberine deposit and block up the xylem that result in slowing down of water and mineral transport,which explains why goldthread growth is very slow and need 5-6 years to harvest. These structure characteristics maybe the reasons for goldthread adapting to shade environment.
【Fund】: 湿地生态与农业利用教育部工程研究中心开放基金(KF201603)~~
【CateGory Index】: S567.52
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