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《GUIHAIA》 1998-02
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A study on tissue culture and rapid propagation ofCymbidium sinense and its hybrids in vitro

Zeng SongjunCheng Shijun Zhang JingliZhao Fengban Huang Xiangli(South China Botanical Garden, Chinese Academy of Science, Guangzhou 510520)  
In this paper, the tissue culture and rapid propagation of embryo, shoot, flower bud, leaf and root of Cymbidium sinense and its hybrids were studied. the results are as follows: The suitable mediums are 1/2 MS + NAA 0.5 mg/L + 10% coconut juice in embryo sprouting and MS + 6-BA 0.5 mg/L + NAA 0.5 mg/L + 0.5% active carbon in culture of shoot and flower bud. The culture of leaf and root had not succeeded. G3 that mainly includes Plyponex No. 1, Plyponex No. 2, Bacto-peptone and active carbon is the best medium in propagating of khizomei. The optimun medium of inducing buds form khizomei is B5 + 6-BA 2~3 mg/L + NAA 0.2 mg/L + 0.5% active carbon. Growth of seedling and induction of root are the fastest in 1/2 MS+NAA 2.0 mg/L+10% banana juice + 10% apple juice. The optimun sucrose concentration of medium is 3% except the medium in inducing root, which is 2%. Hybrids have heterosis to its mother in khizomei propagation, bud and root induction, seedling growth, disease resistance and so on.
【Fund】: 中国科学院华南植物园科学基金
【CateGory Index】: S682.310.3
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