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《GUIHAIA》 1999-02
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The study on isozyme and soluble proteins of intraspecies differentiation in Allium hookeri

Wan Haiqing Liang Mingshan Xu Jiemei (Department of Biology, Sichuan Union University, Chengdu 610064)  
In this paper, isozymes of POX, EST of bionts of 3 populations and POX, EST, G6PDH, ME MDH, AAT, ATPase and LDH of 16 populations of A hookeri and soluble proteins of 3 of the 16 populations were analysed by means of polyacrylamide gel electrophoresis The results indicate that the isozymes are non-different among the bionts of one population, the isozymes patterns and soluble proteins ones are remarkably different among the populations with each showing characteristic isozymes and soluble proteins pattern onthe basis of the Rf value and isozymes activity Moreover, the resemblance of the isozymes patterns of the 16 populations of A hookeri were analysed using the methods of arranging and clustering analysis The results indicate that perennial A hookeri, which include all three triploids populations, Pingshan, Ruyuan and Longsheng populations, and such diploids as Dafeishui,Emei Qingyinge, Muchuan, Qingshen, Wulong and Nanchuan populations studied in our research, are closely related among various populations within the same type, whereas non-perennial A hookeri populatins, which wither during the winter, are also closely related to each other within the same type Differentiation level of perennial populations is rather high The originality of this paper exists in the application of IEF/SDS-PAGE in the research of intraspecies differentiation of Allium, and our experiments prove that this research approach is more indicative and persuasive In conclusion, isozymes and soluble proteins can be genetic markers of the genetic differences and relationship among intraspecies And they provide reliable biochemical basis for the study of intraspecies differentiation of plant
【Fund】: 国家自然科学基金
【CateGory Index】: S633.301
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