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《China Medicine and Pharmacy》 2012-11
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The application of trace cell culture method in the EV71 virus research

FAN Rongjun LI Xiaopeng LIANG Shuang LIANG Ying HUAI Qingjie REN Lina Harbin Center for Disease Control and Prevention,Harbin 150056,China  
Objective To construct a trace cell culture method for isolating the hand-foot-and-mouth virus and screening drugs to inhibit the EV71 virus.Methods The differences of EV71 virus detection using a combination of trace cell culture and real-time PCR or single real-time PCR were studied,and inhibition effects of ribavirin against EV71 were investigated through the trace cell culture using cytopathic effect and MTT analysis.Results Through the trace cell culture,EV71 and CA-16 positive detection ratio values were 90% and 80%,respectively;and the EV71-positive value was 20% in PE negative samples.With the ribavirin concentration of 0.4,0.2,0.1 mg/mL,the inhibition ratio values against EV71 virus were 30.28%,28.09% and 29.61%,respectively.Conclusion The trace cell culture method is feasible for the isolating and detecting the hand-foot-and-mouth virus,and also the screening of inhibition drugs.Meanwhile,this method is more simple,convenient and time-saving.
【Fund】: 黑龙江省哈尔滨市科技创新人才研究专项资金项目(2009RFQQS007)
【CateGory Index】: R725.1
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