Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《China Medicine and Pharmacy》 2012-11
Add to Favorite Get Latest Update

The application of trace cell culture method in the EV71 virus research

FAN Rongjun LI Xiaopeng LIANG Shuang LIANG Ying HUAI Qingjie REN Lina Harbin Center for Disease Control and Prevention,Harbin 150056,China  
Objective To construct a trace cell culture method for isolating the hand-foot-and-mouth virus and screening drugs to inhibit the EV71 virus.Methods The differences of EV71 virus detection using a combination of trace cell culture and real-time PCR or single real-time PCR were studied,and inhibition effects of ribavirin against EV71 were investigated through the trace cell culture using cytopathic effect and MTT analysis.Results Through the trace cell culture,EV71 and CA-16 positive detection ratio values were 90% and 80%,respectively;and the EV71-positive value was 20% in PE negative samples.With the ribavirin concentration of 0.4,0.2,0.1 mg/mL,the inhibition ratio values against EV71 virus were 30.28%,28.09% and 29.61%,respectively.Conclusion The trace cell culture method is feasible for the isolating and detecting the hand-foot-and-mouth virus,and also the screening of inhibition drugs.Meanwhile,this method is more simple,convenient and time-saving.
【Fund】: 黑龙江省哈尔滨市科技创新人才研究专项资金项目(2009RFQQS007)
【CateGory Index】: R725.1
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 7 Hits
1 YIN Shengping JIANG Yin QIN Ping QIU Minghao LI Qiaoyu GE Shanfei Department of Infectious Diseases,the Fourth Affiliated Hospital of Guangxi Medical University,Liuzhou 545005,China;Treatment of fifty-two sever hand foot and mouth disease summing-up[J];China Medicine and Pharmacy;2012-03
2 Liu Zhao, Yang Zhanqiu, Xiao Hong, et al Institute of Virology, School of Medicine, Wuhan University, Wuhan 430071,China;A Simple and Rapid Method of MTT Assay for Evaluation of Antiviral Activity[J];Journal of Hubei Medical University;2004-03
3 ZHANG Zheng-jun,XIAO MinDepartment of Emengency,Taihe hospital Affiliated to Yunyang Medical college,Shiyan,hubei 442200,China;Effect of Tanreqing injection on anti-respiratory syncytial virus(RSV,long strain) in vitro[J];Journal of Medical Forum;2011-05
4 DI Biao, et al (Sanitation and Anti-Epidemic Station of Guangzhou City,510080);ISOLATION OF INFLUENZA VIRUSE BY USING MICROPLATE METHOD[J];Disease Surveillance;2001-01
5 TIAN Yan,LU Zhi-gang,LIANG Jie.(Shihezi Center for Disease Control and Prevention,Xinjiang 832000,China);RESEARCH DEVELOPMENT OF CHECK METHODS FOR HAND,FOOT AND MOUTH DISEASE[J];Modern Preventive Medicine;2011-15
6 LIANG Ying,HUAI Qing-jie,ZHANG Bing-li,LIANG Shuang,REN Li-na,FAN Rong-jun,LI Xiao-peng (Harbin Center for Disease Control and Prevention,Harbin 150056,China);Investigations on influence of two methods on detection of EV71 virus[J];Chinese Journal of Health Laboratory Technology;2010-07
7 XU Chun-hua(Electrical and electronic engineering technology department,Dalian Vocational and Technical College,Liaoning Dalian,116037,China);The Effect of Soy Isoflavone on Mouse Erythroleukemia Cell Line and Rat Liver Cancer Cell Line By MTT[J];Journal of Minzu University of China(Natural Sciences Edition);2012-01
Chinese Journal Full-text Database 10 Hits
1 GAO Yu-gang et al (Traditional Chinese Medicine College,Jilin Agricultural University,Changchun,Jilin 130118);Research on the Anti-virus of Antlers Polysaccharide[J];Journal of Anhui Agricultural Sciences;2010-22
2 CAI Bing-yan et al(Jiangsu Animal Husbandry & Veterinary College,Taizhou,Jiangsu 225300);Effects of Compound Acid Preparation against Newcastle Disease Virus in vitro[J];Journal of Anhui Agricultural Sciences;2010-26
3 LIN Hong1a,HU Ge1a,SUO Zhan-wei1a,YI Peng-fei2,HU Yi-yi3,MU Xiang1b(1.a.Department of Animal Science and Technology;b.Beijing Key Laboratory of VeterinaryMedicine Graditional Chinese Medicine and Pharmacology,Beijing University of Agriculture,Beijing,102206,China;2.College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062,China;3.Institute of Traditional Chinese Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China);Expression of IFN-γ of Pulmonary Microvascular Endothelial Cells Induced by Chinese Herbal Medicinal Constituents[J];Journal of Beijing University of Agriculture;2008-03
4 WANG Ai-ping1,ZHAN Song-he2,ZHU Liang-qiang1,QI Ke-zong1,YAO Xue-jing1(1.College of Animal Science and Technology,Anhui Agricultural University,Hefei,Anhui,230036,China;2.Anhui Animal Epidemic Disease Prevention and Control Center,Hefei,Anhui,230036,China);Inhibitive Effects of APS,RBV and IFN on PRRSV in vitro[J];Progress in Veterinary Medicine;2010-03
5 WEI Lan-ping,CHEN Jian-xin,HE Zi-hua,LU Chun-ni,LI Jin-feng,HUANG Xian-hui,FANG Bing-hu(College of Veterinary Medicine,South China Agricultural University,Guangzhou,Guangdong,510642,China);Effect of Hypericin on Influenza Virus H9N2 in Vitro[J];Progress in Veterinary Medicine;2010-12
6 CHANG Yang;ZHOU Youcai;DUAN Shumin;GONG Junxiang;Heilongjiang Aolida Ned Pharmaceutical Co., Ltd.;Heilongjiang Aolida Medical Group;Virus Diseases Prevention and Control Institute, China Center for Disease Prevention and Control;Heilongjiang Aolida Pharmaceutical Co., Ltd.;;Experimental study of inhibition and prevention effect of Niuhuang Qinggan capsules for H3N2 influenza virus A[J];China Medicine and Pharmacy;2013-16
7 LU Gui-ping,CAI Bing-yan,LI Ju-yin(Jiangsu Animal Husbandry & Veterinary College,Taizhou 225300,Jiangsu,China);Anti-NDV Activity of Achyranthes bidentata Polysaccharide Sulfate In Vitro[J];Hubei Agricultural Sciences;2011-04
8 Liu Yuanyuan, Yang Zhanqiu, He Jing, et al Laboratory of Clinical Virology, Institute of Virology, School of Medicine, Wuhan University, Wuhan 430071, China;Comparison and Analysis of CPE Assay, MTT Assay and Plaque Reductive Assay for Evaluating Medicines in Antiviral Effects in vitro[J];Journal of Hubei Medical University;2005-02
9 LUO Fan~1,HOU Wei~1,YANG Zhanqiu~1,HAO Jianxin~2,TATSUO Suzutani~3,SHUICHI Nori~3~1State Key Laboratory of Virology & Institute of Virology,School of Medcine,Wuhan University,Wuhan 430071,China~2Dept.of Pharmacology,Hubei College of Traditional Chinese Medicine,Wuhan 430061,China~3Dept.of Microbiology,Fukushima Medical University,1 HIKarigaoka,Fukushima 960-1295,Japan;Antiviral Activity of Polygonum Paleaceum Wall Against Influenza Virus in Vitro[J];Medical Journal of Wuhan University;2006-01
10 WU Chun-yan1,2,HE Le1,HUANG Hui-qin1,ZHU Jun1,SUN Qian-guang1,BAO Shi-xiang1*(1.Institute of Tropical Bioscience and Biotechnology,CATAS,Haikou 571101,China; 2.College of Environment and Plant Protection,Hainan University,Haikou 570228,China);Screening of anti-H1N1 actinomycetes from marine sponge and identification of strain HA10201[J];Chinese Journal of Marine Drugs;2012-01
China Proceedings of conference Full-text Database 2 Hits
1 ;深圳流感监测网络病毒分离标本采集质量评估[A];[C];2008
2 GAO Yu-gang,LIU Jia-jia,YANG He,ZHANG Lian-xue,ZANG Pu (College of Chinese Traditional Medicine,Jilin Agricultural University,Changchun,Jilin,130118, China);The Screening of Chinese crude drug of Anti-bovine viral diarrhea virus from sika[A];[C];2009
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Lu Yingnian, Wang Qiubo, Wu Hong, et al Department of Immunology, Qingdao Medical College, Qingdao 266021;A FEASIBLE STUDY OF TETRAZOLIUM BASED COLORIMETRIC ASSAY FOR DRUG SCREENING[J];;1997-03
2 CUI Ai-li~ 1, XU Wen-bo~ 1, LI Xiu-zhu~ 2, HU Jia-yu~ 2, LING Hua~ 3, TANG Wei~ 2, YANG Zhi-hong~ 4, ZHANG Yan~ 1, CHEN Li~ 1, Hiroyuki Shimizu~ 5(1. National Institute for Viral Disease Control and Prevention, China CDC, Beijing 100052, China;2. Shanghai Center for Disease Control and Prevention, Shanghai 200336, China;3. Chongqing Center for Disease Control and Prevention, Chongqing 400042, China;4. Childrens Hospital, Fudan University, Shanghai 200032, China;5. National Institute of Hygiene, Japan);Identification of Enterovirus Type 71 by RT-PCR and the Gene Characterization[J];Chinese Journal of Virology;2004-02
3 Pan Zhaozhao1,Wang Xuefeng2,Yue Zhijun2,Yan Lijuan2,Nan Chunhong2(1.Liaoning University of TCM,110032,Shenyang,China;2.The Hospital Affiliated to Liaoning University of TCM,110032,Shenyang,China);Empirical studies on the extracts from compatibility of Flos Lonicerae and Forsythia suspense inhibiting influenza A virus FM1 strain in vitro[J];Journal of Gansu College of Traditional Chinese Medicine;2007-02
4 Shen Yuan ying, Yang Zhan qiu, Liu Jian Jun,et al Institute of Virology, Medical College, Wuhan University, Wuhan 430071, China;Pathological Observation of Infection of Coxsakievirus B_3[J];Journal of Hubei Medical University;2001-04
5 LI Yong mei 1, LI Li 2, BAI Chuan 1, LI Ding 1, WANG Tian zhi 1 1. West China School of Pharmacy,Sichuan University,Chengdu 610041,China; 2. Department of Biology, Fudan University,Shanghai 200437,China;EFFECT OF EXTRACTS FROM HONEYSUCKLE FLOWER ON ANTI-ADENOVIRUS[J];West China Journal of Pharmaceutcal Sciences;2001-05
6 TIAN Wen, SONG Shao bai, LU Hao ying, ZHENG Wei, TAN Hua, TANG Yun, WANG Yan, YU Yan xing (Department of General Surgery, PLA General Hospital, Beijing 100853);Studies of the effect of a fusion protein Fab/IFN αA on hepatitis B virus in vitro[J];ACADEMIC JOURNAL OF PLA POSTGRADUATE MEDICAL SCHOOL;2000-02
7 CHEN Ze-bin,ZHOU Shao-ming.Children′s Hospital of Shenzhen City,Guangdong,Shenzhen 518026,China;Clinical observation of efficacy of ribavirin sparay on hand-foot-and-mouth disease[J];Chinese Journal of Clinical Rational Drug Use;2010-08
8 JIA Yan.The Children′s Special Hospital of Chengdu,Chengdu,Sichuan 610015,China;The effect observation of Reduning injection combined with ribavirin in children with hand-foot-mouth disease[J];Chinese Journal of Clinical Rational Drug Use;2011-15
9 LIU Pei-hui,YANG Yue-ping,ZOU Xin-ying.(Shenzhen Municipal Maternal Hospital affiliated to Nanfang Medical University,Shenzhen 518028,Guangdong,P.R.China);Observation on the effect of Ganciclovir on 29 infants with hand-foot-mouth disease[J];China Tropical Medicine;2007-07
10 XU Mei ling,YU Jian er,PAN Xin,et al(28);Clinical and Experimental Study of “Pneumonia Formula” in Treating Coxsackie Virus Pneumonia[J];SHANGHAI JOURNAL OF TRADITIONAL CHINESE MEDICINE;2000-08
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved