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《Industrial Microbiology》 2001-02
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Effects of carbon and nitrogen sources on the culture of recombinant Anabaena sp. PCC7120

LIU Zhi-wei 1, GUO Yong 1, ZHANG Chen 2 (1.College of Food Engineering and Biotechnology, South China University of Technology, Guangzhou, 510640; 2.Department of Biology, Jia Ying Unversity, Meizhou, Guangdong, 514015)  
The effects of carbon and nitrogen sources on the culture of Anabaena sp. PCC7120 harboring pDC-TNF were studied. The results showed that sucrose and NaNO 3 were the optimum carbon and nitrogen sources. When 9 g/L of sucrose and 2.25 g/L of NaNO 3 were used, the biomass was 2.52 g/L which was 71.66% greater than culturing in BG-11 under the same condition, and the expression and bioactivity of TNF were 16%~22% and 10 5 U/mg, respectively.
【Fund】: 广东省自然科学基金资助项目 ( 980 5 41)
【CateGory Index】: Q943.1
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