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《Industrial Microbiology》 2002-02
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Effects of environment factors on the culture of recombinant Anabaena sp. PCC7120

ZHANG Chen 1, LIU Zhi-wei 1, GUO Yong 2 (1.Department of Biology, Jia Ying College, Meizhou, Guangdong 514015; 2. College of Food Engineering and Biotechnology, South China University of Technology, Guangzhou 510640)  
The mixotrophic culture of recombinant Anabaena sp. PCC7120 harboring pDC-TNF was studied in shake flask and the optimum culture conditions were as follows. volume of inoculum was 5%, strength of illumination was 1000lux, temperature was 25~30℃, volume of media in 100ml flask was 40mL,and pH kept at 8.3 during the culture of recombinant Anabaena sp. PCC7120 in BG-11 media containing 9g/L sucrose and 2.25 g/L NaNO 3, the harvest biomass was over 3 g/L, the content of dissoluble protein and the expression of TNF was about 30% and more than 22%, respectively. The results showed that mixotrophic culture was the way for the recombinant Anabaena sp. PCC7120 to achieve high density and high expression.
【Fund】: 广东省自然科学基金资助项目 ( 980 5 41)
【CateGory Index】: Q943.1
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