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《Industrial Microbiology》 2008-03
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Protoplast formation,regeneration and UV mutagenesis of natamycin producing Streptomyces gilvosporeus

LI He-di,JIN Zhi-hua,ZHANG Hai-guang,JIN hua (Department of Biological & Pharmaceutical Engineering,Ningbo Institute of Technology,Zhejiang University,Ningbo 315100)  
The conditions of protoplast formation,regeneration and UV mutagenesis of Streptomyces gilvosporeus were studied.It was beneficial under the conditions of late exponential phase cell(48 h) with 0.4% lysozyme at 30℃ for 90min. After regeneration,about 73.3% strains' production of natamycin was increased,and the production of natamycin by mutant 5-12 reached 2121.2μg/mL,which was 74.7% more than that of the parent strain.After UV mutagenesis,five high producing strains were obtained,and the production by UV-2 was 107.09% more than that of the parent strain.Its production reached 2515.07μg/mL.
【Fund】: 浙江省自然科学基金项目(No.Y404291)
【CateGory Index】: TQ465.5
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【Citations】
Chinese Journal Full-text Database 1 Hits
1 CHENG Ji1, HONG Wen-rong1, SU Jian-zhang1, YE Li-rong2 (1. College of Qiaoxing Light Industry, Fuzhou University, Fuzhou, Fujian 350002, China; 2. Fuzhou Antibiotic General Factory, Fuzhou, Fujian 350002, China);Study on protoplast fusion between Streptomyces tenebrarius and Streptomyces kanamyceticus[J];Journal of Fuzhou University(Natural Sciences Edtion);2003-01
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 NIU Li-na et al (Key Laboratory of Bio-resources and Eco-environment,Ministry of Education,Sichuan University,Chengdu,Sichuan 610064);Study on the Streptomycin Resistance Screening of High-yield Strain of Jinggangmycin[J];Journal of Anhui Agricultural Sciences;2007-28
2 PENG Yi et al(Agro-environmental Protection Institute,Ministry of Agriculture,Tianjin 300191);Study on the streptomycin resistance screening of Streptomyces hygroscopicus strains highly producing agricultural antibiotics[J];Journal of Anhui Agricultural Sciences;2008-18
3 WEI Dong~1,TAN Hui-lin~2,YANG Hai-yan~2,CUI Chun-sheng~1,YING Li-xia~2(1.Institue of Microbiology,Xinjiang Academy of Agriculture Sciences,Urumqi 830091;2.The Food Science of Xinjiang Agriculture University,Urumqi 830091,China);Breeding of L-Serine-Producing Strain and Optimization of Fermentation Conditions[J];Amino Acids & Biotic Resources;2006-01
4 DING Lin(Teaching Center of Experimental Biology,Collage of Life Science,Wuhan University,Wuhan 430072,China);Compound Mutagenesis and Screening of High Productivity Strains of Lincomycin[J];Amino Acids & Biotic Resources;2006-04
5 CUI Pei-wu,LI Ji-lie,WEN Rong,HUANG Ling,YANG Jie(Fermentation Engineering Lab,College Life of Science and Technology,Central South University of Forest and Technology,Changsha,Hunan 410004,China);Breeding of High-yield Naringinase Strain from Penicillium sp. P-M1 by UV Mutation[J];Northern Horticulture;2009-02
6 WEI Jue1,GUO Yurong2,LUO Wen1,FU Zhongxu1(1.College of biotechnology industry,Chengdu University,Chengdu 610106,China;2.College of Food Engineering and Nutritional Science,Shanxi Normal University,Xi'an 710062,China);Combination Mutagenesis of Specific α-linolenic Acid Lipase Producing Strains[J];Journal of Chengdu University(Natural Science Edition);2008-04
7 YANG Yong-bin,HUANG Yan-yan,LIN Yue-xin(Bioengineering College, Fujian Normal University, Fuzhou 350007, China);Strain Breeding of Chrysemonas luteola Producing Alkaline Cellulose by Mutagen[J];Journal of Fujian Normal University(Natural Science);2005-01
8 LI Yang,LIU Feng*,LU Long-di(College of Life Sciences,Fujian Normal University,Fuzhou 350108,China);Screening of Mutant Overproducing L-tryptophan with Sulfonamide Resistance Method and Optimization of the Fermentation Conditions[J];Journal of Fujian Normal University(Natural Science Edition);2009-03
9 YANG Kai,GUO Dai-ping,LI Zhong-qin,XU Xiao-ping(College of Chemistry and Chemical Engineering,Fuzhou University,Fuzhou,Fujian 350002,China);Asymmetric biosynthesis of R-adrenalin from corticosterone[J];Journal of Fuzhou University(Natural Science Edition);2007-06
10 ZHANG Xiao-yong~(1,2),LIN Bi-run~1,GAO Xiang-yang~2,SHEN Hui-fang~1(1.Plant Protection Research Institute,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,China; 2.College of Life Science,South China Agricultural University,Guangzhou 510642,China);New technology for mutagenizing and screening high-yield strains in agricultural antibiotic[J];Guangdong Agricultural Sciences;2006-05
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 ZHU He-shui et al(College of Animal Husbandry and Veterinary,Henan Agricultural University,Zhengzhou,Henan 450002);Application of Protoplast Fusion Technology[J];Journal of Anhui Agricultural Sciences;2007-05
2 WANG Chun-ping1,2,WEI Qiang1,BAO Guo-lian1,LIU Yan1,SHAO Ze-xiang1,2,JI Quan-an1(1.Animal Husbandry and Veterinary Institute,Zhejiang Academy of Agricultural Sciences,Hangzhou,Zhejiang,310021,China;2.College of Animal Science and Veterinary Medicine,Shandong Agricultural University,Tai'an,Shandong,271018,China);Progress on Microbial Protoplast Fusion Technology[J];Progress in Veterinary Medicine;2008-05
3 Wang Hui, Tong Yingkai, Wu Zhaoliang, Hu Jin'gang;Research on screening of high yielding flavomycin strain by mitrous acid mutation[J];Feed Industry;2006-08
4 Zhi Yu LIU; Rui Fang WANG; Yue Hai SHEN (Shanghai Institute of Organic Chemistry Chinese Academy of Sciences,354 Fenglin Lu. Shanghai 200032);Synthesis of the C_(11-16)+C_(27) Segment of Epothilone A[J];中国化学快报(英文版);1998-01
5 CHEN Jie,QIU Hong-duan,LIN Juan,XIE Hang,ZHAO Jie(College of Biological Science and Technology,Fuzhou University,Fuzhou,Fujian 350002,China);Study on the preparation condition of protoplasts separated from Rsp capsulata and Bacillus stearothermophilus[J];Journal of Fuzhou University(Natural Science Edition);2007-02
6 CHEN Wei-wei,ZHU Hong-yang,XU Hong (College Of Life Science and Pharmaceutical Engineering,Nanjing University of Technology,Nanjing 210009);Breeding of mass-producing ε-polylysine mutant and its batch fermentation[J];Industrial Microbiology;2007-02
7 Tan Wen-hui,Li Yan-ping,Xu Yang (The Key Laboratory of Food Science of Ministry of Education (Nanchang University); Sino-Germany Joint Research Institute,Nanchang 330047,China);Factors Affect the Formation and Regeneration of Protoplasts of Microorganism[J];Modern Food Science and Technology;2006-03
8 LUO Li-xin, LU Yi-ming, PAN Li (College of Biological Science and Engineering, South China University of Technology, Guangzhou 510640, China);Screening of High Epothilone-producing Mutants of Sorangium cellulosum by NTG Mutation[J];Modern Food Science and Technology;2007-03
9 QIU Yan-lin, LIANG Liang, WANG Liang (The province key laboratory of Industrial Microorganism, College of Bioengineering, Hubei University of Technology, Wuhan 430068, China);Mutation Screening of L-histidine Producing Strain by UV and LiCl[J];Modern Food Science and Technology;2008-03
10 HU Ting-ting,QIU Yan-lin(Institute of Bioengineering,Hubei University of Technology,Wuhan 430068,China);Screening and Breeding of Strain Producing High Yield Cellulase through Irradiation of Protoplast by UV[J];Chemistry & Bioengineering;2008-08
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