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《INDUSTRIAL MICROBIOLOGY》 1998-03
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RP HPLC Rapid Analysis of Organic Acids during High Cell Density Culture of Recombinant E.coli YK537/pSB TK

Yang Ruyan,Li Min,Chen Changqing (Shanghai Research Center of Biotechnology,Chinese Academy of Sciences,Shanghai 200233)  
Various organic acids produced by bacteria during high cell density culture of recombinant E.coli YK537/pSB TK were rapidly analysed by HPLC with reversed phase colmn C 18 .Ideal separation and quantitative analysis of organic acids were achieved by ether and water extractio,which could remove most of interferential peaks in the sample.Results indicated that acetic acid decreased using glycerol as carbon resource for bacteria growth in place of glucose,while the inhibition of cell growth and heterologous protein expression decreased,so it would be easy to achieve high cell density and high expression culture of recombinant E.coli.
【CateGory Index】: Q939.97,
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【References】
Chinese Journal Full-text Database 4 Hits
1 YANG Ru yan, LI Min, CHEN Chang qing (Shanghai Research Center of Biotechnology,Chinese Academy of Sciences,Shanghai 200233);Determination and optimization of glycerol during the high density fermentation of genetic engineered bacteria[J];INDUSTRIAL MICROBIOLOGY;1999-01
2 SONG Hui~1,WANG Shu-ying~1 ,ZOU Wei-hua~2,et al(1.Department of Veterinary Medicine ,Shandong Agriculutral Univeristy ,Taian 271018,China;2. Department of Life Science,Shandong Agriculutral Univeristy ,Taian 271018,China);Relationship between the high density fermentation of avian Escherichia coli and dissolved oxygen[J];Heilongjiang Journal of Animal Science and Veterinary Medicine;2004-01
3 Li Min, Chen Changqing (Shanghai Research Center of Biotechnology,Chinese Academy of Sciences,Shanghai 200233);Progress Studies of High Cell-density Culture of Recombinant Escherichia coli[J];PROGRESS IN BIOTECHNOLOGY;2000-02
4 LI Min 1, XU Hao 1, ZHOU Yi-Ming 2, YANG Ru-Yan 1, CHEN Chang-Qing 1 (1. Shanghai Research Center of Biotechnology, Chinese Academy of Sciences, Shanghai 200233; 2. Shanghai No.1 Biochemical & Pharmaceutical Co, Shanghai 200040);Study on High Cell-Density Fermentation of Recombinant E.coli Expressing rhTNFα[J];CHINESE JOURNAL OF PHARMACEUTICALS;2000-10
【Citations】
Chinese Journal Full-text Database 3 Hits
1 Xu Hao, Li Min, Ruan Changgeng*, Cheng Changqing(Shanghai Research Center of Biotechnology, Chinese Academy of Scienses, Shanghai 200233)*(Department of Hematology, Suzhou Medical College, Suzhou 215007);Production of Mutant Recombinant Human TumorNecroses Factor by High Density Cell Culture[J];INDUSTRIAL MICROBIOLOGY;1998-02
2 GAO Chang-Shou; MAO Shen-Lan;YU Ying and OHEN Chang-Qing (Shanghai Research Centre of Biotechnology, Academia,Sinica 200233);Overexpression of a Novel Highly Active Tumor Necrosis Factor Mutant in E. coli[J];ACTA BIOCHIMICA ET BIOPHYSICA SINICA;1996-01
3 Ding Mingyu, Chen Peirong and Luo Guoan (Department of Chemistry, Tsinghua University, Beijing, 100084);High Performance Liquid Chromatographic Analysis of Organic Acids in Foods[J];CHINESE JOURNAL OF CHROMATOGRAPHY;1997-03
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 WU An-quan,YANG Kai-lun~*,LUO Qiu-jiang,NIU Chao-feng,HU Jian-yong,ZHU Wen-yuan(Laboratory of Animal Nutrition,Xinjiang Agricultural University,Urumqi 830052,China);Effects of N-Acetyl-DL-methionine Administered Ruminally on the Rumen Digestion and Metabolism in Sheep[J];Grass-Feeding Livestock;2006-02
2 Niu Chao-feng,Yang Kai-lun,Hu Jian-yong,et al(Laboratory of Animal Nutrition,Xinjiang Agricultural University,Urumqi 830052,China);Effect of Supplemental Lysine and Schiff's bases of Lysine on the Ruminal Fermentation of Sheep[J];China Herbivores;2007-05
3 CHEN Fa-xing~(1,2), LIU Xing-hui~2, LIN Hua-ying~3, CHEN Li-song~2 (1.Institute of Subtropical Fruit, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China;2.College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China;3.Fuzhou Disease Prevention and Control Center, Fuzhou, Fujian 350004, China);Determination of the organic acids from the fruit and leaf of loquat by ion-exchange chromatography[J];;2004-02
4 GUO Gen-he, PAN Wei, SU De-sen, LAO Qiu-hua, HE Zhi-gang, CHEN Han-zhen ( Central Laboratory, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China; Institute of Pomology, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, China);Determination of some organic acids in the fruits of loquat by RP -HPLC[J];Fujian Journal of Agricultural Sciences;2005-03
5 ZHANG Ze-huang~(1),SHI Huai~(2),XU Jia-hui~(1),Lin Qi-hua~(1)(1.Pomology Institute,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350013,China;2.Biotechnology Institute,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350003,China);Organic acid metabolism of wampee(Clausena lansium) fruits during ripening and low temperature storage[J];Fujian Journal of Agricultural Sciences;2006-04
6 NUER Gu-li,RAN Zhu-ye,ZHAO Xiao-feng,ZHU Jing,YU Juan,MA Yue (Petrochemical Institute,Karamay Petrochemical Subcompany,Petrochina,Karamay 834000,China);HPLC Determination of the Organic Acid Content in Fermentation Liquor of In-situ-biological Process for Oil Production[J];Analysis and Testing Technology and Instruments;2007-02
7 Wei Wei,Hu Ping,Wang Yiming,Wang Ruji,Luo Guoan* (Department of Chemistry,Tsinghua University, Beijing l00084);Investigation on Separation Behavior in Capillary Ion Exchange Electrochromatography[J];Chinese Journal of Analytieal Chemistry;1997-10
8 Liu Chenming~(1,2),Cao Hongbin~(*1),Cao Junya~(1,2),Li Yuping~(1,2),Zhang Yi~(1) ~1(Institute of Process Engineering,Chinese Academy of Sciences,Beijing 100080) ~2(Graduate University of Chinese Academy of Sciences,Beijing 100049);Fast Detection of Organic Acid in Anaerobe Medium with Gradient Elution-High Performance Liquid Chromatography[J];Chinese Journal of Analytical Chemistry;2006-09
9 Mou Ying~ 1 , Wang Yuanhao~ 1 , Xiu Zhilong~ *1 , Wang Longxing~ 2 , Xiao Hongbin~ 2 ~ 1 (Department of Bioscience and Biotechnology, School of Environmental and Biological Science and Technology, Dalian University of Technology, Dalian 116024) ~ 2 (Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023);Determination of Organic Acids in 1,3-Propanediol Fermentation Broth by Reversed Phase High Performance Liquid Chromatography[J];Chinese Journal of Analytical Chemistry;2006-S1
10 ZHANGZhongyi (A Postgraduate of the Material and Chemical Industry Department of Zhejiang University, The Professional Technique College of Lishui T College, 323000);Application introduction of Supercritical Fluid in Extraction[J];Journal of Chemical Education;2003-06
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 Fu Huaxin et al (Wujin Plant Protection and Quarantine Station, Wujin 213017);Studies on the Technique of Applying Harpin for the Diseases control of strawberry Grown in Greenhouse[J];Journal of Anhui Agricultural Sciences;1999-06
2 Yi Jiping;Liu XuZhang;Yu Dunshou(Reaserch Center of Bioengineering Wuhan University 430072);Study on the Replacement of Rice Bran with Malt Sprou in Lactic Acid Fermentation[J];AMINO ACIDS & BIOTIC RESOURCES;1996-02
3 Zhang Zhiqing Yao Lihong Hou Yunde(National Laboratory of Molecular Virology and Genetic Engineering, Institute of Virology, Chinese Academy of Preventive Medicine, Beijing );CONSTRUCTION AND APPLICATION OF A HIGH LEVEL EXPRESSION VECTOR CONTAINING P_RP_L PROMOTER[J];Chinese Journal of Virology;1990-02
4 Liu Yunhuan 1,Chen Donghai 2 (1.2HenanUniversity Of Technology,Zhengzhou 450052 );Study Progress on Microbial Degradation of Organophosphates[J];North Environment;2005-01
5 YUAN Qi peng SUN Chang hui (College of Chemical Engineering, Beijing University of Chemical Technology, Beijing 100029, China);Effects of oscillation operations on plasmid stability of S. cerevisiae harboring plasmid[J];JOURNAL OF BEIJING UNIVERSITY OF CHEMICAL TECHNOLOGY;2002-02
6 YANG Qing-li,YANG Xiu-quan (China Research Institute of Daily Chemical Industry,Taiyuan 030001,China);Effect of surfactant on stability of protease used for liquid detergents[J];China Surfactant Detergent & Cosmetics;2005-05
7 Sun Fengyu, Wu Ming, Li Wenzhao, Li Xinyong, Gu Wanzhen, Wang Fudong(Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian 116023);RELATIONSHIP BETWEEN CRYSTALLITE SIZE AND PHOTOCATALYTICACTIVITY OF TITANIUM DIOXIDE[J];CHINESE JOURNAL OF CATALYSIS;1998-03
8 DOU Hong 1, LI Min 2, MAO Ji Fang 1, CHEN Chang Qing 2 (1. Department of Biochemistry and Molecular Biology, Department of Basic Medicine, Second Military Medical University, Shanghai 200433, China; 2. Shanghai Research Centre of Biotechnology, Acad;High cell-density culture of E. coli TG1/pGEX-hCT and high expression of recombinant human calcitonin[J];ACADEMIC JOURNAL OF SECOND MILITARY MEDICAL UNIVERSITY;2000-03
9 YE Bing, WANG Yun ji, ZHANG Ling hua (School of Bio. & Food Eng., Dalian Inst. of Light Ind., Dalian 116034,China);High density fermentation of recombinant producing phytase Pichia pastoris[J];Journal of Dalian Institute of Light Inoustry;2002-03
10 LI Yu ping, LI Rong fen, ZHOU Jian xin (Department of Biochemistry and Molecular Biology, Third Military Medical University, Chongqing 400038, China);Cloning and expression of cDNA encoding human membrane-bound stem cell factor(SCF)[J];Acta Academiae Medicine Militaris Tertiae;2001-10
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 YIN Shu-qin et al(College of Life Sciences,Shanxi Agricultural University,Taigu,Shanxi 030801);Research on High Cell Density Culture of Genetic Engineering Bacteria[J];Journal of Anhui Agricultural Sciences;2007-11
2 WU Ya-li~1,LIU Dong~2,ZHANG Li-jun~2,LI Shi-min~2,LIANG Shi-zhong~1(1.School of Bioscience and Biotechnology,South China University of Technology,Guangzhou 510640;2.School of Chemical and Biological Engineering,Shenzhen Polytechnic,Shenzhen 518055,China);Optimized Culture of Engineered Bacteria(E.coli/pGEX) by Orthogonal Test[J];Amino Acids & Biotic Resources;2006-01
3 WANG Hai Bo1, WANG Li Li2, OU Jun Jie2, GENG Xin Du21Department of Chemistry, School of Basic Medicine, Fourth Military Medical University, Xian 710033, China, 2Institute of Modern Separation Science, Key Laboratory of Modern Separation Science in Shaanxi Province, Northwest University, Xian 710069, China;A method of high density fermentation of Escherichia coli in producing rhG-CSF[J];Journal of The Fourth Military Medical University;2004-12
4 GUO Yan1, HUI Chang-ye2 1National Hepatobiliary and Enteric Surgery Research Center of Ministry of Health, Central South University, Changsha, 410078, China;2Department of Microbiology, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515, China;Pilot-scale production and quality control of multiepitope hepatitis B virus DNA vaccine[J];Journal of Southern Medical University;2009-01
5 ZHANG Xi-fen1,2,YANG Zeng-qi1(1.College of Animal Science and Technology,Northwest A & F University,Yangling,Shaanxi,712100,China;2.Yangling Lvfang Bio-engineering Co.LTD,Yangling,Shaanxi,712100,China);Study on Conditions of High Density Fermentation in Piglet Escherichia coli[J];Progress in Veterinary Medicine;2007-09
6 ZHANG Qiu-xuan,LI Min*,CHEN Deng-long,HUANG Xi,LU Bao-yong,ZHOU Zhi-hua (College of Life Sciences,Fujian Normal University,Fuzhou 350108,China);Optimization of the Conditions of High Cell Density Fermentation by Orthogonal Test for the Engineering Bacterial Strain with RGD-spider Silk Protein Gene[J];Journal of Fujian Normal University(Natural Science Edition);2007-05
7 KONG Hong-Xing LI Li-Jun WEI Hong-Guang YAN Liu-Juan (Department of Light and Chemical Engineering,Guangxi University of Technology,Liuzhou,Guangxi 545006,P.R.China);Kinetic Analysis on the Oxidation of Glycerin with Sodium Periodate and Its Application[J];Chinese Journal of Spectroscopy Laboratory;2003-01
8 WANG Jian-feng 1, XIU Zhi-long 2, FAN Sheng-di 1 (1. Department of Chemical Engineering, Dalian Nationality College, Dalian 116600 ; 2. Department of Biotechnology, Dalian University of Science, Dalian 116012);Determination of glycerin concentration during the fermentation of glycerin to 1,3-propanediol[J];Industrial Microbiology;2001-02
9 TU Gui-yun, LI Min (Biology Institute of Fujian Normal University, Fuzhou 350007);Research progress of high density fermentation process of genetic engineering microorganisms[J];Industrial Microbiology;2004-03
10 ZHANG Xi~(1),FAN Dai-di~(2),HUA Xiu-fu~(2),LUO Yan-e~(2),MA Xiao-xuan~(2) (1.College of Life Science,Northwest University,Xi'an 710069,China;2.College of Chemical Engineering,Northwest University,Xi'an 710069,China);Study on Nitrogen-feeding of Recombinant Escherichia coli for Producing Human-like Collagen[J];Chemistry & Bioengineering;2006-01
China Proceedings of conference Full-text Database 1 Hits
1 Xue Xiao-Chang,Bao Chun-Jie,Han Wei,Qin Xin,Zhang Cun,Cao Zhu-Rong,Li Wei-Na,Zhao Yu-Hong,Zhang Yingqi Biotechnology Center of Fourth Military Medical University,Xian 710032;High Cell Density Fermentation of Recombinant Human B Lymphocyte Stimulator[A];[C];2008
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Li Jinchang , Shi Jing (Centre of Analysis and Test,Northeast Mormal University,Changchun 130024);Determination of Fruit Acid by High-performance Liquid Chromatography[J];Chinese Journal of Analytical Chemistry;1993-08
2 Gao Zhifeng, Fu Chengguang (Centre of Physical and Chemical Analysis, Hebei University, Baoding 071002);Determination of Organic Acids by Nonsuppressed Ion Exclusion Chromatography With Conductometric Detection[J];Chinese Journal of Analytieal Chemistry;1994-12
3 GAO Chang-Shou; MAO Shen-Lan;YU Ying and OHEN Chang-Qing (Shanghai Research Centre of Biotechnology, Academia,Sinica 200233);Overexpression of a Novel Highly Active Tumor Necrosis Factor Mutant in E. coli[J];ACTA BIOCHIMICA ET BIOPHYSICA SINICA;1996-01
4 YangGeng sheng,Zh u Yan and Zhuang X iangping, Testing & Technology Institute of Zhejiang Province,Hangzzhou;Simultaneous Determination of Ascorbic Acid, Sac- charine, Benzole Acid and Sorbic Acid in Beverages by High Performance Liquid Chromatography[J];Chinese Journal of Chromatography;1989-05
5 Zhu Yan andTang Cengsheng, Zhejiang Provincial Institute of Test Techniques, Hangzhou, 310012.;Determination of Organic and Inorganic Acids in Beverages by Ion Chromatography[J];Chinese Journal of Chromatography;1990-01
6 Wang Yujun,Li Lieying,Instiute of Oceanology,Academia Sinica,Qingdao,266071; Sun Yongle and Yang Wenfu,Occupational Safely and Health Research Institute Ministry of Chemical Industry,Qingdao,266071;Study on the Determination of Organic Acids in Rice Wine by High Performance Liquid Chromatogrophy[J];Chinese Journal of Chromatography;1991-04
7 Wu Fengwu and Zhang Ping,Wuhan Municipal Anti-Epidemic Station,430014;Simultaneous Determination of Nine Food Additives in Beverage by High Perfonnance Liquid Chromatography (HPLC)[J];Chinese Journal of Chromatography;1992-05
8 Yan Jinying. Shi Yinyu. Dep. of Chem. . Zhejiang University, Hangzhou, 310027;The Advances in the Analysis of Low Molecular Carboxylic Acids by High Performance Ion Exclusion Chromatogra phy (HPIEC )[J];Chinese Journal of Chromatography;1993-01
9 Yu Hong . Liu Qinglin and Guan Liangzi, Department of Chemistry, Harbin Normal University , 150080;Simultaneous Analysis of Inorganic Anions and Organic Acids by Single-Column Ion Chromatography[J];Chinese Journal of Chromatography;1993-02
10 Ding Haibiao and Zhang Shenghua, Food Science and Techmology Department,Huazhong Agricultural University, Wuhun ,430070;Measuring L -Ascorbic Acid in Orange Juice by Reversed-Phase High Performance Liquid Chroma- tography (HPLC)[J];Chinese Journal of Chromatography;1993-03
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