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RP HPLC Rapid Analysis of Organic Acids during High Cell Density Culture of Recombinant E.coli YK537/pSB TK

Yang Ruyan,Li Min,Chen Changqing (Shanghai Research Center of Biotechnology,Chinese Academy of Sciences,Shanghai 200233)  
Various organic acids produced by bacteria during high cell density culture of recombinant E.coli YK537/pSB TK were rapidly analysed by HPLC with reversed phase colmn C 18 .Ideal separation and quantitative analysis of organic acids were achieved by ether and water extractio,which could remove most of interferential peaks in the sample.Results indicated that acetic acid decreased using glycerol as carbon resource for bacteria growth in place of glucose,while the inhibition of cell growth and heterologous protein expression decreased,so it would be easy to achieve high cell density and high expression culture of recombinant E.coli.
【CateGory Index】: Q939.97,
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