Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Industrial Health and Occupational Diseases》 2012-01
Add to Favorite Get Latest Update

Effects of nickel sulfate on ultramicrostructure alterations of rat leydig cells in vitro

ZHENG Jing,ZHANG Zhi-gang,SUN Ying-biao, SU Li,XIA Hu,LI Cheng-yun,ZHANG Rui,LI Jin(Institute of Health Toxicology,School of Public Health,Lanzhou University,Lanzhou 730000 China)  
Objective By observing the ultramicrostructure alterations of leydig cells to investigate the injury features of leydig cells induced by nickel sulfate(NiSO4)in vitro.Methods Leydig cells were isolated using collagenase dispersion.The density gradient centrifugation with Percoll separating medium and differential adhesion assay were used to purify leydig cells,then the leydig cells were identified with 3β-HSD staining;Leydig cells of exponential growth phase were treated with different concentrations of nickel sulfate at doses of 0.0,62.5,125.0,250.0,500.0 and 1000.0 μmol/L,and the proliferation of leydig cells was detected with MTT chromatography after separately exposed for 3 h,6 h and 12 h.Transmission electron microscopy(TEM)was used to observe the ultramicrostructure changes of leydig cells after exposed for 6 h.Results The cytoplasm of leydig cells were stained specifically in blue black with 3β-HSD staining,and the purity of leydig cells was above 95%;Compared with the control group,125.0 μmol /L and above concentrations could significantly inhibit the growth of leydig cells(P0.05 or P0.01);The various abnormal changes of mitochodria and endoplasmic reticulum were observed with TEM including mitochondrial swelling,ectasia and degranulation of smooth endoplasmic reticulum and increased lysomal in NiSO4 500.0 μmol/L and 1000.0 μmol/L group especially.Conclusions NiSO4 can induce the ultramicrostructure alterations of rat leydig cells.
【Fund】: 中央高校基本科研业务费专项资金(Lzujbky-2009-89)
【CateGory Index】: R114
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 2 Hits
1 LI Jian;HU Xiao-ting;ZHANG Cai-xia;WANG Liang;TAO Xi;SU Li;ZHAO Chi;SUN Ying-biao;Institute of Health Toxicology,school of Public Health,Lanzhou University;;Effect of nickel sulfate on steroidogenic enzymes of rat Leydig cells in vitro[J];Industrial Health and Occupational Diseases;2014-03
2 BIN Bin;LU Haiwang;WANG Jie;WANG Desheng;XU Jiexin;QIN Zhibiao;CUI Jinzhu;LIANG Jingsong;PANG Qiuhua;The First Affiliated Hospital of Guangxi University of Chinese Medicine;;Overview on Studies of Relevant Factors Affecting Ultrastructure of Testicular Epididymis and Sperm[J];Liaoning Journal of Traditional Chinese Medicine;2013-11
【Citations】
Chinese Journal Full-text Database 2 Hits
1 WU Cai-hong1,2,ZHANG-Bin1,ZHENG Xiao-feng1,RUI Rong3,DAI Jian-jun41.Jiangsu Animal Husbandry and Veterinary College,Taizhou 225300;2.Jiangsu JiangQuHai Pig Breeding Farm,Taizhou 225300;3.The College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095;4.Institute of Animal Husbandry & Veterinary Science,Shanghai Academy of Agricultural Sciences,Shanghai 201106;Manufacturing of SEM and TEM Samples for Oocyte[J];农业科学与技术(英文版);2009-05
2 Sun Xiangzhou, Deng Chunhua, Guo Haibin, Liu Jianzhong Department of Urology, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China;Experimental study of allograft Leydig cells transplantation of animal model of PADAM[J];Chinese Journal of Andrology;2005-03
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 LI Peng et al(Pharmacy College,Xinxiang Medical University,Xinxiang,Henan 453003);Inhibitory Effect of Agrimol on K562 Leukemia Cell[J];Journal of Anhui Agricultural Sciences;2011-22
2 CAO Jin PAN Qi-chao(Department of Anticancer Drug Research; Cancer Institute) ( Sun Yan-Sen University of Medical Sciences, Guangzhou 510060);THE DAMAGES OF THE TOTAL ALKALOID OF HARMALA ON THE ULTRASTRUCTURE OF RETICULUM-CELL SARCOMA L2 CELLS IN MICE[J];Chinese Journal of Cancer;1993-03
3 WANG Ming-shu1,2,CHENG An-chun1,2,LIU Wu-mei1,3,ZHOU Yi1,2,GUO Yu-fei1,2,YUAN Gui-ping1,2,CHEN Xiao-yue1,2(1.College of Animal Science & Veterinary Medicine,Sichuan Agricultural University,Yaan 625014,Sichuan,China;2.Key Laboratory of Animal Disease and Human Health of Sichuan Province,Yaan 625014,Sichuan,China;3.College of Aquiculture Science of Sichuan Agricultural University,Pixian 611700,Sichuan,China);Studies on Porcine Reproductive and Respiratory Syndrome Virus Morphogenesis in Marc-145 Cell and the Ultrastructural Changes of the Infected Cells[J];Chinese Journal of Virology;2007-01
4 LU¨ Jiu Quan ① LU Ji Qi ① NIU Hong Xing ① WANG Yu Rong ② (① Department of Biology, Henan Normal University ② Bio production company, Henan Normal University Xinxiang\ 453002,China);Anatomical Observations of the Digestive Tract of the Pachyhynobius shangchengensis[J];CHINESE JOURNAL OF ZOOLOGY;2000-02
5 WANG Yu(Department of Biology,Longnan Teachers College,Chengxian Gansu 742500,China);An Observation of Histological Structure of the Digestive Tract in Japalura Splendida[J];Journal of Beijing Union University(Natural Sciences);2011-03
6 Yao Po;Li Shi Feng Department of Biology, Guangzhou Teachers' College Guangzhou Fishery Rescarch Institute;MICROSCOPIC AND SUBMICROSCOPIC INVESTIGATION OF THE SHRIMP MACROBRACHIUM ROSENBERGII BRAIN GANGLION AFTER OVIPOSITION[J];Fisheries Science;1993-06
7 Xia Zhongfu(Pohl Institute,Tongji University,Shanghai 200092);Biomaterials of Electret[J];Materials Review;1994-01
8 Qu Liandong and Chu Guijun(Harbin Veterinary Institute of China Agricultural Academy)Teng Guolin,Zhang Weimu and Yang Guijun (Northeast Agricultural University, Harbin 150030,China);STUDIES ON THE DYNAMIC ULTRAMICROPATHOMORPHLOGY OF LIVER AND KIDNEY OF EXPERIMENTAL CHRONIC FLUOROSIS IN DOMESTIC RABBITS[J];JOURNAL OF NORTHEAST AGRICULTURAL UNIVERSITY;1994-04
9 Jing Songyan, Zhang Wei, Wang Linggang, Hu Xiaoying(Northeast Forestry University, Harbin 150040, P R China); Yu Yueming(Heilongjiang Native Produce Company);Impact of Molting Dose Injection of Cyclophosphamide on the Skin Tissue and Molting in Raccoon Dogs[J];JOURNAL OF NORTHEAST FORESTRY UNIVERSITY;1998-02
10 CHEN Quan-zhen,HU Xi-gang,GAO Ai-gen,YANG Jun-yi,WANG Xiao-gu (Second Institute of Oceanography,SOA,Hangzhou 310012,China);Ultrastructure of major organ in abalone Haliotis discus hannai[J];Donghai Marine Science;2002-02
China Proceedings of conference Full-text Database 2 Hits
1 SUN Jing-qiu~1 XU Yan~1 ZHANG Hui-qi~1 YE Jun-qiang~2 (1.College of Life and Environment Sciences,Shanghai Normal University,Shanghai 200234,China; 2.Shanghai Fishery Technical Extension Center,Shanghai 201400,China);Study on Cytochemical Location of Acid Phosphatase and Alkaline Phosphatase of Litopenaeus vannamei[A];[C];2008
2 SUN Ying-biao~1 CHEN Jian-hua~2 ZHAO Jian-xiong~3 ZHU Yu-zhen~1 (1.School of Public Health,Lanzhou University,Lanzhou Gansu 730000,China);Effect of Chinese herbs of FuZhengJieDu granules on the nickel sulfate-induced damage of testis germ cells in rats[A];[C];2007
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 Wei Youhuan,Huang Qiuchan,Su Xiufang(Department of Chemistry and Biology Engineering,Nanning Teachers College,Longzhou 532400,China);Review on the Toxicological Effect and the Mechanism of Nickel to the Human Health[J];Environmental Science and Management;2008-09
2 ZHANG Ming-jun,GUO Xian-kun,ZENG Fan-hui,et al.(Reserch Institute of Andrology,Medical College,Three Gorges University,Yichang 443003,China);Electron Microscopic Technique to Assess Effect of rhTNF-a on Ultra Structure of Human[J];Journal of Hubei Institute for Nationalities(Medical Edition);2008-01
3 LU Wei-li,DENG Zhi-nian,WEI Yuan-wen (Guangxi Cropgenetic Improvement and Biotechnology Lab,Nanning 530007,China);Nickel and Biology[J];Trace Elements Science;2003-10
4 LI Qingren,SU Bin,LI Shengchuan(College of Chemistry,Jilin Normal University,Jilin Siping 136000,China);Trace Element Cobalt,Nickel and Human Health[J];Guangdong Trace Elements Science;2008-01
5 ChenQiongyu et al(Dept.of Lab. Hyg. & Occup. Diseases, Henan Med. Univ.,40, Daxue Rd., Zhengzhou 450052 China);TOXICITY STUDY OF NICKEL CHLORIDE ON ISOLATED RAT LIVER[J];INDUSTRIAL HEALTH AND OCCUPATIONAL OISEASES;1995-06
6 Ma Mingyue et al (Dept. of Toxicity, Shenyang Med. College, 78, S-Huanghe St., Shenyang 110031 China);PROTECTIVE EFFECT OF SODIUM SELENITE ON NICKEL SULFATE-INDUCED EMBRYOTOXICITY IN RATS[J];INDUSTRIAL HEALTH AND OCCUPATIONAL OISEASES;1996-05
7 Li Chong-bin,Sun Fa,Xiao Yue-hai,Shi Jia-qi,Gu Jiang,Liu Xian-lin,Chang Ao-shuang,Xie Chun.Department of Urology,the Affiliated Hospital of Guiyang Medical College,Guiyang,550004,Guizhou,China.Department of Electron Microscopy,Guiyang Medical College,Guiyang,550004,Guizhou,China.College of Public Health,Guiyang Medical College,Guiyang,550004,Guizhou,China.;Effects of coal-burning fluorosis on the ultrastructure of rat testis[J];Guizhou Medical Journal;2011-02
8 Shan Guang,Zhang Xiaobing,Yang Cixing(Department of Urology,The People's Hospital of Wuhan University,Hubei 430060,China);PROTECTIVE EFFECT OF ASCORBIC ACID AND THIAMINE SUPPLEMENTATION AGAINST DAMAGE CAUSED BY LEAD IN TESTES OF MICE[J];Chinese Journal of Histochemistry and Cytochemistry;2010-04
9 ZHANC Lin NIU Jing-ping XU Jia,et al.Occupational Health and Environmental Sanitation Institute,School of Public Health,Lanzhou University,Lanzhou,Gansu 730000,China;Comparative Study of Toxic Effects of PM2.5 Collected from Nickel-contaminated Area on Alveolar Macrophages in Rats[J];Journal of Environment and Health;2008-10
10 ZOU Cong,LIA O Xiao-gang,LI Qing-chun,et al.Laboratory of Electron Microscopy,Chongqing Medical University,Chongqing 400016,China;Effect of Maternal Exposure to Trace Cadmium on Ultrastructure and P450scc Expression in Fetal Testicles[J];Journal of Environment and Health;2008-11
【Secondary References】
Chinese Journal Full-text Database 2 Hits
1 BIN Bin;LU Haiwang;WANG Jie;XU Jiexin;WANG Quansheng;WANG Desheng;TIAN Yuanchun;First Affiliated Hospital of Guangxi University of Chinese Medicine;;Study on Effects of Shenqi Qiangjing Capsule on Sperm Acrosin Activity[J];Journal of Liaoning University of Traditional Chinese Medicine;2015-06
2 TANG Qian-li;HE Qing-hu;DAI Bo;LIU Zhao-sheng;ZHOU Qing;HUANG Xin;WANG Quan-sheng;BIN Bin;Key Lab for Western Guangxi High-Incidence Diseases,Youjiang Medical University for Nationalities;School of Integrated Chinese and Western Medicine,Hunan University of Chinese Medicine;Department of Andrology,The First Affiliated Hospital of Guangxi University of Chinese Medicine;Department of Andrology,The First Affiliated Hospital of Hunan University of Chinese Medicine;;Antioxidating and energy metabolism improving effects of Qiangjing Decoction on oligospermia and asthenospermia: An experimental study[J];National Journal of Andrology;2016-02
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 TANG Mei Yi\ ZHANG Chong Li (The State Key Laboratory of Reproductive Biology, Institute of Zoology,;REGULATORY ROLE OF CORTICOTROPIN RELEASING HORMONE ONREPRODUCTIVE FUNCTION[J];ACTA ZOOLOGICA SINICA;1998-04
2 SUN Zhen\|Xing\+①\ WANG Ru\|Cai\+②\ JIANG Ming\+②\ LIU Xiao\|Yun\+② (①Yantai Normal University\ Yantai\ 264025; ②Ocean University of Qingdao Qingdao 266003,China);Effect of Cytochalasin B on the Ultrastructure of Fertilized Eggs of Abalone (Haliotis discus hannai)[J];Chinese Journal of Zoology;2002-05
3 Huang Xiaolan,Yang Mingliang,Wu Xiaomin, et al School of Public Health, Wuhan University, Wuhan 430071, China;Discussion of Protective Mechanism of Lycium Barbarum Polysaccharides on Reproductive System in Rats[J];Journal of Hubei Medical University;2004-01
4 XU Ying-bi1, DING Zhi-en2 1. Environmental Management College of China, Qinhuangdao 066004; 2. Anhui Agricultural University, Hefei 230036;Observation for Leaf Epidermal Characteristics of Four Different Pomegranate Cultivars under Scanning Electron Microscope[J];农业科学与技术(英文版);2008-03
5 LI Li1,21.School of Environmental Studies, China University of Geosciences, Wuhan 430074;2. School of Biological Sciences and Technology, Hubei Institute for Nationalities, Enshi 445000;Obsevation for Epidermal Ultrastructure of Nostoc sphaeroides Kutzing under Scanning Electron Microscope[J];农业科学与技术(英文版);2009-01
6 WANG Xian-zhong WU Jian-yun SUN Yan PAN Hong-mei ZHANG Jia-hua(College of Animal Technology, Southwest Agricultural University, Chongqing,400716);CULTURED LEYDIG CELLS' REACTIVITY TO HUMAN CHORIONIC GONADOTROPN(hCG)[J];Acta Agriculturae Nucleatae Sinica;2005-04
7 Sun Qiang, Chai Jiake, Jiang Enkui, et al. Institute of Burns, First Affiliated Hospital, General Hospital of PLA, Beijing 100037, China;Studies on the effects of TNF-α on rats' Leydig cells and the mechanism of action[J];Medical Journal of Chinese People's Liberation Army;2008-01
8 ZHANG Wei, WU Ting(Department of Urology, the First Affiliated Hospital of NJMU, Nanjing 210029,China);Experimental Study on Allografts of Leydig Cells in Castrated Rats[J];Acta Academiae Medicinae Nanjing;2002-06
9 Ming Han TONG, Hui Bao GAO, Yan Qin HU,Qiang Su GUO (Department of Histology & Embryology,Shanghai Second Medical University,Shanghai 200025);The Study of Glucocorticoid Induced Apoptosis in Cultured Rat Leydig Cells[J];ACTO ANDROLOGICA SINICA;2000-01
10 LIU Jian-zhong~(1),GUO Hai-bin~(2),DENG Chun-hua~(2),OU Yong-hong~(1),PENG Ai-ping~(1)Department of Biology,Preclinical Medicine School,2.Department of Urology,the First Affiliated Hospital,SUN Yat-sen University,Guangzhou,Guangdong 510080,China;The Culture and Identification of Rat Testis Leydig Cell[J];National Journal of Andrology;2006-01
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved