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《Journal of Guiyang Medical College》 2006-06
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The Content Determination of Pineal Melatonin in Old Rats

ZHU Jun-de~1, GONG Ming~2 (1. Department of anatomy, Guiyang Medical College, Guiyang 550004, China;2. Institute of Pharmacology, Guizhou Yibai pharmacy Co Ltd, Guiyang 550002, China)  
Objective: To determine melatonin content in the pineal gland of old rats. Methods: High-performance liquid chromatography (HPLC) was used. Sixteen healthy Sprague-Dawley rats (24-month or older ) were randomly divided into two groups (eight in each, males: females=1∶1). The column used in the analysis was Hypersil ODS2(5.0×200mm), with temperature of 35℃. The detection wave was 225nm, and the mobile phase was methyl alcohol and water (3∶2).The flow rate of the mobile phase was 1.0ml/min. Results: There was linear relationship of melatonin content in pineal gland in the range of 50pg and 1000pg. The regressive equation was y=36.917X+838.36 (r=0.999 8, P0.05), and the lowest concentration for detection was 10pg. Conclusion: The HPLC method is simple, easy to operate, and can be used in basic study of pineal gland.
【CateGory Index】: Q954.6
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