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《Journal of Guiyang Medical College》 2007-03
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The Amplification of ITS Sequence of Gastrodia elata with Polymerase Chain Reaction

XIE Yuan1*,ZHANG Xiaolei2,LI Yi1,ZHANG Ting1,SHAN Ke-Ren1(1.Molecular Biological Key Laboratory of Guiyang Medical College,Guiyang 550004,China; 2.Department of Clinical Laboratory Science,Guiyang Medical College,Guiyang 550004,China  
Objective: To establish a reliable and specific PCR method for the amplification of the internal transcribed spacer(ITS) region of nuclear ribosomal DNA of Gastrodia elata.Methods: The ITS sequence was amplified by PCR with universal primers of ITS.The PCR parameters for ITS sequence amplification were optimized and PCR product was directly sequenced after purification.Results: The PCR product was about 750 bp.There occurred little diversity among the samples from different sources.After sequenced and compared with the optimized sequences in Genbank,the total length of ITS sequence of Gastrodia elata was determined as 598 bp including 235 bp of ITS1,209 bp of ITS2,and 153 bp of 5.8 S,and the(G+C)% was 52%.Conclusions: With the optimized PCR conditions,the amplification of ITS sequence of Gastrodia elata can produce reliable and specific results.
【Fund】: 贵州省科学技术基金项目(黔科合J字[2005]2069号)
【CateGory Index】: R282
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