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《Guangxi Medical Journal》 2011-11
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Correlation Study of Estrogen Receptor and IL-10 in Osteoblasts of Skull in Osteoporosis Rats

QIN Shu-yun1,CAI Ke-jun1,FENG Zhao-shan1,WEI Qi-hou2(1 Liuzhou Medical College,Liuzhou 545006,China;2 Liuzhou Center for Disease Control and Prevention,Liuzhou 545007,China)  
Objective To observe the correlation of estrogen receptor in osteoblasts and IL-10 expression of skull in osteoporosis rats.Methods 40 SD rats were randomly divided into control group(n=20)and ovariectomized group(n=20,5 weeks 10 rats,7 weeks 10 rats).The bone mineral density was measured by ray absorptiometry,IL-10 expression in the skull were detected by immunohistochemical staining of SABC method and the image analysis techniques;The rat osteoblasts were purified by two-step enzymatic digestion method and successive adherence method.The osteoblasts were observed by calcium alkaline phosphatase cobalt method(ALP)and collagen type Ⅰ immunohistochemical staining method.The estrogen receptors of osteoplasts in SD rats were identified by semi-quantitative western blot.Results The ovariectomized group showed less bone mineral density(P0.01);The IL-10 positive osteoblasts in the trabecula of ovariectomized group was less than that of control group(P0.01),and their staining was lighter than control group;the observable results by ALP and collagen type Ⅰ immunohistochemical method corresponded to the morphology of osteoblast features.The estrogen receptor expression of ovariectomized group was significantly lower than that of control group,that of the 7th week was significantly lower than the 5th week in the ovariectomized group(P0.01).Conclusion Estrogen receptor expression is positively correlated with the expression level of IL-10 in the osteoblasts.
【Fund】: 广西壮族自治区教育厅科研基金资助项目(200810MS036)
【CateGory Index】: R580
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