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《Guangzhou Chemical Industry》 2012-16
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Determination of Icariin in Geng Nian San Hao Capsule by HPLC

WU Ding-hong1,QIU Xiao-hui1,ZHANG Zhi-kang2,WANG Xiao-yun1(1 Guangdong Provincial Haspital of TCM,Guangdong Guangzhou 510120; 2 Guangzhou University of Chinese Medicine,Guangdong Guangzhou 510380,China)  
The HPLC method was established for the determination of icariin in Geng Nian San Hao Capsule.The content was determined by HPLC.The analysis was carried on C18 column,the mobile phase was consisted of acetonitrile-water(30:70) with a flow speed at 1.0 mL/min,the UV detection wave length was at 268 nm,and the column temperature was indoortemperature.Sample size was 10 μL.Icariin was in the good linear range when the sample size was between 0.2~8 μg(r=0.9999).The average recovery rate was 100.49%(RSD=1.49%).The method was simple,accurate and can be used to content determination of icariin in the Capsule.
【Fund】: 国家科技“十五”攻关《绝经综合征中医药疗效评价方法研究》(项目编号:2004BA716B02)
【CateGory Index】: R286
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