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《Modern Food Science and Technology》 2010-03
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A Method for Determining β-Glucosidase Activity in Commercial Cellulase

LI Xu-hui1,ZHU Ming-jun1,GUO Qi-jun2(1.School of Bioscience and Bioengineering,South China University of Technology,Guangzhou,510006,China)(2.Jingpo Lake Sugar Indusery Company Ltd of Ningan City,Ningan 157400,China)  
A method for determining β-glucosidase activity in commercial cellulase was established in this paper.β-Glucosidase activity was measured by analyzing glucose in the hydrolysate after hydrolyzed for 30 min at 50 ℃ using cellobiose as substrate.Glucose produced by enzymatic hydrolysis showed good linear correlation with β-glucosidase activity when diluted β-glucosidase activity range in 0.13-0.37 IU/mL.This method was simple,rapid and of high sensitivity.
【CateGory Index】: TS207.3
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