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《Guizhon Medical Journal》 2001-12
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Early apoptosis of mice hepatoma HcaF cells induced by β-elemene

Fang Ling, Cheng Daixong, Wang Haiyang. Cellular engineering Laboratory, Affiliated Hospital of Zunyi Medical College, Zunyi 563003  
Objective To observe the effect of β-elemene on early apoptosis of hepatoma cells(HcaF) in mice. Methods Flow cytometry was used to analyse the early apoptosis of HcaF cells labelled with annexin-V-FITC. Results β-Elemene could induce the early apoptosis of HcaF cells after treatment (50μg/ml) for 2 hours, while it had a significant cytotoxic effect on HcaF cells at high concentration of 100-200μg/ml. Conclusions β-Elemene could induce the early apoptosis of HcaF cells which was associated with concentration of the drug. The annexin-V-FITC labeling is a sensitive and specific marker for evaluating the early apoptosis of cells.
【Fund】: 国家自然科学基金资助课题 (No.39730 440 Ⅱ )
【CateGory Index】: R735.7
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