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《Guangzhou Medical Journal》 2005-02
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Effect of transforming growth factor-α,β and basic fibroblast growth factor on the fibronectin and alkaline phosphatase activity in human periodontal ligament cells in vitro

Ji Jianxin, Liao Weijiao, Qiu Zhihui, et al. Department of Stomatology, the First Affiliated Hospital, Guangzhou Medical College, Guangzhou 510120, China  
Objective To evaluate biological effects in vitro of transforming growth factor-α,β (TGF-α,β) or basic fibroblast growth factor(bFGF) on the fibronection(Fn) and alkaline phosphatase activity(ALPase) in human periodontal ligament(PDL) cells. Methods PDL cells were obtained from healthy donors. Cells(6×10 5/100 μl) were cultured for 4 days in DMEM supplemented with 1% FBS along(control), or containing TGF-α(10 ng/ml), or TGF-β(3ng/ml), or bFGF(3ng/ml).Fibronectin was determined by ELISA. ALPase activity was measured by enzyme kinetic methods. Data was analyzed by ANOVA. Results TGF-β increased fibronectin levels 4.2-fold. TGF-α caused a slight increase in fibronectin levels(134.6%). bFGF did not influence fibronectin levels. TGF-α and bFGF significantly decreased ALPase activity in PDL cells. TGF-β had less effect than the other peptides. Conclusion The data suggest that these growth factors may be valuable in promoting the regeneration of periodontal connective tissue.
【Fund】: 广州市科技局资助项目(编号:1999J00801)
【CateGory Index】: R780.2
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【Citations】
Chinese Journal Full-text Database 5 Hits
1 WT5”BZ] LIU Xiao yong et al.Faculty of Stomatology,Capital University of Medical Science,Beijing 100050 [WT5”HZ];EFFECTS OF BASIC FIBROBLAST GROWTH FACTOR ON THE PROLIFERATION AND ALKALINE PHOSPHATASE EXPRESSION OF HUMAN PERIODONTAL LIGAMENT CELLS.[J];Beijing Journal of Stomatology;2001-02
2 GE Shao hua,YANG Pi shan. (Hospital of Stomatology attached to Shangdong Medical University,Shandong 250012,China)[;Effects of bFGF on Biological Characteristics of Cells Derived from Periodontium[J];Shanghai Journal of Stomatology;2001-01
3 WU Yong,WU Zhi-fen,WANG Qin-tao, et al ( College of Stomatology,The Fourth Military Medical University, Xi'an 710032,China );The combining effects of recombinant human bone morphogenetic protein-2 and basic fibroblast growth factor on the proliferation of human periodontal ligament cells[J];Chinese Journal of Conservative Dentistry;2001-01
4 WU Yong,WU Zhi-fen, WANG Qin-tao ( College of Stomatology,The Fourth Military Medical University, Xi'an 710032, China) $$$$[;The effects of recombinant human bone morphogenetic protein-2 and basic fibroblast growth factor on alkaline phosphatase activity of the periodontal ligament cells[J];Chinese Journal of Conservative Dentistry;2001-04
5 SI Xiaohui, LIU Zheng. Department of Oral Medicine, School of Stomatology, Shanghai Second Medical University, Shanghai 200011, China;The biological effects of transforming growth factor-β on human periodontal ligament fibroblasts[J];Chinese Journal of Stomatology;2001-01
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 GE Shao hua et al Dept.of Stomatology,Shandong University,Jinan,250012;CULTURE,MORPHOLOGY AND PROLIFERATION OF THREE TYPES OF HUMAN PERIODONTAL CELLS[J];Beijing Journal of Stomatology;2003-02
2 ZHOU Dong-qing, LV Ying, LIU Xiao-yong. Capital Medical University School of Stomatology, Beijing 100050, China;Preliminary study of effects of epidermal growth factor on periodontal regeneration of autotransplanted rhesus teeth[J];Beijing Journal of Stomatology;2006-04
3 WANG Yu 1, QIAN Feng Lan 2, WANG Ping 1, YANG Ya Ning 1, HAO Jian Jun 1 1Department of Conservative Densitry, School of Stomatology, Fourth Military Medical University, Xi'an 710033, China, 2Department of Clinical Laboratory, Chengdu Political Fire Brigade Hospital, Chengdu 610042, China;Effects of basic fibroblast growth factor on proliferation and differentiation of human periodontal ligament cells in vitro[J];Journal of The Fourth Military Medical University;2003-23
4 HE Quan-min, LIU Jian-guo, XU Ruo-zhu, ZHANG Jian, FAN Qin, JIANG Ce Affiliated Stomatological Hospital of Zunyi Medical College, Zunyi 563003, China;Effect of tea polyphenols on proliferation of human periodontal ligament fibroblasts[J];Journal of Southern Medical University;2008-08
5 LIU Lan-ning~1,ZHANG Shi-hua~2,WANG Zhen~3.(1.Department of Special Course,the south Campus of Southern University,Guangzhou 510315;2.Zhujiang hospital of Nanfang Medical University,Guangzhou 510282;3.Department of Stomatology,the 94 Hospital of Nanchang,Nanchang 330002,China);The effects of enamel matrix proteins and bone morphogenetic protein 2 on cell proliferation by cultured human periodontal ligament cells[J];Journal of Branch Campus of the First Military Medical;2005-02
6 FENG Ping, LIU Qi, GAO Li, et al. Department of Oral Medicine, Faculty of Stomatology, Zunyi Medical College, Zunyi 563003;The Effects of Transforming Growth Factor-β_1 and Basic Fibroblast Growth Factor on the Proliferation of Human Pe(riodontal) Ligament Fibroblasts[J];Journal of Dental Prevention and Treatment;2005-04
7 YANG Pi-shan+1, SUN Qin-feng+1, SONG Ai-mei+2,GE Shao-hua+1.(1.Dept. of Periodontology, Affiliated Stomatological Hospital;2.Dept. of Dentistry, The Second Hospital, Shandong University, Jinan 250012,China);The Effect of bFGF on Proliferation of Periodontal Fibroblast-like Cells of Dogs[J];West China Journal of Stomatology;2004-01
8 ZHAO Zheng,LIU Hong-chen,E Ling-ling,WANG Dong-sheng.(Institute of Stomatology,Chinese PLA General Hospital,Beijing 100853,China);The effects of different growth factors on biological characteristics of rat dental papilla cells (rDPCs)[J];Chinese Journal of Prosthodontics;2010-03
9 YU Shan-shan,JIANG Xin-quan,WENG Yu-lai,HUA Fei.(Department of Oral Medicine,Ninth People's Hospital,School of Medicine,Shanghai Jiaotong University,Shanghai Key Lab of Stomatology,Shanghai Institute of Stomatology,Shanghai 200011,China);Effects of acidic fibroblast growth factor and transforming growth factor-β1 on proliferation of porcine dental papilla cells[J];Stomatology;2007-07
10 WANG Yan,WANG Shuo-zhi, FAN Ming-wen, et al.School of Stomatology,Wuhan University,Wuhan 430079;A Study of Mineralization in Periodontal Ligament Cell in Vitro-Expression of Bone Sialoprotein and Osteopontin during Mineralization.[J];Journal of Comprehensive Stomatology;2002-01
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 WT5”BZ] LIU Xiao yong et al.Faculty of Stomatology,Capital University of Medical Science,Beijing 100050 [WT5”HZ];EFFECTS OF BASIC FIBROBLAST GROWTH FACTOR ON THE PROLIFERATION AND ALKALINE PHOSPHATASE EXPRESSION OF HUMAN PERIODONTAL LIGAMENT CELLS.[J];Beijing Journal of Stomatology;2001-02
2 Chen Ping;Han Benli;Li Kun;Duan Henchun (Department of Hepatobiliary Surgery, Southwest Hospital, Third Military Medical University,Chongqing, 400038);The effects of activated Kupffer cells on liver cell regeneration in cirrhotic rats[J];ACTA ACADEMIAE MEDICINE MILITARIS TERTIAE;1998-04
3 Zhang Zhiwei, Wu Zaide, Chen Xiaoping (Department of Surgery, Tongji Hospital Tongji Medical University, Wuhan 430030 China);THE INFLUENCE OF LABIATAE ON ENZYMHISTOCHEMI ISCHEMIC LIVER REMNANT IN RATS[J];CHINESE JOURNAL OF HISTOCHEMISTRY AND CYTOCHEMISTRY;1998-03
4 Chen Liming, Zeng Tairan, Tian Maoneng Department of Oral Medicine, Guiyang Stomatological Hospital, Guiyang 550002;Clinical studies of guided tissue regeneration on periodontal disease[J];Guizhon Medical Journal;2002-12
5 Shen Jing 1, Zhu Haiqing 1, Zhao Yi 2, Pan Liquan 2 (1.the Affiliated Brain Diseases Hospital of Nanjing Medical University. Nanjing, Jiangsu, 210029; 2. Nanjing University of TCM. Nanjing, Jiangsu, 210029);Pathologic Observation of TGF-β Reaction of Gastric Wound Tissues Treated by Danshen Yaomo[J];Henan Traditional Chinese Medicine;2004-03
6 Si Xiaohui, Liu Zheng The Ninth People′s Hospital, Shanghai Second Medical University;The Biological Effects of Recombinant Human Bone Morphogenetic Protein 2 on Human Periodontal Ligament Fibroblasts[J];West China Journal of Stomatology;2002-01
7 MAO zhao~ 1, YANG Jian~ 2,MAO Xi~ 3,SU Han~ 1 (1.Department of Odontology , Nanjing General Hospital of PLA , Nanjing 210002 , China;2.Department of pharmacology,Nanjing Medical University, Nanjing 210029 China;3.Bisic Medical college of Dongnan University,Nanjing 210093,China);Effects of chitosan on proliferation and differentiation of human periodontal ligament cells[J];Chinese Journal of Marine Drugs;2005-02
8 Li Jingshan, Ma Jing, Zhu Yaping,et al. (Jining Medical College);INFLUENCE OF RADIX SALVIAE MILTIORRBIZAE ON PHENOTYPICAL EXPRESSION OF FIBROBLASTS[J];JOUNAL OF JINNING MEDICAL COLLEGE;1999-04
9 Ou long, Liu hongwei, Yuan zhiping, Wang dongsheng, Ma liang, Sun wanhua (Department of Stomatology, General Hospital of PLA, Beijing 100853);Evaluation of periodontal guided tissue regeneration (GTR) in an experimental study in dogs[J];ACADEMIC JOURNAL OF PLA POSTGRADUATE MEDICAL SCHOOL;1999-01
10 Li Zubing;A. Norman Cranin; Mark.C Mehrali(College of stomatology, Hubei Medocal University, Wuhan 430070);Experimental study of a bioresorbable guided tissue regeneration menbrane[J];JOURNAL OF COMPREHENSIVE STOMATOLOGY;1998-04
【Secondary Citations】
Chinese Journal Full-text Database 9 Hits
1 ZHAO Yun Fu 1, JIANG Xiao Zhong 1, HU Shi Hui 2, LIU Yuan 1, SONG Chao You 3 (1. Department of Stomatology, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China; 2. Department of stomatology, First People′s Hospital of;Clinical significance of substance P in blood of the patients with trigeminal neuralgia[J];ACADEMIC JOURNAL OF SECOND MILITARY MEDICAL UNIVERSITY;2000-01
2 Zhao Yunfu ,Jiang Xiaozhong,Han Yusheng (Department of Stomatology,Changzheng Hospital,Second Military Medical University,Shanghai,200003);Electron microscopic observation of the affected branches of trigeminal neuralgia[J];ACADEMIC JOURNAL OF SECOND MILITARY MEDICAL UNIVERSITY;1998-04
3 Hu Shihui, Jiang Xiaozhong, Zhao Yunfu, et al. The First Hespital of Yichang, Hubei 443000;Observation of calcitonin gene-related peptide in blood of the patients with trigeminal neuralgia.[J];JOURNAL OF COMPREHENSIVE STOMATOLOGY;1999-04
4 Jiang Xiaozhong, Chen Bishen, Zhao Yunfu, et al. Department of Stomatology, Changzheng Hospital, Second Military Medical University,Shanghai, 200003;Current situation and prospect of surgical treatment for trigeminal neuralgia[J];SHANGHAI MEDICAL JOURNAL;1998-11
5 Si Xiaohui, et al.(Department of Oral Pathology, Stomatological College, the Fourth Military Medical University, Xi’an 710032);Extraction and Purification of TGF β and Experimental Observation of Its Bone Growth Stimulating Effect[J];JOURNAL OF CELLULAR AND MOLECULAR IMMUNOLOGY;1997-01
6 WU Yong,WU Zhi-fen,WANG Qin-tao, et al ( College of Stomatology,The Fourth Military Medical University, Xi'an 710032,China );The combining effects of recombinant human bone morphogenetic protein-2 and basic fibroblast growth factor on the proliferation of human periodontal ligament cells[J];Chinese Journal of Conservative Dentistry;2001-01
7 Wang Qintao, Shi Junnan, Wu Zhifen et al School of Stomatology, The Fourth Military Medical University(710032);The effects of bone morphogenetic protein on the proliferation and alkaline phosphatase activity of periodontal ligament cell[J];CHINESE JOURNAL OF CONSERVATIVE DENTISTRY;1996-03
8 OU Long, LIU Hongwei, PANG Jinfan,et al Department of Stomatology, General Hospital of PLA, Beijing 100853,China;Effects of autogenous periodontal ligament cell transplantation on periodontal tissue regeneration in dogs[J];CHINESE JOURNAL OF STOMATOLOGY;2000-01
9 ZHANG Jin Shan 1, FENG Jin Hua 1, AN Jian Xiong 2, LUO Fei 2, JIN Shu Ming 1, LI Zhong Lian 1 1 Pain Clinic Center, Tianjin First Hospital 300010 2 Sino French Pain Center, Beijing Medical University, Beijing 100083;PLASMA CONTENT OF β ENDORPHIN IN PATIENTS WITH ACUTE ABDOMINAL DISEASE OR CHRONIC SPINAL NEURALGIA[J];CHINESE JOURNAL OF PAIN MEDICINE;1998-01
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