Effect of transforming growth factor-α,β and basic fibroblast growth factor on the fibronectin and alkaline phosphatase activity in human periodontal ligament cells in vitro
Ji Jianxin, Liao Weijiao, Qiu Zhihui, et al. Department of Stomatology, the First Affiliated Hospital, Guangzhou Medical College, Guangzhou 510120, China
Objective To evaluate biological effects in vitro of transforming growth factor-α,β (TGF-α,β) or basic fibroblast growth factor(bFGF) on the fibronection(Fn) and alkaline phosphatase activity(ALPase) in human periodontal ligament(PDL) cells. Methods PDL cells were obtained from healthy donors. Cells(6×10 5/100 μl) were cultured for 4 days in DMEM supplemented with 1% FBS along(control), or containing TGF-α(10 ng/ml), or TGF-β(3ng/ml), or bFGF(3ng/ml).Fibronectin was determined by ELISA. ALPase activity was measured by enzyme kinetic methods. Data was analyzed by ANOVA. Results TGF-β increased fibronectin levels 4.2-fold. TGF-α caused a slight increase in fibronectin levels(134.6%). bFGF did not influence fibronectin levels. TGF-α and bFGF significantly decreased ALPase activity in PDL cells. TGF-β had less effect than the other peptides. Conclusion The data suggest that these growth factors may be valuable in promoting the regeneration of periodontal connective tissue.