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《SUGARCANE》 1998-01
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Purification of Sugarcane Mosaic Virus and Preparation of an Antiserum against the virus

Chen Qijian Zhou Zhongju Lin Qiying Xie Lianghui(Institute of Plant Virology, FAU, Fuzhou 350002)  
Sugarcane mosaic virus was purified from the leaves of the diseased sweet maize inoculated artificially with ScMV-A and used in the experiment. The procedures of purification involved precipitation with PEG differential centrifugation and 10-40% sucrose density gradient centrifugation. The virus preparation was examined with a UV spectrophotometer and showed a typical nucleoprotein spectrum with maximum absorption at 257 nm and minimum at 240 nm, A260/280=1.68. The yield of ScMV ranged from 1.25 to 1.37mg per kilogramme of diseased leaves. The purified ScMV preparation was injected into rabbits to produce an antiserum to ScMV. Titer of the ScMV antiserum was 1/1024 by SPA-ELISA. The ScMV antiserum obtained can also be used to detect ScMV.
【Fund】: 福建省教委资助
【CateGory Index】: S435.661
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【References】
Chinese Journal Full-text Database 6 Hits
1 HUANG Chengmei1?2, LI Yangrui1?2, TAN Yumo3, YE Yanping1 (1. Agricultural College, Guangxi University, Nanning, Guangxi 530005, China; 2. Guangxi Academy of Agricultural sciences, Nanning, Guangxi 530007, China; 3. Guangxi Sugarcane Research Institute, Nanning, Guangxi 530007, China);A brief study on the virus-free techniques and  the detection methods in sugarcane[J];Sugarcane;2002-04
2 JIANG Ke-qing QUE You-xiong XU Li-ping ZHANG Ling-yanKey Lab of Sugarcane Eco-Physiology & Genetic Improvement Ministry of Agriculture Fuzhou 350002 China;Construction of Plasmid Containing Coat Protein Gene of Sugarcane Mosaic Virus Strain E and Prokaryotic Expression of Coat Protein Gene[J];Letters in Biotechnology;2006-02
3 QUE You-Xiong1,JIANG Ke-Qing1,2,XU Li-Ping1,GUO Ying1,LIN Jian-Wei1,CHEN Ru-Kai1 1.Key Lab of Sugarcane Genetic Improvement,Ministry of Agriculture,Fuzhou 350002;2.Life Science College,Sun Yat-Sen University,Guangzhou 510006;China;Purification of Recombinant Coat Protein of Sugarcane Mosaic Virus Strain E and Preparation of its Polyclonal Antibody[J];Letters in Biotechnology;2009-01
4 Wang Wenzhi Ma Ziman Zhang Shuzhen Yang Benpeng Cai Wenwei Gu Lihong Li Jiao (Institue of Tropical Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101);Research on Genetic Engineering of Sugarcane Mosaic Disease[J];Biotechnology Bulletin;2009-01
5 HUANG Ying-kun,LI Wen-feng,LU Wen-jie,LUO Zhi-ming(Sugarcane Research Institute,Yunnan Academy of Agricultural Science,Kaiyuan 661600,China);The Causes of Sugarcane Mosaic Disease Eepidemic in Yunnan Sugarcane Area and the Control Strategy[J];Journal of Yunnan Agricultural University;2007-06
6 LI Li jun, ZHOU Zhong ju, XIE Lian hui ** (Institute of Plant Virology of Fujian Agricultural and Forestry University, Fuzhou 350002, China);Cloning of 3'-terminal Part of Sugarcane Mosaic Virus RNA and Sequence Comparison of CP Gene Between Chinese Isolate and Other Strains of SCMV[J];Virologica Sinica;2001-01
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 Xu Liping;Chen Rukai; Li Yaoping(Synthethic Research Institute of Sugarcane, FAU, Fuzhou 350002);Elimination of Sugarcane Mosaic Virus by Callus Tissue Culture and Apical Culture[J];JOURNAL OF FUJIAN AGRICULTURAL UNIVERSITY;1994-03
2 LIU Li\|hua 1,LIN Qi\|ying 2, XIE Hua\|an 3,XIE Lian\|hui 2 (1 Central Laboratory,Fujian Academy of Agricultural Sciences,Fuzhou 350003,China; 2 Institute of Plant Virology,Fujian Agricultural University; 3 Fujian Academy of Agricultural Sci;Pathogenesis-related proteins and plant disease resistance[J];FUJIAN JOURNAL OF AGRICULTURAL SCIENCES;1999-03
3 ZHANG Wen shui 1, PAN Da ren 2, LIN Yuan quan 2, XU Li ping 2, FU Hua ying 2 (1.Fujian Tobacco Co. Sanming Branch, Sanming, Fujian 365000, China; 2.Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China);Study on eliminating sugarcane mosaic virus (SCMV) by meristem tip of Badila (S.officinarum L.)[J];Sugarcane;2002-02
4 Wei Jiachuan Zhang Huiying Zeng Bilan (Biotechnology Research Centre,Guangxi Univ.,Nanning 530005);Effect of Paclobutrazole and Choline Chloride on Growth of Banan Plantlets In Vitro[J];JOURNAL OF GUANGXI AGRICULTURED AND BIOLOGICAL SCIENCE;1999-01
5 LI Yangrui (Guangxi Academy of Agricultural Sciences, Nanning 530007,China);Discussion about the Sugarcane Sugar Industry Development in Guangxi after China's entrance to WTO[J];Guangxi Agricultural Science;2003-01
6 Miao Hongqin; Di Dianping; Wu Heping(Ins. of Pl. Prot., Hebei Acad. of Agri. and For. Sci., Baeding 071000);A Preliminary Study on Effects of Seed-borne Virus Rate and Resistance Level by Inoculating MDMV in Different Growth Periods[J];JOURNAL OF AGRICULTURAL UNIVERSITY OF HEBEI;1998-01
7 Wu Caiwen; Qin Tinghao; Zhou Zhonglan; Chen Yuanyi(Sugar and Sugar-yielding Plants Ind. Res. Inst. of Sichuan Province, Sichuan 641200);Studies on Rapid Propagation of Sugarcane by Tissue Culture[J];SUGARCANE AND CANESUGAR;1997-05
8 Luo Jun Zhang Muqing Lin Yanquan Lü Jianlin Chen Rukai (The Key Lab of Sugarcane Genetic & Breeding of Ministry of Agriculture, P.R.China, Fuzhou 350002);Chloroplast fluorescence parameters, MDA content and plasma membrane permeability in sugarcane and their relation to drought tolerance[J];JOURNAL OF FUJIAN AGRICULTURAL UNIVERSITY(NATURAL SCIENCE);1999-03
9 Liang He; Li Yangrui; Zhou Shengmao; Man Shizhi; Huang Wengyao(Dept. of Agronomy, Guangxi Agric. Univ., Nanning 530005);Effects of Different Concentrations of Ethephon on Some Physiological and Biochemical Characters in Sugarcane[J];;1995-01
10 Shi Jianxin, An Xiuzhang, Zhang Lixin, Zhao Yingli, and Yan Xiaofang (Institute of Farm Products Storage,Shanxi Academy of Agricultural Sciences, Taiwan 030031 );Effect of Postharvest Illumination on Enhancing the Fruit Red Color and Storage Life of Fuji Apples[J];Journal of Fruit Science;2000-03
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 XIAN Wu,WANG Lun-wang,TANG Shi-yun,LIU Hai-bin(Guangxi Sugarcane Research Institute,Nanning,Guangxi 530007,China);Technique of virus-free meristem tip culture for new sugar cane varieties such as GT21[J];Subtropical Agriculture Research;2007-02
2 CHEN Jiong,CHEN Jian-Ping*(Virology Department,Zhejiang Academy of Agricultural Sciences,Hangzhou310021,China);Sugarcane Mosaic Disease in Zhejiang Province Was Caused by Sorghum Mosaic Virus and Sugarcane Mosaic Virus[J];Chinese Journal of Virology;2002-04
3 ZHOU Min-qiang,YI Dai-yong,BAN Xiu-wen,GONG De-yong,LIU Fan-zhi(Guizhou Institute of Subtropical Crops,Zhenfeng 562200,China);Study on Cultivation Techniques for Virus-free Seedling of Sugarcane[J];Guizhou Agricultural Sciences;2006-01
4 LU Xiang-ling1,LU Feng2,SONG Bo1(1.College of Agronomy,Shenyang Agricultural University,Shengyang 110161,China;2.Crop Research Institute,Liaoning Academy of Agricultural Sciences,Shenyang 110161,China);The Methods of Identifying Resistant Candidate Genes by Homologic Sequences[J];Acta Agriculturae Boreali-Sinica;2007-S2
5 Jiang Junxi Zhou Xueping Hong Jian(Institute of Biotechnology, Zhejiang University, Hangzhou 310029, China; College of Agronomy, Jiangxi Agriculture University, Nanchang 330045, China);Identification of the Viral Pathogen of Sugarcane Mosaic Disease in Hangzhou[J];Journal of Agricultural Biotechnology;2002-04
6 QUE You-Xiong1,JIANG Ke-Qing1,2,XU Li-Ping1,GUO Ying1,LIN Jian-Wei1,CHEN Ru-Kai1 1.Key Lab of Sugarcane Genetic Improvement,Ministry of Agriculture,Fuzhou 350002;2.Life Science College,Sun Yat-Sen University,Guangzhou 510006;China;Purification of Recombinant Coat Protein of Sugarcane Mosaic Virus Strain E and Preparation of its Polyclonal Antibody[J];Letters in Biotechnology;2009-01
7 Wang Wenzhi Ma Ziman Zhang Shuzhen Yang Benpeng Cai Wenwei Gu Lihong Li Jiao (Institue of Tropical Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101);Research on Genetic Engineering of Sugarcane Mosaic Disease[J];Biotechnology Bulletin;2009-01
8 HUANG Ying-kun,LI Wen-feng,LU Wen-jie,LUO Zhi-ming(Sugarcane Research Institute,Yunnan Academy of Agricultural Science,Kaiyuan 661600,China);The Causes of Sugarcane Mosaic Disease Eepidemic in Yunnan Sugarcane Area and the Control Strategy[J];Journal of Yunnan Agricultural University;2007-06
9 ZHOU Guo-hui XU Dong-lin (Plant Virology Laboratory,South China Agricultural University,Guangzhou 510642,China);On sugarcane viral diseases and their controlling[J];Journal of Plant Protection;2005-03
10 ZHANG Xian-yong,CAI Wen-wei,YANG Ben-peng,ZHANG Shu-zhen,WANG Ye-qiao(Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences(CATAS),Haikou 571101);Development of a Multiplex PCR Protocol for the Detection of SCMV and Clavibacter xyli subsp. Xyli,lxx,the Causal Bacterium of Sugarcane RSD[J];Scientia Agricultura Sinica;2008-12
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