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《Hainan Medical Journal》 2009-12
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Effects of terazosin on cell proliferation and apoptosis in PC-3 cell line.

GUO Bin,LU Xiao-gang,ZHONG Dong-liang,et al.Department of Urology,Minimally Invasive Surgery Center,the First Hospital Affiliated to Guangzhou Medical College,Guangzhou 510230,Guangdong,P.R.CHINA  
Objective To investigate the biological efficiency of terazosin on human prostate cancer PC-3 cells.Methods Exponentially growing PC3 cells were exposed to terazosin with 15 μmol/L,25 μmol/L and 50 μmol/L for 24 h and 48h respectively.The cell growth rate,apoptotic rate and cell cycle were measured by MTT and flow cytometry,and the expression of caspase-7 and PARP were analyzed by Western-Blot.Results With the increasing concentration of terazosin and the incubation time,cell growth was inhibited,and number apoptotic cells increased.The apoptosis of PC-3 cells were correlated with PARP degradation,but not with caspase-7 according to Western-Blot analysis.Conclusion There is a positive relation between the cell growth inhibition,cytotoxic activity and the dose of terazosin,and the mechanism of terazosin-induced cell apoptosis in PC-3 cells may be through the Caspase-PARP pathway.
【Fund】: 广东省科学技术厅项目(编号:2005B30701009)
【CateGory Index】: R737.25
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