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《Strait Pharmaceutical Journal》 2005-05
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Analysis of γ-Amino Butyric Acid in Several Corn Medicinal Materials

CHEN Ti-qiang~1,WU Jin-zhong~2,XU Jie~1 (1. Fujian Academy of Agricultural Sciences,Fuzhou 350003;2.Department of TCM Pharmacology,FCTCM 130003,China)  
OBJECTIVE To improve the pretreatment methods of analysis on γ-Aminobutyric acid(GABA). METHODS Using HITACHI 835-50 type Amino Acid Analyier by standard analysis procedure of 72min ), three kinds of pretreatment method were compared: acid hydrolyzation(GB/T 18246-2000), ethanol circumfluence extraction method and the adopted improved method. RESULTS The adopted improved method was preferable, the recovery is increasing respectively 12%~13.76% and 7.00%~7.39% then other common method, and the recovery was validated up to 98.48% by internal standard method.CONCLUSION The analysed result were comparatively perfect with the adopted improved method, for some corn medicinal materials. Among that, the GABA contents in gemmiparous paddy and barley (dry matter) were higher(0.579 mg·g~-1 and 0.523mg·g~-1 respectively), and the following was fermented red rice(0.419 mg·g~-1), saute gemmiparous-paddy and gemmiparous -paddy were lower relatively (0.304 mg·g~-1 and 0.246 mg·g~-1).
【Fund】: 福建省科技重大专项2004YZ02-4
【CateGory Index】: R284;
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