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《Strait Pharmaceutical Journal》 2013-09
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Determination of Ginsenoside Rb_1 in Gypenoside Tablets

CHEN Ying;Fujian Zhong Lu phamaceutical Co. ,Ltd.;  
OBJECTIVE To estabilish an HPLC method for quantitative determining of ginsenoside Rb 1 in Gypenoside Tablets.METHODS Separation was accomplished on the Agilent Extend-C 18 column(5μm,4.6mm × 250mm),with acetonitrile-water as mobile phase by gradient elution.The flow rate was 1.0mL·min-1 and the detection wavelength was set at 203nm.The column temperature was at 35℃.RESULTS The method offered good linearity 0.422 ~ 2.532μg.mL-1 for ginsenoside Rb 1(r = 0.9997).The average recovery rate was 97.56%(RSD = 1.05%,n = 6).CONCLUSION The method was accurate and repeatable,and can be used for quality control of Gypenoside tablets.
【CateGory Index】: R284.1
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