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《Journal of Hebei University(Natural Science Edition)》 2000-04
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Cultivation of Callus Tissue of Glycyrrhiza inflata and the Glycyrrhizic Acid Content Analysis

LIANG Yuling 1,GUAN Yanying 1,YANG Li 2,WANG Shumin 3 (1.Department of Biology, Baoding Normal College, Baoding 071051, China; 2.Strawberry Research Institute of Baoding,Baoding 071051, China; 3.Baoding Hygiene School,Baoding 071051, China)  
Root, hypocotyl and cotyledon of Glycyrrhiza inflata were cultured on MS medium containing different hormones in light or dark, and root was cultured in liquid medium. The effects of hormones on growth of callus and the content of glycyrrhizic acid were different. Firstly the content of glycyrrhizic acid was not direct proportional to the growth of callus. Secondly, callus tissues culturing in light grew quicker than that in dark, but the callus was harder but the glycyrrhizic acid content was lower than that in dark. Thirdly, the root suspended cultures produced a lot of fibrous roots. The glycyrrhizic acid content of root cultures was obviously higher than that of other cultures.In conclusion ,different cultures of Glycyrrhiza inflata had different abilities of producing glycyrrhizic acid, and the root culture was more suitable for synthesis of the acid.
【Fund】: 保定市科委资助项目
【CateGory Index】: Q943
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