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《Journal of Hubei Polytechnic University》 2001-02
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Separation PE from PC by Column Chromatography

LI Wei, SHAO You yuan, HUANG Guang dou (Dep. of Chemical Engin., Hubei Polytechnic Univ., Wuhan 430068, China)  
This paper studies the condition of separating Phosphatidyl Ethanolamine(PE) from Phosphatidyl Cholines(PC) with silica gel absorption column chromatography .By determining the gradient elution process, gradient and flow speed, the PC and PE were separated satisfactorily, the volume of eluent and the time of eluting were reduced greatly. The optimum elution conditions are: flow speed is 40~60 drops/min, gradient is (1∶2)~(2∶1)(methanol /chloroform,V/V) by means of concave gradient elution.
【Fund】: 湖北省教委资助项目 98B0 0 6
【CateGory Index】: TQ028
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