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《Chemistry & Bioengineering》 2007-05
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Spectrophotometric Determination of Gentamycin Sulfate with Eosin Y

HUANG Ya-li,XU Hong(Department of Chemistry,Guiyang Medical College,Guiyang 550004,China)  
The interaction of gentamycin sulfate(GEN) and Eosin Y was studied by spectrophotometry in Britton-Robinson buffer solution(pH=3.03).Eosin Y had a strong absorbance at 545 nm.When GEN was added into Eosin Y solution,the absorbance value at 545 nm decreased.The decrease of absorbance value was linear with the concentration of GEN.Under the optimal conditions,Beer's law was obeyed in the range of GEN concentration of 0~2.4 mg·L-1,with apparent molar absorptivity of 6.4×104 L·mol-1·cm-1.The mothed was applied to determine the gentamycin sulfate injection solution with satisfactory results.
【CateGory Index】: TQ465
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