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《Journal of Hebei Agricultural Sciences》 2002-02
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Application of Biotechnology in Millet

Yuan Jincheng Liu Yinghui Gong Xuechen(Zhangjiakou Agricultural College, Xuanhua 075131)  
Tissue cultrue and trans-gene of millet were reviewed. Millet tissue didn' t need strict medium. Callus can be initiated on MS, N6, L3 medium. The medium was solidified with 0.7% - 0. 8% agar,3% sucrose,and pH 5.8.2,4-D was the most efficient hormone. Subculture of the callus was promoted by IAA,NAA,BA,LBA and so on.Seeds,roots,shoots,immature embryos and in some case immature seeds can be used as explant. pB1121 and pAHC25 plasmid were used in millet gene gun bom-bardmemt. Camv35S and ubiquitin promoter were constructed and comparied. Kanamycin-resistance gene and GUS gene was used as markers gene.
【CateGory Index】: S515
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