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《Acta Agriculturae Boreall—sinica》 2001-02
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Comparative Analysis of Polymorphism of SSRs Detected on Two Gel Electrophoresis Systems

LI Xin hai 1,JIAO Shao jie 2,FU Jun hua 1, ZHANG Shi huang 1,YUAN Li xing 1,LI Ming shun 1 (1 Institute of Crop Breeding and Cultivation,Chinese Academy of Agricultural Sciences,Beijing 100081,China; 2 Institute of Crop Breeding,Heilongjia  
Thirty five SSR primers and 14 maize inbred lines were used to comparatively analyze polymorphic level of SSRs on two gel electrophoresis systems.The results showed that the number of alleles and PIC value over 35 SSR primers were significantly different between two gel systems.Polyacrylamide gel(12%) had higher ability than Metaphor agarose gel(3%) in discriminating amplified fragments.On the other hand,the correlation coefficient of genetic similarities between 14 lines obtained on two gel systems was 0 516,significantly correlated at P0 01 level,indicating that the information explored by two gel systems were relatively similar.Eight SSR primers of 35 primers were identified to have high PIC value and number of alleles over 14 lines on both two gel systems,and could be used for unique DNA fingerprinting of each inbred line tested.Based on the results,3% Metaphor agarose gel was suggested to be used in genetic mapping,while 12% polyacrylamide gel would be used in DNA fingerprinting or genetic variation studies.
【CateGory Index】: Q943
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1 Wang Fengge 1,2 Zhao Jiuran 2* Dai Jingrui 1* Guo Jinglun 2 Yuan Yaping 3 Wang Lu 2 Yi Hongmei 2 Sun Shixian 4 Lv Bo 5 1 College of Agriculture and Biotechnology, China Agriculture University, Beijing, 100094; 2 Maize Research Center, Beijing Academy of Agricultural and Forestry Sciences, Beijing, 100097; 3 Department of Plant Science, Jilin University, Changchun, 130062; 4 The National Service Center of Agricultural Technique Popularization, Agricultural Ministry, Beijing, 100026; 5 Plant Variety Testing Center, Agricultural Ministry, Beijing, 100026;Criteria for the Construction of Maize DNA Fingerprint Database[J];Molecular Plant Breeding;2007-01
2 HAN Yong-liang,CHANG Jin-hua (College of Agronomy,Agricultural University of Hebei,Baoding,Hebei 071001,China);Comparative Analysis of Two Kinds of Methods to Detect SSRs on Polyacrylamide Gel Electrophoresis Systems[J];Rain Fed Crops;2006-03
3 Zhou Yifeng Zhao Fei Ren Sanjuan Bao Jinsong Shu Qingyao Shen Shengquan College of Agriculture and Biotechnology,Zhejiang University,Hangzhou,310029;Rapid Breeding of Indica CMS Line with Intermediate Amylose Content by Using the Wx Gene Microsatellite Molecular Marker[J];Molecular Plant Breeding;2009-06
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1 Yang Jianbo ;et ai (Rice Institute, Anhui Academy of Agricultural Sciences, Hefei 230031);Indentification of Rice Hybrid (Shan You 63) and Its Three Lies by Using Random Amplified Polymorphic DNA[J];Journal of Anhui Agricultural Sciences;1996-03
2 Zhang Peijiang et al (Anhui Academy of Agricultural Sciences, Key Laboratory of Rice Genetics & Breeding Ministry of Agricultural, The P. R. China, Hefei 230031);Genetic Distance Detected with RAPD Markers among Rice Cultivars (Oryza sativa L.) and its Relation to Heterosis[J];Journal of Anhui Agricultural Sciences;2000-06
3 Cai Jian et al (Key Laboratory of Rice Genetics and Breeding of MOA, AAAS, Hefei 230031);Application of AFLP Molecular Marker in Crop Breeding[J];Journal of Anhui Agricultural Sciences;2002-02
4 YANG Miao-xian et al(College of Life Sciences,Zhongkai University of Agriculture and Engineering,Guangzhou,Guangdong 510225);Application of SSR Molecular Markers in Kiwifruit Genetic Diversity[J];Journal of Anhui Agricultural Sciences;2008-32
5 Peng Zebin;Liu Xingzhi (Institute of Crop Breeding and Cultivation, Chinese Academy of Agricultural Sciences,Beijing 100081).;A Review on Maize Germplasm Base of Hybrids in China[J];JOURNAL OF ANHUI AGRICULTURAL SCIENCES;1994-02
6 Yang Jian bo et al (Rice Institute,Anhui Academy of Agricultural Scieces,Hefei 230031);Indentification of Rice Hybrid (xie you 63) and Its Three lines by useing Random Amplified Polymorphic DNA[J];JOURNAL OF ANHUI AGRICULTURAL SCIENCES;1996-04
7 AO Cheng-qi(School of Life and Environmental Sciences, Wenzhou Normal College, Wenzhou 325027, China);MORPHOLOGY OF POLLEN GRAINS IN CAMELLIA UNDER LIGHT MICROSCOPE[J];Journal of Anhui Normal University(Natural Science);2004-03
8 LI Juan,JIANG Chang-jun technology of Anhui Agricultural University, Anhui Province and Ministry of Science and Technology, Hefei 230036);Preliminary Construction of Core Germplasm of Camellia sinensis in China[J];Journal of Anhui Agricultural University;2004-03
9 SONG Feng-shun~1,ZHU Li-wu~1,ZHANG Shui-ming~1,JIA Bing~1,YANG Zhen-jiang~2,HUANG Yong-feng~2 (1.Department of Horticulture,Anhui Agricultural University, Hefei, 230036; 2 Administration Committee,Protection District of Dangshansu Pear Germplasm Resources,Dangshan,235300);RAPD Analysis of the Pear Germplasm Resources in Protection District of Dangshansu Pear[J];Journal of Anhui Agricultural University;2004-03
10 ZHANG Zi-xue (Anhui Technical Teachers College, Anhui Fengyang 233100);The Relationship Between Hot Pepper Cytoplasm Male Sterility and Endogenous Hormones Content in Leaves and Flower Buds[J];Journal of Anhui Agrotechnical;2002-02
【Secondary References】
Chinese Journal Full-text Database 8 Hits
1 SHEN Tong-wei et al (College of Life Science,Anhui Agricultural University,Hefei,Anhui 230036);Studies on Identifying Authenticity and Purity of Corn Variety by Using SSR Markers[J];Journal of Anhui Agricultural Sciences;2009-19
2 Liu Feng 1 Feng Xuemei 1 Zhong Wen 2 Liu Yudong 1 Yin Zujun 1 Han Xiulan 1 Xu Zichu 3 Shen Fafu 1 1 State KeyLaboratoryofCrop Biology,College ofAgronomy,Shandong Agriculture University,Tai'an,271018;2 Shandong Seed Administration Station,Ji'nan,250100;3 Shandong Xin Qiu Seed Science & TechnologyCo.,Ltd.,Dezhou,253200;Screening of SSR Core Primer Pairs for Identificating Cotton Cultivar[J];Molecular Plant Breeding;2009-06
3 CUI Jiang-hui,XUE Wei,DUAN Xi-fei,WANG Jing-hua,CHANG Jin-hua(Agricultural University of Hebei,Baoding 071000,China);Construction of an Amplified Fragment Length Polymorphism Linkage Map of Sorghum[J];Acta Agriculturae Boreali-Sinica;2007-04
4 WANG Xiao-yi1,2,YU Shuan-cang2,ZHANG Feng-lan2,YU Yang-jun2,ZHAO Xiu-yun2,ZHANG De-shuang2 (1.College of Life Science,Capital Normal University,Beijing 100037,China;2.Vegetable Reaserch Center,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097,China);SSR Fingerprinting and Genetic Distinctness of Pak-choi(Brassica rapa L.ssp.chinensis Makino)[J];Acta Agriculturae Boreali-Sinica;2008-05
5 GUAN Qiang1,ZHANG Yue-xue2,XU Xiang-ling1,SUN De-quan2,LI Sui-yan2,LIN Hong2,PAN Li-yan2,MA Yan-hua2(1.Life and Environmental College,Harbin Normal University,Harbin 150080;2.Pratacultural Science Institute,Heilongjiang Academy of Agricultural Sciences,Harbin 150086);Development of DNA Molecular Marker and Several New Types of Molecular Markers[J];Heilongjiang Agricultural Sciences;2008-01
6 FU Yu-qin1,SUI Guang-lei1,JIANG Yuan1,WANG Pi-wu1,GUAN Shu-yan1,LIU Shu-ying1,HU Xin2(1.College of Life Science,Jilin Agricultural University,Changchun 130118,China;2.Institute of the Quartermaster,General Logistics Department of the PLA,Beijing 100010,China);Establishment of DNA Fingerprinting Database of Dominant Middle-late Mature Maize Hybrids in Jilin Province[J];Journal of Jilin University(Science Edition);2008-06
7 Hu Yongsheng 1,2 Mao Shengli 1 Wang Lihao 1 Zhang Baoxi 1 Liu Mingyue 2 ( 1 Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081) ( 2 Horticulture and Landscape College, Hunan Agricultural University, Changsha 410128 );Optimization of SSR System in Pepper[J];Journal of China Capsicum;2007-02
8 SHAO Dan PEI Ying ZHANG Heng-Qing(College of Life Science,Liaoning Normal University,Dalian 116029);cpSSR Analysis of Variation of Genetic Diversity in Temporal Dimension of Natural Population of Pinus koraiensis in Liangshui National Nature Reserve[J];Bulletin of Botanical Research;2007-04
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