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《Acta Agriculturae Boreall—sinica》 2001-02
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Comparative Analysis of Polymorphism of SSRs Detected on Two Gel Electrophoresis Systems

LI Xin hai 1,JIAO Shao jie 2,FU Jun hua 1, ZHANG Shi huang 1,YUAN Li xing 1,LI Ming shun 1 (1 Institute of Crop Breeding and Cultivation,Chinese Academy of Agricultural Sciences,Beijing 100081,China; 2 Institute of Crop Breeding,Heilongjia  
Thirty five SSR primers and 14 maize inbred lines were used to comparatively analyze polymorphic level of SSRs on two gel electrophoresis systems.The results showed that the number of alleles and PIC value over 35 SSR primers were significantly different between two gel systems.Polyacrylamide gel(12%) had higher ability than Metaphor agarose gel(3%) in discriminating amplified fragments.On the other hand,the correlation coefficient of genetic similarities between 14 lines obtained on two gel systems was 0 516,significantly correlated at P0 01 level,indicating that the information explored by two gel systems were relatively similar.Eight SSR primers of 35 primers were identified to have high PIC value and number of alleles over 14 lines on both two gel systems,and could be used for unique DNA fingerprinting of each inbred line tested.Based on the results,3% Metaphor agarose gel was suggested to be used in genetic mapping,while 12% polyacrylamide gel would be used in DNA fingerprinting or genetic variation studies.
【CateGory Index】: Q943
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