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《Acta Agriculturae Boreall—sinica》 2002-01
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Double Color FISH of rRNA Genes in Wheat Relatives of H.villosa and Th.intermedium

PEI Zi you 1,YUAN Wen ye 1,SUN Shan cheng 1,SUN Yu 1, M Tomita 2,Y Yasumuro 2 (1.Crop Genetics Research Institute,Shanxi Academy of Agricultural Sciences,Taiyuan 030031,China; 2.Faculty of Agriculture,Tottori University,Tottori 680-8553,Japa  
Haynaldia villosa(2n=14,VV) and Thinopyrum intermedium (2n=42,E 1E 1E 2E 2 StSt) are important wheat relatives providing useful resistant genes for wheat improvement.In this paper,rRNA genes coding 25S 5 8S 18S rRNA and 5S rRNA are visualized on H.villosa and Th.intermedium chromosomes by double color fluorescence in situ hybridization.The 25S 5 8S 18S rRNA genes are localized on the short arm of 1V chromosome,and 5S rRNA genes are on the short arm of 5V chromosome of H.villosa. On the other hand,three pairs of the 25S 5 8S 18S rRNA genes and four pairs of the 5S rRNA genes are observed on the Th.intermedium chromosomes,in which 2 pairs of chromosomes have both rRNA genes on their short arms.
【Fund】: 山西省农业科学院青年基金资助项目
【CateGory Index】: S188
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