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《Acta Agriculturae Boreali—Sinica》 2003-03
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RAPD Analysis and Taxology Discussion on Cucumber Materials from Different Fountainhead Areas

LIU Dian-lin, YANG Rui-huan, HA Yu-jie, CHEN De-fu, CHEN Xi-wen, XIA Li-xin (Tianjin Cucumber Research Institute,Tianjin 300192,China; Department of Biochemistry and Molecular Biology, Nankai University,Tianjin 300070,China)  
Random amplified Polymorphic DNA(RAPD) was used to analyze the genetic diversity among cucumber inbred line. The genetic of 39 cucumber inbred line was studied. The result showed that 49 of 172 arbitrary primers could amplify clear and repeatable bands. 398 fragments were obtained. Each primer gave 4-12 bands. The length of the band was 0.2-3.5 kb. The genetic distance(D) value between different breeds of cucumber inbred line was 0.064-0.592. The D value was used to construct a dendrogram by UPGMA.
【Key Words】： Cucumber RAPD Genetic diversity Cluster analysis
【Fund】： 天津市重点攻关项目(993122511-2)
【CateGory Index】： S642.2
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