Full-Text Search：
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Acta Agriculturae Boreali-Sinica》 2006-02
Add to Favorite Get Latest Update

RAPD-Based Identification and Cluster Analysis of Soybean Cultivars (Glycine max L.) in Henan

ZHOU Yan-qing,TIAN Miao-miao,BAO Dan,YUAN Bao-jun, YANG Qing-chun, FANG Si-xia, NIU Jing-yuan, LI Min,ING Jian-zhou (College of Life Sciences, Henan Normal University, Xinxiang 453007, China Zhoukou City Institute of Agricultural Science, Zhoukou 466001, China Department of Biotechnology, Henan Agricultural University, Zhengzhou 453000, China Food and Bioengineering Department,Zhengzhou University of Light Industry,Zhengzhou 453000, China)  
Ten soybean cultivars' total DNAs were extracted by CTAB method and used for identification and cluster analysis based on RAPD method. Out of 73 random primers, 12 were selected to generate stable and polymorphic DNA fragments. Total 67 RAPD fragments were amplified, 51 of which were polymorphic,and whose size varied from 0.2 to 5 kb. Each primer could generate 4 to 9 DNA fragments. The percentage of polymorphic loci was 76.16% . The results analyzed by SPSS11.0 version software showed that Jaccard' similarity coefficients ranged from 0.528 to 0.776,whose mean was 0.641 6, indicating that the genetic variations existed among the 10 soybean cultivars. A dendrogram established based on Hierarchical cluster analysis could divid 10 soybean cultivars into 3 groups, among which there were some genetic relations.
【Key Words】： Soybean (Glycine max . L) RAPD Cultivar identification Cluster analysis
【Fund】： 河南省杰出人才创新基金项目(0321001400) 陕西省生物技术省级重点实验室开放基金项目
【CateGory Index】： S565.1
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 7 Hits
1 YAN Xiu-juan et al (Soybean Research Center,Jilin Academy of Agricultural Sciences,Gongzhuling,Jilin 136100);Study on the Present Applied Situation of Molecular Markers in the Breeding of Soybean[J];Journal of Anhui Agricultural Sciences;2008-13
2 DUAN Yong-hong1,2,3,NIU Xi-wu2,LI Su-qing4,SUN Yi1,2(1.Institute of Bio-technology,Shanxi University,Taiyuan 030006,China;2.Shanxi Academy of Agriculture Sciences,Taiyuan 030006,China;3.Shanxi Agricultural University,Taigu 030801,China;4.Department of Geography,Taiyuan Normal University,Taiyuan 030012,China);Genetic Diversity Analysis of Peashrub in China by RAPD[J];Acta Agriculturae Boreali-Sinica;2009-01
3 WANG Min YANG Wan-ming LI Run-zhi DU Wei-jun(College of Agriculture,Shanxi Agricultural University,Taigu,Shanxi 030801);GENETIC DIVERSITY OF SOYBEAN GERMPLASM BY RAPD MARKER[J];Journal of Nuclear Agricultural Sciences;2010-02
4 XU Lei,WANG Xi-dong,ZHANG Hua,SHI Qing-hua(Laboratory of Agricultural Biological Technology,College of Agronomy,Xinjiang Agricultural University,Urumqi,830052);Identification and Cluster Analysis of RAPD-cultivar of Cicerarietinum L.[J];Journal of Xinjiang Agricultural University;2008-01
5 CHEN Xiao-ling1,2, ZHU Zhen-dong1, WANG Xiao-ming1, XIAO Yan-nong2, WU Xiao-fei1 (1Institute of Crop Science, Chinese Academy of Agricultural Sciences/The National Key Facilities for Crop Genetic Resources and Improvement, Beijing 100081; 2College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070);Postulation of Phytophthora Resistance Genes in Soybean Cultivars or Lines[J];Scientia Agricultura Sinica;2008-04
6 SHI Qing-hua,LI Jie,WANG Xi-dong,ZHOU Gui-ling(Key Laboratory of Agricultural Biotechnology,Xinjiang Agricultural University,Urumqi 830052,China);RAPD Polymorphism of Capparis spinosa L.Population from Xinjiang[J];Journal of Xinjiang Agricultural University;2009-04
7 ZHAO Guang-lei1,ZHU Hong-ju1,Liu Chun-jing2,CHEN Rui-ping2,CAO Chun-bao2,TAN Yong-jun2,CHEN Yao-feng1(1 College of Agriculture,Northwest A&F University,Yangling,Shaanxi 712100,China;2 Agriculture Technical Extension Centre,Luntai,Xinjiang 841600,China);Analysis of genetic diversity in cotton varieties of intercropping of apricot and cotton around Tarim Basin[J];Journal of Northwest A & F University(Natural Science Edition);2010-03
【Citations】
Chinese Journal Full-text Database 7 Hits
1 Yang Shaohui 1 Zhang Lijuan 2 Duan Huijun 1 (1.Agronomy College, HAU Baoding 071001; 2.Lanyang Agricultural College, Lanyang 265200);RAPID EXTRACTION OF SOYBEAN DNA FOR PCR FROM DRY SEED[J];Soybean Science;2003-02
2 ZHAO Hongkun LI Qiyun WANG Yumin ZHUANG Bingchang (Jilin Provincial Agrobio_technology Key Open Lab , Gongzhuling 136100);Use of RAPD for Identification of Soybean Cultivars[J];JILIN AGRICULTURAL SCIENCES;1998-04
3 Xia Qingyou\ Zhou Zeyang\ Lu Cheng\ Xiang Zhonghuai(The Key Sericultural Laboratory of Agricultural Ministry, College of Sericulture & Silk, Southwest Agricultural University\ Chongqing\ 400716);MOLECULAR PHYLOGENETIC STUDY ON THE RACIAL DIFFERENTIATION OF BOMBYX MORI BY RANDOM AMPLIFIED POLYMORPHIC DNA(RAPD) MARKERS[J];ACTA ENTOMOLOGICA SINICA;1998-01
4 ZHOU Yan Qing;JING Jian Zhou;LI Zhen Yong;HAO Jian;JIA Jing Fen;ZHANG Bao Hu;HAO Jian Guo College of Life Sciences, Northwest University, Xi'an 710069, China Postdoctoral Programme, Sino-American Biotechnology Company, Luoyang 471003, China Department of Biological and Biotechnology, Tsinghua University, Beijing 100084, China College of Life Sciences, Henan Normal University, Xinxiang 453007, China Wenxian Institute of Agricultural Sciences, Wenxian 454881, China;GENETIC DIVERSITY OF YAM (DIOSCOREA OPPOSITA THUNB) DETECTED BY ISSR MARKERS[J];Acta Biologiae Experimentalis Sinica;2005-04
5 Qiu Lijuan Randall L. Nelson Lila O. VodkinInstitute of Crop Germplasm Resources, CAAS, Beijing 100081, China; USDA Soybean Germplasm Collection, Urbana, 1L 61801, USA, Department of Crop Science, University of Illinois, Urbana, IL 61801, USA;Evaluation of Soybean Germplasm with Random Amplification Polymorphic DNA(RAPD)Markers[J];Acta Agronomica Sinica;1997-04
6 DUAN Hui-jun, ZHANG Cai-ying, ZHANG Li-juan, MA Zhi-ying (College of Agronomy, Agricultural University of Hebei, Baoding 071001, China);Genetic diversity study of soybean germplasm resource in Hebei province by isozyme and RAPD[J];Chinese Journal of Oil Crop Scieves;2003-02
7 Shi Feng 1, Zhang Yaozhou 1,Zen Guangwen 2( 1Institute of Biochemistry,Zhejiang Agricultural University,Hangzhou 310029; 2Department of Biology,Zhejiang Agricultural University,Hangzhou 310029);Study on identification of soybean cultivars using RAPD markers[J];ACTA AGRICULTURAE ZHEJIANGENSIS;1998-04
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 Wu Shuang et al (Rice Research Institute, AAAS, Hefei 230031);Heritable and Correlation Analysis of Several Restore Line's Floral Character[J];Journal of Anhui Agricultural Sciences;2003-02
2 YANG Runfa 1,XIA Zhongyan 2,ZHANG Chuanhai 2 (1.Luan Agricultural School in Anhui,Luan 237158;2.Institute of Agricultural Sciences of Luan District,Anhui Luan 238009);Genetic Claster Analysis of Plant-type Traits of Rice Varieties[J];JOURNAL OF ANHUI AGROTECHNICAL;2000-01
3 LI Xue-ping~(1,2);HE Zheng-quan~2; CHEN Fa-ju~(1,2);LIANG Hong-wei~2;LI Feng-lan~1.1 College of Biological Science and Biotechnology,Beijing Forestry University,100083,P.R.China;2 Biotechnology Research Center,China Three Gorges University,Yichang,Hubei Province,443002,P.R.China.;RAPD analysis for the genetic diversity of four populations of Davidia involucrata Baill.in Shennongjia area,Hubei Province.[J];Journal of Beijing Forestry University;2006-03
4 CHEN Xue zhen 1, XIE Hao 1, LI li 1, QUI Li Juan 2 ( 1Beijing Agricultural College, Beijing China 102206; 2ICGR,CAAS,Beijing China 100081);Relationship of the Agronomic Character in F_2 Generation of Soybean[J];Journal of Beijing Agricultural College;2002-04
5 LI Shu-chen,CHEN Xue-zhen,XIE Hao,WANG Jian-chen,WANG Wei (Beijing Agricultural College, Beijing 102206);Analysis and Comparison of Agronomic Characters in New Soybean Lines[J];Journal of Beijing Agricultural College;2004-02
6 SONG Jun-shuang, WANG Zan, GAO Hong-wen (Institute of Animal Science, Chinese Academy of Agriculture Science, Beijing 100094,China);ISSR Analysis on the Genetic Diversity of Three Caragana Fabr. Species[J];Acta Agrestia Sinica;2006-04
7 XUE Chong-yun, HE Wen-ming, ZHANG Rui-ling ( China Academy of Pulping and Papermaking, Beijing 100020, China);The feasibility study of pulping and papermaking with hybridized Chinese pennisetum[J];China Pulp & Paper Industry;2006-01
8 ZHANG Cai ying, DUAN Hui jun, ZHANG Li juan ,SUN Hui xian(Agricultural University of Hebei, Baoding 071001,China);The studies on agronomic traits of soybean varieties and their relations from different growing areas[J];JOURNAL OF AGRICULTURAL UNIVERSITY OF HEBEI;2000-02
9 LI Yong jun 1 SU Jia kai 2 (1. The Center of Preservation and Utilization of Germplasm Resources of Animal Husbandry and Forage Grass, The Center of National Animal Husbandry & Veterinary Service, Beijing 100094; 2. Institute of Animal Science, CA;Study on relationships of the alfalfa local varieties in China Ⅱ RAPD markers[J];ACTA PRATACULTURAL SCIENCE;1999-03
10 WANG En-hua, LIU Jie, LIU Gong-she,QI Dong-mei, LI Fang-fang (Institute of Botany, The Chinese Academy of Sciences, Beijing 100093, China);Germplasm identification and genetic evaluation on Leymus chinensis with morphology and molecular marker[J];Acta Pratacultural Science;2002-04
【Secondary References】
Chinese Journal Full-text Database 3 Hits
1 WANG Xian-sheng1, JIA Yu-ying1, GU Han-yan1, HE Xiao-ling1, ZHANG Hua2, ZHANG Ju-song2, SHI Shu-bing2, LI Jian-gui2*, MA Hao1* (1.State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China; 2.Key Laboratory of Agriculture Biotechnology, Xinjiang Agricultural University, Urumqi 830052, China);Information analysis of EST resource of chickpea[J];Journal of Hunan Agricultural University(Natural Sciences);2010-02
2 SUI Zhe,HUANG Jing,MA Zhen-chuan,SHI Xiao-lu,XIE Jing,WEN Jing-zhi(Department of Plant Protection,College of Agriculture,Northeast Agricultural University,Harbin 150030,China);Evaluation of resistant genes to phytophthora root and stem rot in soybean from Jilin and Liaoning provinces[J];Chinese Journal of Oil Crop Sciences;2010-01
3 TANG Qing-hua1,CUI Lin-kai1,LI De-long1,DAI Ting-ting1,YIN Wei-xiao1,DONG Suo-meng1,XING Han2,ZHENG Xiao-bo1,WANG Yuan-chao1 (1College of Plant Protection,Nanjing Agricultural University/Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects,Ministry of Agriculture,Nanjing 210095;2National Center for Soybean Improvement/National Key Laboratory for Crop Genetics and Germplasm Enhancement,Nanjing 210095);Resistance Evaluation of Soybean Germplasm from Huanghuai Valley to Phytophthora Root Rot[J];Scientia Agricultura Sinica;2010-11
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Lu Cuihua He Zhihong Song Yingshu Xue Qingxi (Soybean Research Institute of Heilang Jiang Academy of Agr. Sei. );ISOZYME TYPE ANALYSIS OF SOYBEAN PLANTED IN HEILONGJIANG PROVINCE[J];Soybean Science;1990-02
2 Zhong Zhenping Liu Dejing Chen Qifeng(Dept. of agronomy, Fujian Agricultural College; Institute of Genetics and Crop Breeding , Fujian Agricultural College);ISOZYMES COMPARISION AMONG SOYBEAN SPECIES[J];Soybean Science;1992-04
3 Zhou Sijun (Biotechnology Research Center, Heilongjiang Academy of Agricultural Sciences,Harbin,150086);RAPID ISOLATION OF SOYBEAN DNA FOR PCR WITH LARGE NUMBERS OF SMALL SAMPLES[J];SOYBEAN SCIENCE;1999-04
4 Tian Qingzhen 1 Gai Junyi 1** Yu Deyue 1 Jia Jizeng 2 (1. Soybean Research Institute, Nanjing Agriculture University; National Center of Soybean Improvement, Ministry of Agriculture, Nanjing, 210095; 2. Institute of Crop Germplasm Resource;A STUDY ON AMPLIFIED FRAGMENT LENGTH POLYMORPHISM (AFLP) IN SOYBEAN [J];Soybean Science;2000-03
5 Li Xichen;Lei Bojun;Lu Cuihua;Qian Hua;Zhou Sijun;Lu Yunbo(Bio.Res.Center,Heilongjiang Academy of Agri.Sci.,Harbin,150086)Xie Weiwu;Wang Bin(Institute of Generics,Academia Sinica,Beijing,100101);MOLECULAR VERIFICATION OF SOYBEAN FOREIGN PEA INTRODOCNON TO INDUCE EARLINESS THROUGH RAPD TECHNIQUE[J];SOYBEAN SCIENCE;1994-02
6 Yan Qingshang; Wang Lan; Li Ying; Wang Lianzheng; Chen Pinshan(Institute of Crop Breeding and Cultivation,CAAS, Beijing 100081);PRILIMINARY STUDY ON IDENTIFICATION OF RAPD MARKER ASSOCIATED WITH RESISTANCE TO RACE 4 OF HETERODFRA GLYCINES[J];SOYBEAN SCIENCE;1996-02
7 Zhang Zhiyong Chen Shouyi (Institute of Genetics, Academia Sinica, 100101) Gai Junyi Hu Yunzhu Zhi Haijian (Soybean Research Institute, Nanjing Agricultural University,210095);POLYMORPHISMS AND CLUSTER ANALYSIS OF CULTIVATED SOYBEANS WITH RAPD[J];SOYBEAN SCIENCE;1998-01
8 LIU Zhao jun (Biotechnology Restarch Center, Heilongjiang Academy of Agri. Sci, Harbin, 150086);Application of Isozymes Analysis Technique in Soybean Breeding[J];Heilongjiang Agricultural Science;2001-06
9 Su qiao 1 Liu Wen-zhe 1 Wu jun 1 An Li-jia 1 Sun Bai-zeng 2(1. College of Life Sciences, Liaoning Noraml University, Dalian 116029) (2. Agriculture Science Institute of Tieling, Tieling 112616);RAPD ANALYSIS OF NORTHEAST CHINA SOYBEAN GENOTYPES[J];;1998-02
10 Qiu Yingxiong Fu Chengxin Kong Hanghui ( College of Life Sciences, Zhejiang University, Hangzhou 310029, China);Inter-simple Sequence Repeat(ISSR) Analysis of Different Cultivars in Myrica rubra[J];Journal of Agricultural Biotechnology;2002-04
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved