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《Acta Agriculturae Boreali-Sinica》 2008-S1
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Studies on Suitable Factors for Callus Formation and Differentiation of Mature Embryo in Winter Wheat with High Efficiency

LONG Su-xia,ZHAO Fang-hua,XU Zhen-long,XIAO Kai(College of Agronomy,Agricultural University of Hebei,Baoding 071001,China)  
Using the mature embryos of four winter wheat cultivars to be the explants,the effects of pre-treatments of temperature,2,4-D concentrations in callus formation medium,and phytohormone types and their combination in callus differentiation medium on callus formation and differentiation were studied.The results showed that low temperature(4℃) pre-treatment could obviously improve the callus formation rate compared to room temperature pretreatment(25℃).Suitable 2,4-D concentration for callus formation in the callus induction medium was 4 mg/L.The combination of phytohormones of 1 mg/L KT +2 mg/L 6-BA had higher the callus differentiation rate than those in 1 mg/L KT or in 2 mg/L 6-BA,the single type of phytohormone treatment.Under the same culture condition,there were dramatic differences on callus formation and differentiation among the tested cultivars.Thus,low temperature pretreatment,using suitable 2,4-D concentration during the callus induction process,combining the suitable phytohormone combination in callus differentiation are important factors,to improve the callus formation and differentiation,when using the mature embryo to be the plant regeneration explants.
【Fund】: 国家重点基础研究发展计划(“973”计划)前期专项资助(2007CB116209)
【CateGory Index】: S512.1
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【References】
Chinese Journal Full-text Database 7 Hits
1 HE Jie et al(Henan Science and Technology College,Xinxiang,Henan 453003);Research on the Factor Affecting the Regeneration System from Wheat Mature Embryo Culturing[J];Journal of Anhui Agricultural Sciences;2010-15
2 JIN Zhen-lan et al(College of Agronomy,Yanbian University,Longjing,Jilin 133400);Effects of Different Medium on Callus Induction from Taxus cuspidate Sied. et Zucc.[J];Journal of Anhui Agricultural Sciences;2010-19
3 WANG Xing-wen et al(Department of Biological and Environment Engineering,Hefei University,Hefei,Anhui 230022);The Effect of Pretreatment of Stover on the L-lactic Acid Production by Rhizopus oryzae[J];Journal of Anhui Agricultural Sciences;2011-08
4 ZHANG Jia et al(Department of Agronomy,Yanbian University,Yanji,Jilin 133002);Optimization of Inductive Callus Medium for Schisandga chinensis Baill by Uniform Design[J];Journal of Anhui Agricultural Sciences;2011-12
5 ZHANG Hong-mei et al(College of Life Science,Hebei United University,Tangshan,Hebei 063000);Effects of Different Culture Conditions on Callus Ephedrine Production in Ephedrae sinica Stapf[J];Journal of Anhui Agricultural Sciences;2012-05
6 GUO Li1,GU Jun-tao2,LONG Su-xia1,GUO Cheng-jin1,XIAO Kai1(1.Colloege of Agronomy,Baoding 071001,China;2.College of Life Science,Baoding 071001,China);Studies on Wheat Genetic Transformation Mediated Via Agrobacterium-tumefaciens in Which the Mature Embryos to be Explants[J];Acta Agriculturae Boreali-Sinica;2009-S1
7 AN Hui-ling,XU Xiao-jing,ZHANG Jin,WEI Shan-jun,ZHOU Yi-jun (Colleage of Life and Environmental Sciences,Minzu University of China,Beijing 100081,China);Callus Induction and Culture Characteristics of Suspension Cells of Thellungiella halophila[J];Journal of Henan Agricultural Sciences;2013-06
【Citations】
Chinese Journal Full-text Database 5 Hits
1 LIN Yi1,GAO Junshan1,LI Yan2(1.College of Life Science, Anhui Agricultural University;2.College of Life Science,University of Science & Technology of China);Effect of Different Media on Regeneration of Immature Embryo of Wheat (Triticum aestivum)[J];Journal of Anhui Agricultural University;2003-01
2 LIN De-shu~1, WANG Yan-li~2, ZHUAN Zhen-hong~1, LU Guo-dong~3, YE Xing-guo~2, WANG Zong-hua~1,3 (1.College of Life Science, Fujian Agriculture and Foresty Universti y, Fuz hou, Fujian 350002, China;2.Institute of Crop Science, Chin e se Academy of Agricultural Sciences, Beijing 100081, China;3.Key Lab oratory for Biopesticide and Chemical Biology of Ministry of Education, Fujian A griculture and Forestry University, Fuzhou, Fujian 350002, China);A preliminary study of regeneration system of wheat mature embryo and its particle biolistic transformation[J];;2005-02
3 QIN Jian-bing~(1,2),WANG Yue-sheng~1,HE Guang-yuan~1(1.School of Life Science and Technology,Huazhong University of Science and Technology,Wuhan 430074;2.School of Biological Engineering,Fujian Normal University,Fuzuo 350007);Study on plant hormone affecting the in vitro culture of inflorescence of wheat(Triticum aestivum L.)[J];Journal of Central China Normal University(Natural Sciences);2005-03
4 YU Guirong, YIN Jun, GUO Tiancai, NIU Jishan(National Wheat Engineering Technology Research Center for Wheat, Zhengzhou, Henan 450002, China);Selection of the Optimum Genotype for Immature Embryo Culture of Wheat[J];Acta Tritical Crops;2003-02
5 LI Gen-ying~(1,2,3),HUANG Cheng-yan~3,SUI Xin-xia~3,HE Zhong-hu~(2,4),SUN Qi-xin~1,XIA Xian-chun~2(1.College of Agronomy & Biotechnology,China Agricultural University,Beijing 100094,China;2.National Wheat Improvement Center/Institute of Crop Science,Chinese Academy of Agricultural Sciences,Beijing 100081,China;3.Crop Research Institute,Shandong Academy of Agricultural Sciences,Jinan,Shandong 250100,China;4.CIMMYT China Office,Beijing 10081,China);Tissue Culture Efficiency of Different Explants from Wheat[J];Journal of Triticeae Crops;2006-01
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 Tang Ning(The Department of Life Science,Nanjing Xiaozhuang College,Nanjing,Jiangsu,211171);Studies on Radiation Breeding of Atractylodes Macrocephala Koidz[J];Anhui Agricultural Science Bulletin;2007-23
2 Mao Yanzhi(Potato Research Institute;Heilongjiang Academy of Agricultural Sciences;Keshan;Heilongjiang 161606);Rearch Progress of Transgene CP Potato Breeding for Resisting against Viruses[J];Anhui Agricultural Science Bulletin;2008-03
3 HE Jie et al(Henna Institute of Science and Technology,Xinxiang,Henan 453003);Study on Embryogenic Callus Induction and Plant Regeneration from Mature Embryo of Wheat[J];Journal of Anhui Agricultural Sciences;2007-05
4 YE Jian et al(Key Laboratory of Plant Genetics and Breeding,Sichuan Agriculture University,Yaan,Sichuan 625014);Selection of Transgenic Recipients from Elite Wheat Cultivar and Study on the Half Mature Embryo Culture[J];Journal of Anhui Agricultural Sciences;2007-12
5 WANG Xin-guo et al(Henan Agricultural University/Chinese National Engineering Research Center for Wheat,Zhengzhou,Henan 450002);Effect of Different Media and Hormone Proportioning on the In Vitro Culture of Mature Wheat Embryo[J];Journal of Anhui Agricultural Sciences;2008-06
6 WU Mei-jin et al (Anhui Agricultural University,Hefei,Anhui 230036);Studies on Optimizing the Culture Condition of Wheat Mature Embryo in vitro[J];Journal of Anhui Agricultural Sciences;2008-16
7 GUI Meng-yuan et al(Henan Normal University,Xinxiang,Henan 453007);Induction and Differentiation of Calli from Mature Wheat Embryos[J];Journal of Anhui Agricultural Sciences;2009-23
8 LIU Cai-ping et al(Breeding Center of Tropical Forestry Institute,CAF,Guangzhou,Guangdong 510520);Analysis of the Optimized Factors on the Efficiency of Agrobacterium Mediated Gene Transformation[J];Journal of Anhui Agricultural Sciences;2010-03
9 XIAO Jing et al(Institute of Crop Germplasm Resources,Xinjiang Academy of Agricultural Sciences,Urumchi,Xinjiang 830091);Application of Anther-Pollen Culture Technology in Wheat Breeding[J];Journal of Anhui Agricultural Sciences;2010-17
10 LI Jing-qi et al(Shaanxi Institute of Education,Xi'an,Shaanxi 710061);Study of Dedifferentiation Characteristics of Immature Embryos from Twelve Kinds of Wheat(Triticum aestivum L.) Varieties in the Middle of Guanzhong Irrigated District of Shaanxi Province[J];Journal of Anhui Agricultural Sciences;2012-18
China Proceedings of conference Full-text Database 4 Hits
1 Wang Piwu Wang Lei (Biotechnology Center of jilin Agricultural University, Changchun 130118, China);Optimum Conditions of Callus Induction from Immature Embryosin Maize Inbred Line 7922[A];[C];2002
2 LU Rui-ju~(1,2) SUN Yue-fang~(1,2) WANG Yi-fei~(1,2) SHAN Li-li~(1,2) HUANG Jian-hua~(1,2*) 1.Bioteeh Research Institute,Shanghai Academy of Agricultural Sciences,Shanghai 201106,China; 2.Shanghai Key Lab of Agricultural Genetics and Breeding,Shanghai 201106,China;A study on improving responses of in vitro culture of anther and isolated microspore in japonica rice (Oryza sativa L.)[A];[C];2007
3 Liu Yanzhi;Li Junbo;Wang Yumin;Wang Zhongwei;Ji Feng;Dong Yingshan Jilin Province Key Open Lab. On Agro-biotechnology, Jilin Academy of Agricultural Sciences, Gongzhuling 136100;Establishment of high efficiency donee system in rice genetic transformation[A];[C];2003
4 LIU Tai-guo ZHANG Wen-wei WU Mao-sen LI Shi-fang XIAO Hong CHENG Zhuo-min (Slate Key Lab for Plant Diseases and Insect Pests, Institute of Plant Protection, CAAS, Beijing 100094);Callus Induction Effects of Mature Embryos on Different Wheat Cultivars by Phytohormones[A];[C];2005
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 Yu Hongxin,Liu Jianjun,Feng Zhaoli & Dong Jinduo (Shandong Cadres College of Agricultural Administration,Jinan 250100);Study on Introduction of Vermin-Resistance Gene(CpTI) into Wheat through Pollen-Tube Pathway Method[J];SHANDONG AGRICULTURAL SCIENCES;1999-01
2 HE Jie et al(Henna Institute of Science and Technology,Xinxiang,Henan 453003);Study on Embryogenic Callus Induction and Plant Regeneration from Mature Embryo of Wheat[J];Journal of Anhui Agricultural Sciences;2007-05
3 CAI Xiao-ning et al (Department of Life Science,Nanjing Xiaozhuang College,Nanjing,Jiangsu 211171);Study on the Plant Regeneration in Tissue Culture of Thellungiella halophila Leaf[J];Journal of Anhui Agricultural Sciences;2008-01
4 GUO Xiao-li et al (Department of Life Science of Hengshui College,Hengshui,Hebei 053000);Study on the Culture of Callus from Mature Embryos of Different Wheat Varieties[J];Journal of Anhui Agricultural Sciences;2008-01
5 WU Mei-jin et al (Anhui Agricultural University,Hefei,Anhui 230036);Studies on Optimizing the Culture Condition of Wheat Mature Embryo in vitro[J];Journal of Anhui Agricultural Sciences;2008-16
6 QU Er-jun et al(Department of Bioengineering,Pingdingshan Institute of Technology,Pingdingshan,Henan 467044);Study on the Effect of Hormone on Inducing Leek Callus[J];Journal of Anhui Agricultural Sciences;2008-18
7 ZHANG Hong-mei et al(Department of Biological Science,North China Coal Medical College,Tangshan,Hebei 063000);Effects of Different Hormone Combinations on Callus Induction of Ephedrae sinica Stapf[J];Journal of Anhui Agricultural Sciences;2009-20
8 WANG Nan et al (College of Traditional Chinese Medicine,Jilin Agriculture University,Changchun,Jilin 130118);Study on Condition of Callus Induction of Asarum[J];Journal of Anhui Agricultural Sciences;2010-05
9 LIN Yi1,GAO Junshan1,LI Yan2(1.College of Life Science, Anhui Agricultural University;2.College of Life Science,University of Science & Technology of China);Effect of Different Media on Regeneration of Immature Embryo of Wheat (Triticum aestivum)[J];Journal of Anhui Agricultural University;2003-01
10 HE Jie ZHAO Jun-jie HU Hai-yan ZHOU Yan(Henan Institute of Science and Technology,Xinxiang 453003);Callus Induction and Plant Regeneration System of Wheat Mature Embryo[J];Journal of Chongqing University of Science and Technology(Natural Sciences Edition);2009-05
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 HE Jie et al(Henan Science and Technology College,Xinxiang,Henan 453003);Research on the Factor Affecting the Regeneration System from Wheat Mature Embryo Culturing[J];Journal of Anhui Agricultural Sciences;2010-15
2 AI Jun,WANG Zhenxing,QIN Hongyan,XU Peilei,LIU Yingxue,FAN Shutian,SHEN Yujie(Institute of Special Wild Economic Animal and Plant Science,Chinese Academy of Agricultural Sciences,Changchun 130112,China);Status and prospect of Schisandra chinensis (Turcz.) Baill[J];Journal of Northeast Agricultural University;2012-10
3 JIN Ling-ling et al(Agriculture College,Yanbian University,Yanbian,Jilin 133002);Study on Rooting in vitro of Phellodendron amurense[J];Journal of Anhui Agricultural Sciences;2013-04
4 Gao Xingying Li Meilan Wang Tingting Yin Lihui Hou Leiping * College of Horticulture, Shanxi Agriculture University, Taigu, 030801;Effects of Antibiotics on Plant Regeneration on Chinese Chive (Allium tuberosum Rottle) Callus[J];Molecular Plant Breeding;2013-04
5 GUO Yan-jun;JI Qian-hua;Department of Biology,Fruit Tree Research Institute,Zhaoqing University;;Study on the Establishment of Regeneration System of Poncirus trifoliate(L.) Raf by Uniform Design[J];Journal of Anhui Agricultural Sciences;2014-21
6 ZHONG Ming,HUANG Guo-tao,BAI Li-ping,ZHANG Li,MA Hui,ZHANG Li-jun,GUO Zhi-fu(Key Laboratory of Agricultural Biotechnology of Liaoning Province,College of Biology Science and Technology,Shenyang Agricultural University,Shenyang 110866,China);New Strategy to Reconstruct Agrobacterium-mediated Plant Transgenic Expression Vectors[J];Acta Agriculturae Boreali-Sinica;2011-01
7 ZHANG Dong-wu1,LIU Hui1,ZHAO Hui-xian1,2(1.College of Life Sciences,Northwest A&F University,Shaanxi Yangling 712100,China;2.State key laboratory of crop stress biology for arid areas(NWAFU),Yangling Shaanxi 712100,China);Optimization of Regeneration System of Tissue Culture from Mature Embryos and Screening of Wheat Genotypes with High Regeneration Frequency[J];Journal of Triticeae Crops;2011-05
8 LI Wei-yan,LIU Xin-lun,CHEN Chun-huan,JI Wan-quan(College of Agronomy,Northwest A & F University,Yangling,Shaanxi 712100,China);Study on the Conditions of Wheat Embryonic Callus Induction from Mature Embryos[J];Journal of Triticeae Crops;2011-06
9 CHEN Jun-nan,DENG Zhi-ying,TIAN Ji-chun(State Key Laboratory of Crop Biology,Shandong Key Laboratory of Crop Biology, Shandong Agricultural University,Taian 271018,China);Optimization of Regeneration Culture System from Mature Embryos and Its Influencing Factors[J];Journal of Triticeae Crops;2012-02
10 SONG Cheng-li1,WANG Xuan2,4*,XU Hong1,LIU Ying-tuan1,ZHANG Gai-sheng2,3,WANG Jun-wei2,3(1.College of Life Science,Northwest A&F University,Yangling,Shaanxi 712100,China;2 College of Agronomy,Northwest A&F University,Yangling,Shaanxi 712100,China;3.Key Labtorary of Crop Heterosis of Shaanxi Province,Yangling, Shaanxi 712100,China;4.Department of Biological Engineering,Xi'an Vocational and Technical College,Xi'an,Shaanxi 710077,China);The Influencing Factors of Agrobacterium Tumefaciens Infecting Mature Embryo of Wheat(Triticum aestivum)[J];Journal of Triticeae Crops;2012-02
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 LIN Yi1,GAO Junshan1,LI Yan2(1.College of Life Science, Anhui Agricultural University;2.College of Life Science,University of Science & Technology of China);Effect of Different Media on Regeneration of Immature Embryo of Wheat (Triticum aestivum)[J];Journal of Anhui Agricultural University;2003-01
2 MING Feng 2,LI Wen xiong 1,HU Shang lian 1 (1Northeast Agricultural University,Harbin Heilongjiang 150030 PRC) (2China Agricultural University,Beijing,100094 PRC);The study on the factors affecting the generation of embryogenic callus in wheat[J];JOURNAL OF NORTHEAST AGRICULTURAL UNIVERSITY;2000-01
3 YIN Jun,REN Jiangping,SONG Li,LI Lei,SHI Xuezhen,GUO Xiangyun(National Engineering Research for Wheat,Henan Agricultural University,Zhengzhou 450002,China);Regeneration culture from bombarded immature embryos of wheats and transgenic plant[J];Acta Botanica Boreali-occidentalia Sinica;2003-09
4 YANG Shu-shen , SHAN Lun, XU Hong, ZHANG Gui-cheng(College of Life Science, Northwest Sci-Tech University of Agriculture and Forestry, Yangling Shaanxi 712100,China);The callus induction and plant regeneration of wheat mature embryos under osmotic stress[J];Acta Botanica Boreali-occidentalia Sinica;2003-12
5 Liao Xiangru1 Du Jianfang1 Wang Junxia2 Wang Junli1 Song Luhua1 Chen Piling2 Zhu Baocheng2( 1 Research Center of Biotechnology,Hebei University, Baoding 071002; 2 Department of Biology,Hebei University, Baoding 071002);Effect of Seed Pretreatment on Callus Formation of Wheat Mature Embryo[J];JOURNAL OF HEBEI UNIVERSITY(NATURAL SCIENCE EDITION);1999-01
6 Wang Pei Pei Cuijuan Chen Yurong(Cereal and oil Crops Institute, Hebei Academy of Agricultural and Foresty Sciences, S h ijiazhuang);The Response of Different Inflorescences Section of Winter Wheat at Distinct Development Stages to In vitro Culture[J];Acta Agriculturae Boreali—Sinica;1990-01
7 Qin Jianbing He Guangyuan (Huazhong University of Science and Technology, Wuhan 430074,China);Preliminary Study on in vitro Culture of Different Wheat Genotypes and Their Explants[J];Journal of Huazhong Agricultural;2001-06
8 WU Bi hua 1, ZHENG You liang 1, ZHOU Yong hong 1, YANG Rui wu 2 (1. Triticeae Research Institute, Sichuan Agricultural University, Dujiangyan City, Sichuan 611830, China; 2. Department of Basic Sciences, Sichuan Agricultural University, Yaan Ci;Study of Dedifferentiation Characteristics from Immature Wheat Embryos in Sichuan[J];Acta Tritical Crops;2001-02
9 Xiao Hailin Zhao Shixu;STUDY ON SEVERAL IMPORTANT FACTORS AFFECTING THE CULTURE IN VITRO OF YOUNG SPIKE OF WHEAT[J];;1989-02
10 Xiao Xingguo; Zhang Aimin; Nie Xiuling (Key-Laboratory of Plant Physiology & Biochemistry The Ministry of Agriculture of China Agricultural University; Department of Plant Genetics and Breeding, China Agricultural University Beijing 100094 China );Wheat Genetic Transformation: Advances and Prospects[J];JOURNAL OF AGRICULTURAL BIOTECHNOLOGY;2000-02
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