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《Acta Agriculturae Boreali-Sinica》 2008-S1
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Effects of Different Pretreatments on Callus Induction from Anther of Chinese Jujube

WU Xiao-hong1,WANG Jiu-rui2,LIU Meng-jun1,DAI Li1(1.Research Center of Chinese Jujube,Agricultural University of Hebei,Baoding 071001,China;2.College of Forestry,Agricultural University of Hebei,Baoding 071001,China)  
Five cultivars of Chinese jujube(Ziziphus jujuba Mill.),including Zanhuangdazao,Dongzao,Yueguangzao,Fupingdazao and Lajiaozao,were used to study the effects of different pretreatments on callus induction from anthers.The results showed that both concentration and treatment period of mannitol significantly influnced the callus fomation;The optimal treatment combinations of mannitol for cultivar Zanhuangdazao and Fupingdazao were both dipping anthers in 0.1-0.5 mol/L mannitol for 3 d,with highest callus rate of 98.89% and 82.22%,respectively;Longer period of mannitol treatment decreased the callus rate;The callus rate was not obviously correlated with browning rate.Low temperature(4℃) pretreatment did not significantly accelerate the callus induction of anther,and in case of cultivar Dongzao and Yueguangzao callus rates were significantly reduced.The effects of high temperature(35℃) pretreatment on callus induction rate(increasing,decreasing,or keeping stable) changed with cultivars.
【Fund】: 国家科技支撑计划;; 河北省自然科学基金(C2007000452);; 河北省科技支撑计划;; 河北农业大学科技将帅项目;; 河北农业大学9816项目
【CateGory Index】: S665.1
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