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《Acta Agriculturae Boreali-Sinica》 2009-03
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Cloning and Structure Analysis of Foxtail Millet APDH Gene

CUI Run-li2,4,WANG Yong-fang1,ZHI Hui1,3,LI Wei1,LI Hai-quan1,HUANG Zhan-jing4,DIAO Xian-min1,3(1.Institute of Millet Crops,Hebei Academy of Agriculture and Forestry Sciences,Shijiazhuang 050031,China;2.Department of Pharmaceutical Engineering,Hebei Chemical Medicine Vocational and Technical College,Shijiazhuang 050026,China;3.Institute of Crop Sciences,Chinese Academy of Agriculture Sciences,Beijing 100081,China;4.College of Life Sciences,Hebei Normal University,Shijiazhuang 050012,China)  
It was found that glyceralehyde-3-phosphate dehydrogenase(GAPDH)was frequently up-rgulated expression in salt and drought stress treatment experiments of foxtail millet seedlings in our previous work and from literature.For detail annotation of the function of GAPDH in tolerance reaction of abiotic stress,full length cDNA of foxtail millet GAPDH gene was cloned using RACE technology in this paper.This full length cDNA contains a 1 008 bp open-reading-frame which encoding a 338 amino acid peptide with an estimated molecular weight of 35 911.03 Da.Sequence comparison and phylogenetic analysis indicated that the foxtail millet GAPDH cDNA was highly identical with its ortholog from maize and the next most similar orthologs come from two other cereals,rice and wheat.The possible functions and related topics were discussed.
【Fund】: 国家自然科学基金(30630045);; 国家“863”计划(2006AA10Z1572008AA10Z158);; 国家支撑计划(2006BAD02B02)
【CateGory Index】: S515
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