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《Acta Agriculturae Boreali-Sinica》 2009-04
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Peach SRAP System Optimization and SSR Peach Varieties in the Comparative Identification

GUO Rui1,LI Xiao-yan1,WANG Li-rong2,WANG Guo-shan3(1.College of Agronomy,InnerMongolia Agricultural Institute,Huhhot 010019,China;2.Chinese Academy of Agricultural Sciences Zhengzhou Fruit Tree Institute,Zhengzhou 450093,China;3.The Foodstuff Production Base of Military District in Inner Mongolia,Huhhot 010010,China)  
The Peach some varieties were used to adopt uniform design,the SRAP-PCR reaction system Taq DNA polymerase,the template DNA,dNTPs,Mg2+,primers five components to optimize the concentration.The results showed that 10 μ L Peach SRAP reaction system of the best components,including 2.5 μL 10×PCR Buffer+Mg2+,1 U Taq DNA polymerase,40 ng template DNA,0.2 mmol/L dNTPs,0.2 μmol/L primer.Established by the use of the system to other parts of peach germplasm to amplify the results of clear and reliable,good polymorphism.And using random selection of the SRAP primer and SSR primer respectively distinction between identification of eight kinds of peach germplasm and found that SRAP can be used to separate peach germplasm,and the SSR was used to separate,that the peach variety identification on the SRAP in more than SSR Convenient,and relatively simple operation,the applicability.
【CateGory Index】: S662.1
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