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《Acta Agriculturae Boreali-Sinica》 2009-04
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Peach SRAP System Optimization and SSR Peach Varieties in the Comparative Identification

GUO Rui1,LI Xiao-yan1,WANG Li-rong2,WANG Guo-shan3(1.College of Agronomy,InnerMongolia Agricultural Institute,Huhhot 010019,China;2.Chinese Academy of Agricultural Sciences Zhengzhou Fruit Tree Institute,Zhengzhou 450093,China;3.The Foodstuff Production Base of Military District in Inner Mongolia,Huhhot 010010,China)  
The Peach some varieties were used to adopt uniform design,the SRAP-PCR reaction system Taq DNA polymerase,the template DNA,dNTPs,Mg2+,primers five components to optimize the concentration.The results showed that 10 μ L Peach SRAP reaction system of the best components,including 2.5 μL 10×PCR Buffer+Mg2+,1 U Taq DNA polymerase,40 ng template DNA,0.2 mmol/L dNTPs,0.2 μmol/L primer.Established by the use of the system to other parts of peach germplasm to amplify the results of clear and reliable,good polymorphism.And using random selection of the SRAP primer and SSR primer respectively distinction between identification of eight kinds of peach germplasm and found that SRAP can be used to separate peach germplasm,and the SSR was used to separate,that the peach variety identification on the SRAP in more than SSR Convenient,and relatively simple operation,the applicability.
【CateGory Index】: S662.1
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Chinese Journal Full-text Database 6 Hits
1 GUO Ling-fei1,2,ZOU Ming-hong1,DU Li-qing1,ZENG Hui1,LU Chao-zhong1(1South Subtropical Crops Research Institute,CATAS,Zhanjiang,Guangdong 524091 China;2College of Horticulture,South China Tropical Agricultural University,Danzhou,Hainan 571737 China);Optimization of the sequence-related amplified polymorphism(SRAP) reaction system for macadamia by uniform design[J];Journal of Fruit Science;2008-02
2 SU Zhi-fang(Farming-grazing Science Department,Hetao University,Linhe 015000,China);SSR Marker and its Application in Wheat Genetic Diversity[J];Inner Mongolia Agricultural Science and Technology;2008-02
3 LI Jian-jun~1 LIU Zhi-jian~2 XIAO Ceng-lin~(1**) HE Li-wei~1 (1 Rice Research Institution,Hunan Agriculture University,Changsha,410128;2 Agricultural Bereau,Wangcheng County,Changsha,410200);Research progress on SRAP Technique in Genetics[J];Progress in Modern Biomedicine;2007-05
4 LI Xiao-hui1,TIAN Chao-yang2,WANG Cong-yan2(1.Institute of Horticulture,Henan Academy of Agriculture sciences, Zhengzhou 450002,China;2.College of Life Sciences, Henan Agricultural University ,Zhengzhou 450002,China);Genetic Polymorphism Analysis by Sequence-related Amplified Polymorphism Markers in Citrullus lanatus[J];Biotechnology;2007-03
5 Zhen Zhen1, Cao Qingqin2, Shen Yuanyue1, Qin Ling1 (1Department of Plant Science and Technology, Beijing Agriculture University, Beijing 102206; 2 Department of Biotechnology, Beijing Agricultural University, Beijing 102206 );SSR Technique and Application in Fruit Tree Research[J];Chinese Agricultural Science Bulletin;2007-06
6 PAN Qing-hua, HU Tie-zhu, WANG Ling, CAI Jiang-qiao (Laboratory of Plant Resistance and Genetics, College of Natural Resource Environment, South China Agricultural University, Guangzhou 510642);Molecular Genetic Studies on the Rice Blast Fungus Population DNA fingerprinting and pathotype analyses of the isolates selected from the region-specific lineages of Guangdong Province, China[J];Scientia Agricultura Sinica;2004-01
Chinese Journal Full-text Database 10 Hits
1 ZHAO Yang et al (Department of Horticulture,Agricultural College, Shihezi University, Shihezi,Xinjiang 832003);Study on Embryogenic Callus Induction from Guar[J];Journal of Anhui Agricultural Sciences;2005-02
2 ZHAO Xin-liang et al (Henan Institute of Science and Technology,Xinxiang,Henan 453003);Optimization of SRAP System in Maize[J];Journal of Anhui Agricultural Sciences;2006-15
3 ZHAO Xin-liang et al (Northwest A&F University,Yangling,Shaanxi 712100);Study on Classification of Maize Inbred Lines in Henan with SRAP Marker Technology[J];Journal of Anhui Agricultural Sciences;2007-07
4 DING Jin-ping et al (College of Agronomy,Guangxi University,Nanning,Guangxi 530003);Establishment and Optimization of SRAP Reaction Conditions for Arachis hypogaea L.[J];Journal of Anhui Agricultural Sciences;2008-10
5 YI Chuang-hui et al(Key Laboratory of Resource Insect Cultivation and Utilization,State Forestry Administration,Research Institute of Resources Insects,Chinese Academy of Forestry,Kunming,Yunnan 650224);The Main Influence Factors on the Development of Diapause Pupae of Papilio memnon[J];Journal of Anhui Agricultural Sciences;2009-02
6 LI Xiao-Jun et al(Life Science College,Hebei Normal University,Shijiazhuang,Hebei 050016);Studies on the Culture Condition and Fermentation Crafts of A Strain of Bacillus subtilis[J];Journal of Anhui Agricultural Sciences;2009-04
7 ZHOU Li et al (College of Horticulture and Forestry Science,Huazhong Agricultural University,Wuhan,Hubei 430070);Establishment and Optimization of ISSR-PCR Reaction System for the Hybrid of Cymbidium cyperifolim var.szechuanicum and C.floribundum[J];Journal of Anhui Agricultural Sciences;2009-17
8 DENG Bo-tao ZHAO Fei WANG Xue-ying (College of Biological Sciences and Technology,Shenyang Agricultural University,Shenyang Liaoning 110161);Optimization of SRAP in Carrot[J];;2009-13
9 FENG Yong,HE Jian-xin,LIU Liang,YANG Li-xing(College of Water Conservancy and Civil Engineering,Xinjiang Agricultural University,rümqi Xinjiang 830052,China);Experimental Study of the Shear Strength Characteristics of Fine-Grained Soil under Freezing and Thawing Cycles[J];Journal of Glaciology and Geocryology;2008-06
10 DING Wei-Dong,CAO Li-Ping ,CAO Zhe-Ming Key Open Laboratory for Genetic Breeding of Aquatic Animals and Aquaculture Biology,Ministry of Agriculture,Freshwater Fisheries Research Center of the Chinese Academy of Fishery Sciences,Wuxi 214081,Jiangsu Province,China;The SRAP and SCAR molecular markers for detecting germ degeneration in Ctenopharyngodon idellus[J];Acta Zoologica Sinica;2008-03
China Proceedings of conference Full-text Database 10 Hits
1 REN Yu YANG Guang-sui YIN Jun-mei (Tropical Crops Genetic Resources Institute,Chinese Academy of Tropical Agriculture Sciences,Danzhou 571737);Analysis of Genetic Diversity in Dendrobium Germplasm by SRAP Makers[A];[C];2007
2 ;SRAP Molecular Marker Related to Green Peel Gene in Cucumber[A];[C];2008
3 ;The Principle and Research Advancement of Sequence-Related Amplified Polymorphism[A];[C];2008
4 Zhang yongxing~1 Wang guilin~2(Civil engineering faculty of Chongqing university,Chongqing,400045);APPLICATION AND METHODS FOR STABILITY ANALYSIS OF HIGH-RISE BUILDING ROCK CAVERN FOUNDATION[A];[C];2007
5 FAN Shu-li, YU Shu-xun, SONG Mei-zhen(Cotton Research Institute, Chinese Academy of Agricultural Sciences; Key Laboratory of Cotton Genetic Improvement, Ministry of Agriculture, Anyang, He′nan 455004,China);Construction of Molecular Marker Linkage Map in Short-season Cotton and Earliness QTLs Mapping[A];[C];2005
6 ;利用微卫星标记分析天然棕色棉的遗传多样性[A];[C];2008
7 Yang Jifeng~1 Shi Jianwei~2 Qian Yiyu~1 Li Jin~2 (1.Harbin Institute of Technology,Harbin,150001,China 2.Sun East Electronic Technology Company Lt.d,Shenzhen,518103,China);The Influence of Solder Paste Printing Parameters in 0201 Assembly[A];[C];2007
8 ZHANG Hong-bo, GU Lei, XU You-zhong (Automobile Engineering Research Institute, CHERY Automobile Co., Ltd., WuHu, 241009);Evidence Theory Based Design Optimization For Vehicle Crashworthiness[A];[C];2007
9 Pian Rui-qi (Laboratory for Biotechnology in Tropical and Subtropical Forest Science; College of forestry; South China Agricultural University Guangzhou 510642; Biological Institute, Beijing Forestry University Beijing 100083) Ni Hui-qun Chen Xiao-yang (Biological Institute, Beijing Forestry University Beijing 100083);Optimization of SRAP System and Genetic Relationship Analysis of Lespedeza[A];[C];2009
10 CHEN Gang FENG Jian WANG Qian-chun PAN Wen-li FAN Jun-gang (Liaoning Research Academy of Forestry Sciences Shenyang 110032);SRAP Marker and Its Prospect of the Application in Forest Tree Genetic Breeding[A];[C];2009
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 XU Pei-wen 1,QU Shi-song 1,HUANG Bao-yong 1,SHI Yan-jing 2 LIU Heng-ying 1,ZHANG Jie 1,SUN Jin-bin,SONG Hong-ping 1 (1 Institute of Vegetable Research, Shandong Academy of Agricultural Sciences,Jinan 250100,China; 2 Department of Horticulture,;A preliminary study on genetic identification and purity assessments of garlic by RAPD[J];Shandong Agricultural Sciences;2002-01
2 LIU Yue-guang~(1*),TENG-Yong yong~(3*),PAN Chen~1,HAN Yan-chuang~1,ZHOU Ming-quan~2,HU Zhong-li~(1,2**)(1 Key Lab of the Ministry of Education for Plant Developmental Biology,Wuhan University,Wuhan 430072;2 Lotus center,Wuhan University,Wuhan 430072;3 College of Life Sciellce,Xinyang Nomal University,Xinyang 46400,China);Cluster Analysis of Nelumbo Based on SRAP Markers[J];Amino Acids & Biotic Resources;2006-01
3 WU Zhi-pu,YANG Wen-xiang,LIU Da-qun,ZHANG Ting (Department of Plant Pathology, Agricultural University of Hebei, Biological Control Center of Plant Pathogens and Plant Pests of Hebei Province, Baoding 071001, China);Establishment of SRAP technique system in wheat genome[J];Journal of Agricultural University of Hebei;2005-03
4 LIANG Hai-yong~1,LIU Cai-xia~2,LIU Xing-ju~1,YANG Min-sheng~1(1.College of Forestry, Agricultural University of Hebei, Baoding 071001, China;2. Chinese Academy of Forestry, Beijing 100091, China);Simple sequence repeat (SSR) analysis and identify of different cultivars in Populus L.[J];Journal of Agricultural University of Hebei;2005-04
5 PENG Sa~(1,2),GUO Mei-li~(1*),CHEN Yue-hua~(2,3),GUO Qing-hua~(1)(1.Department of Pharmacognosy,School of Pharmacy,Second Military Medical University,Shanghai 200433,China;2.College of Agronomy,Xinjiang Agricultural University,Urumchi 830000;3.Institute of Economic Crop,Xinjiang Academy of Agricultural Sciences,Urumchi 830000);Establishment and optimization of sequence-related amplified polymorphism amplification system for Carthamus tinctorius L.[J];Academic Journal of Second Military Medical University;2006-05
6 LI Shan, ZHAO Guifang(Biodiversity research center of Mts. Qinling, Northwest University, Xi'an,710069,China);AFLP molecular marker and its application[J];Acta Botanica Boreali-occidentalia Sinica;2003-05
7 LI Li,PENG Jian-ying~*,BAI Rui-xia,HAN Bin(College of Horticulture,Agricultural University of Hebei,Baoding,Hebei 071001,China);SRAP and TRAP Markers and their Application to Research about Horticultural Plants[J];Acta Botanica Boreali-Occidentalia Sinica;2006-08
8 Ren Yu1,2 Wang Deyuan2* Zhang Yindong1 Li Ying2 Wang Hengming21 State Key Laboratory of Tropical Crop Biotechnology, CATAS, Haikou, 5711012 Vegetable Institute, Guangdong Academy of Agricultural Sciences, Guangdong Key Biotech Lab for Fruits and Vegetables, Guangzhou, 510640*Corresponding author, wdy168@tom.com;Optimization of SRAP-PCR in Hot Pepper (Capsicum annuum L)[J];Molecular Plant Breeding;2004-05
9 Sun Hong1 Cheng Jing1 Zhan Kehui1* Zhang Xiangwu2 Yang Yanhui11 College of Agronomy, Henan Agricultural University, Zhengzhou, 450002; 2 Xihua Research Institute of Agricultural Sciences in Henan Province, Xihua County, 466600* Corresponding author, kh486@163.com;ISSR Marker and its Application in Crop Genetics and Breeding[J];Molecular Plant Breeding;2005-01
10 Wu Jie1 Tan Wenfang2 He Junrong1 Pu Zhigang1 Wang Dayi2 Zhang Zhengsheng3 Zhang Fufeng4 Yan Wenzhao1* 1 Institute of Biotechnology and Nuclear Technology, Sichuan Academy of Agricult ural Sciences, Chengdu, 610066; 2 Crop Institute, Sichuan Academy of Agricultur e Sciences, Chengdu, 610066; 3 Biotechnology Research Center, Southwest Agricult ural University, Chongqing, 400716; 4 College of life Science, Sichuan Universit y, Chengdu, 610064;Construct on of SRAP Linkage Map and QTL Mapping for Starch Content in Sweet Potato[J];Molecular Plant Breeding;2005-06
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